Expression of extracellular matrix metalloproteinase inducer and enhancement of the production of matrix metalloproteinases in rheumatoid arthritis

Abstract

Objective.To investigate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) at sites of joint destruction in rheumatoid arthritis (RA) and to correlate it with the production of matrix metalloproteinases (MMPs).Methods. Reverse transcription-polymerase chain reaction was performed to study the existence of EMMPRIN in synovial tissue derived from RA and osteoarthritis (OA) patients. In situ hybridization with a human complementary DNA specific for EMMPRIN and immunohistochemistry were performed to characterize the EMMPRIN-expressing cells at sites of joint destruction, including bone. Northern blot analysis was performed to detect the level of expression of EMMPRIN messenger RNA (mRNA) in synovial tissue. The production of MMP-1 and MMP-3 by synovial tissue from RA patients was examined by enzyme-linked immunosorbent assay.Results. Expression of EMMPRIN mRNA was detected in synovium from 9 of 11 patients with RA and 1 of 5 patients with OA. The presence of mRNA encoding EMMPRIN was recognized in the invasive synovium at sites of joint destruction in RA but not OA.

Extracellular Cyclophilins Contribute to the Regulation of Inflammatory Responses

“…One potential explanation is that CD147 expression is down-regulated on leukocytes after their entry into tissues, supporting the idea that CD147 is most important for the recruitment phase of leukocytes during an inflammatory response. However, other studies have reported elevated levels of CD147 on leukocytes present within inflammatory sites, including fibroblastlike cells and granulocytes in the synovial joints of rheumatoid arthritis patients (16,17). Whether these differences in expression contribute in any way to the recruitment and/or entry of leukocytes into tissues has not been established; however, using in vitro chemotaxis assays, we have observed no difference in the capacity of neutrophils from the circulation vs different tissues to migrate in response to CyPA (K. Arora, unpublished observations).…”

Membrane-Type I Matrix Metalloproteinase-Dependent Regulation of Rheumatoid Arthritis Synoviocyte Function

“…The precise role, however, that these enzymes play in RA synoviocyte-mediated tissue destruction remains the subject of speculation. Despite the facts that RA synoviocytes are currently believed to mediate the bulk of the tissue-destructive effects associated with this disease state (1)(2)(3)(4) and that the isolated cells can be shown to express multiple proteolytic enzymes in vitro (19)(20)(21)(22)(23)(24)(25)(26)(27), only a handful of studies have attempted to examine the type I/II collagenolytic properties of isolated synovial fibroblasts or tissues (28)(29)(30). In the few cases where the collagenolytic activity of RA synoviocytes has been documented (28)(29)(30), the proteolytic systems responsible for collagen degradation have not been identified directly.…”

Expression of extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in pancreatic neoplasm and pancreatic stellate cells

“…21,22 In addition, EMMPRIN also plays a role in tumor invasion, 17,[23][24][25] angiogenesis, 24,26 apoptosis 27 and chemoresistance, 28 as well as in nonmalignant diseases such as rheumatoid arthritis, [29][30][31] chronic liver disease, 32,33 heart failure 34,35 and atherosclerosis. 35 In the present study, the expression of EMMPRIN was analyzed in the normal pancreas, chronic pancreatitis (CP), and in various pancreatic tumors, and its functional role was specifically assessed in pancreatic cancer.…”