Expression of feruloyl esterase from Aspergillus awamori in Escherichia coli: Characterization and crystal studies of the recombinant enzyme

Fazary, Ahmed E.; Hamad, Hamed A.; Lee, John C.; Koskei, Takuya; Lee, Cheng‐Kang; Ju, Yi‐Hsu

doi:10.1016/j.ijbiomac.2010.01.020

Cited by 13 publications

(4 citation statements)

References 23 publications

(37 reference statements)

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“…Lignocellulosic fractions of these by‐products can act as inductors or inhibitors of the production of extracellular enzymes in SSF, depending on their concentration 7 . This complex matrix is recalcitrant to degradation due to the covalent bonds between hemicellulose and lignin established by hydroxycinnamic acids such as ferulic acid 8 . Phenolic compounds can be found within the matrix of plant cells in different forms: free phenolics, soluble phenolics (conjugated) or insoluble phenolics.…”

Section: Introductionmentioning

confidence: 99%

“…7 This complex matrix is recalcitrant to degradation due to the covalent bonds between hemicellulose and lignin established by hydroxycinnamic acids such as ferulic acid. 8 Phenolic compounds can be found within the matrix of plant cells in different forms: free phenolics, soluble phenolics (conjugated) or insoluble phenolics. Soluble phenolics are normally esterified to soluble compounds with low molecular weight such as carbohydrates, proteins or lipids.…”

Section: Introductionmentioning

confidence: 99%

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Degradation of lignocellulosic matrix of oilseed cakes by solid‐state fermentation: fungi screening for enzymes production and antioxidants release

et al. 2021

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BACKGROUND: Vegetable oils are yearly produced in large amounts generating solid by-products, the oilseed cake (OC). OCs are lignocellulosic materials that have been used for animal feed with some limitations due to high fibre content from the plant cell walls. Biotechnological processes can help to overcome these limitations and contribute to up-grading such by-products, enhancing their nutritional value as feed ingredients.RESULTS: All fungal species were able to decrease neutral detergent fibre and acid detergent fibre in all by-products. Additionally, relevant enzymes were produced by the three fungi studied resulting in an improved antioxidant capacity of all fermented OCs. Aspergillus niger led to the highest activity of cellulase (109 U g −1 ), xylanase (692 U g −1 ) and protease (157 U g −1 ) per dry OC matter and to the recovery of an extract rich in antioxidants, with the highest scavenging potential of free radicals and superoxide anion, iron chelation ability and reducing power. Rhyzopus oryzae produced the highest activity of ⊎-glucosidase (503 U g −1 ) and led to the highest liberation of total phenolic content (TPC). Principal components analysis showed that extracts with high antioxidant potential were obtained in solid-state fermentation (SSF) with high enzymatic activity. A positive correlation was established between the action of ⊎-glucosidase and TPC.CONCLUSION: Within the same bioprocess it was possible to improve the nutritional value of OCs and to obtain relevant bioactive compounds such as lignocellulosic enzymes and phenolic compounds with antioxidant potential, resulting in a significant improvement of already valuable by-products with commercial interest for animal feed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Degradation of lignocellulosic matrix of oilseed cakes by solid‐state fermentation: fungi screening for enzymes production and antioxidants release

et al. 2021

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“…Furthermore, grape pomace could induce the production of laccase for Trametes versicolor [9]. Some species of microorganisms belonging to the Aspergillus genus can produce tannase [10], naringinase [11], β-glucosidase [12], feruloyl esterase [13] and flavonol 2,4-dioxygenase [14], which make it possible to transform bioactive compounds. Hung et al [15] have found that the antimutagenic activity of black bean can be increased after the treatment with Aspergillus.…”

Section: Introductionmentioning

confidence: 99%

Enhanced DPPH radical scavenging activity and DNA protection effect of litchi pericarp extract by Aspergillus awamoribioconversion

Lin

Yang

Chen

et al. 2012

Chemistry Central Journal

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BackgroundLitchi (Litchi chinensis Sonn.) pericarp is a major byproduct which contains a significant amount of polyphenol. This study was designed to biotransformation litchi pericarp extract (LPE) by Aspergillus awamori to produce more bioactive compounds with stronger antioxidant activities.ResultsThe study exhibited that the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities significantly (p < 0.05) increased from 15.53% to 18.23% in the water-extracted fraction and from 25.41% to 36.82% in the ethyl acetate-extracted fraction. Application of DNA cleavage assay further demonstrated the enhanced protection effect of the fermented phenolics on DNA damage. It is also noted that the water-extracted fraction of the fermented LPE possessed a much stronger capacity than the ethyl acetate-extracted fraction to prevent from damage of supercoiled DNA. Interestingly, it was found that some new compounds such as catechin and quercetin appeared after of A. awamori fermentation of LPE, which could account for the enhanced antioxidant activity.ConclusionThe DPPH radical scavenging activity and DNA protection effect of LPE were increased by Aspergillus awamori bioconversion while some compounds responsible for the enhanced antioxidant activity were identified. This study provided an effective way of utilizing fruit pericarp as a readily accessible source of the natural antioxidants in food industry and, thus, extended the application area such as fruit by-products.

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“…The purity of the expressed proteins was 95%, 217 which was higher than that of feruloyl esterase from A. oryzae (86%) 218 [12]. [12] and A. niger CBS 227 120.49 (40.0 kDa) [15], but larger than those of feruloyl esterases from 228 A. usamii (36.0 kDa) [13], A. oryzae (37.0 kDa) [14] and Aspergillus 229 awamori (35.0 kDa) [16]. The apparent molecular weight of expressed 230 protein was much larger than the calculated molecular mass of 231 28.6 kDa, which was probably due to glycosylation.…”

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confidence: 99%

Construction and expression of two-copy engineered yeast of feruloyl esterase

Zhou

Huang

Zhou

et al. 2015

Electronic Journal of Biotechnology

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Expression of feruloyl esterase from Aspergillus awamori in Escherichia coli: Characterization and crystal studies of the recombinant enzyme

Cited by 13 publications

References 23 publications

Degradation of lignocellulosic matrix of oilseed cakes by solid‐state fermentation: fungi screening for enzymes production and antioxidants release

Degradation of lignocellulosic matrix of oilseed cakes by solid‐state fermentation: fungi screening for enzymes production and antioxidants release

Enhanced DPPH radical scavenging activity and DNA protection effect of litchi pericarp extract by Aspergillus awamoribioconversion

Construction and expression of two-copy engineered yeast of feruloyl esterase

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