Expression of exogenous protein in the egg white of transgenic chickens

Harvey, Alex J.; Speksnijder, G.; Baugh, L. Ryan; Morris, Julie; Ivarie, Robert

doi:10.1038/nbt0402-396

Cited by 157 publications

(82 citation statements)

References 16 publications

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“…defective avian leukosis virus was used to generate hens expressing the reporter ␤-lactamase from a ubiquitous promoter. While ␤-lactamase was detected in egg white of transgenic hens, the quantity of protein, efficiency of transgenesis, and germ-line transmission rate were all low (17,18). Arian leukosis virus was also used to generate chickens expressing human IFN-␣-2b at higher levels than had been reported for ␤-lactamase (3), but the efficiency with which transgenic birds were generated remained very low (a single G 1 cockerel from 1,597 chicks hatched), presumably a consequence of the low-titer vector used.…”

Section: Discussionmentioning

confidence: 99%

Oviduct-specific expression of two therapeutic proteins in transgenic hens

Lillico

Sherman

McGrew

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

185

168

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Recent advances in avian transgenesis have led to the possibility of utilizing the laying hen as a production platform for the large-scale synthesis of pharmaceutical proteins. Ovalbumin constitutes more than half of the protein in the white of a laid egg, and expression of the ovalbumin gene is restricted to the tubular gland cells of the oviduct. Here we describe the use of lentiviral vectors to deliver transgene constructs comprising regulatory sequences from the ovalbumin gene designed to direct synthesis of associated therapeutic proteins to the oviduct. We report the generation of transgenic hens that synthesize functional recombinant pharmaceutical protein in a tightly regulated tissue-specific manner, without any evidence of transgene silencing after germ-line transmission.chicken ͉ genetic modification ͉ lentivirus ͉ pharmaceutical

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Section: Discussionmentioning

confidence: 99%

Oviduct-specific expression of two therapeutic proteins in transgenic hens

Lillico

Sherman

McGrew

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

185

168

View full text Add to dashboard Cite

show abstract

“…Transgenic birds generated by using oncoretroviral vectors have been shown to express low levels of transgene in the egg whites of laid eggs (32). The chicken ovalbumin promoter has been suggested as a regulatory sequence for directing protein expression in egg whites (33).…”

Section: Discussionmentioning

confidence: 99%

Generation of tissue-specific transgenic birds with lentiviral vectors

Scott

Lois

2005

Proc. Natl. Acad. Sci. U.S.A.

133

109

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Birds are of great interest for a variety of research purposes, and effective methods for manipulating the avian genome would greatly accelerate progress in fields that rely on birds as model systems for biological research, such as developmental biology and behavioral neurobiology. Here, we describe a simple and effective method for producing transgenic birds. We used lentiviral vectors to produce transgenic quails that express GFP driven by the human synapsin gene I promoter. Expression of GFP was specific to neurons and consistent across multiple generations. Expression was sufficient to allow visualization of individual axons and dendrites of neurons in vivo by intrinsic GFP fluorescence. Tissuespecific transgene expression at high levels provides a powerful tool for biological research and opens new avenues for genetic manipulation in birds.avian ͉ genetics ͉ lentivirus ͉ synapsin ͉ neuron

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“…Since Salter et al (1987) first demonstrated the production of transgenic chickens by injecting avian leukosis viruses into blastoderm, many types of virus such as retrovirus and lentivirus were attempted to generate the transgenic birds. Using a replication-deficient retroviral vector based on the avian leukosis virus (ALV), Harvey et al (2002) reported that the biofunational β-lactamase was secreted and deposited into egg white of four generations of transgenic chickens which were transferred the transgene controlled by the ubiquitous cytomegalovirus (CMV) promoter.…”

Section: Introductionmentioning

confidence: 99%

Non-Viral Transgenesis via Direct In Ovo Lipofection in Quail

Park

Han²

2015

Korean Journal of Poultry Science

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Transgenic animals have been widely used for developmental biology studies, as disease models, and even in industry such as transgenic bioreactor animals. For transgenic birds, quail has the great advantages of small body size, short generation time, and frequent egg production. To date, retroviral or lentiviral transduction has been used to generate transgenic quail for various purposes. However, the efficiency of transgenic offspring production with these methods is relatively low and viral vector usage has safety issues. Unfortunately, non-viral transgenesis has not been established in quail due to a deficiency of stem cell and germ cell culture systems. In this study, we established a direct in ovo lipofection method that could be used to create transgenic quail without germline-competent cells or viruses. To optimize the injection stage during embryo development, the liposome complex (containing piggyBacCMV-GFP and transposase plasmids) was introduced into an embryonic blood vessel at 50 hr, 55 hr or 60 hr. GFP expression was detected in various tissues (heart, kidney, liver and stomach) on day 12 of incubation under a fluorescence microscope. Additionally, GFP-positive cells were detected in the recipient embryonic gonads. In conclusion, the direct in ovo lipofection method with the piggyBac transposon could be an efficient and useful tool for generating transgenic quail.

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Expression of exogenous protein in the egg white of transgenic chickens

Cited by 157 publications

References 16 publications

Oviduct-specific expression of two therapeutic proteins in transgenic hens

Oviduct-specific expression of two therapeutic proteins in transgenic hens

Generation of tissue-specific transgenic birds with lentiviral vectors

Non-Viral Transgenesis via Direct In Ovo Lipofection in Quail

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