Expression of ETS-1 Related to Angiogenesis in Uterine Endometrium during the Menstrual Cycle

Abstract

ETS-1 has been identified as a transcription factor for angiogenesis, which is essential for the development and growth of the uterine endometrium. This characteristic prompted us to study whether ETS-1 functions as an angiogenic mediator in uterine endometrium. Immunohistochemical staining revealed that the localization of ETS-1 was similar to that of vascular endothelial cells. There was a significant correlation between microvessel counts and both ETS-1 histoscores and ets-1 mRNA levels in uterine endometrium. The ETS-1 histoscores and ets-1 mRNA levels increased in the proliferative phase, reached a peak during peri-ovulation and decreased in the secretory phase. Furthermore, the ETS-1 histoscores and ets-1 mRNA levels correlated with vascular endothelial growth factor (VEGF) levels in the proliferative phase. This indicates that ETS-1 might be an angiogenic mediator in uterine endometrium linked to VEGF in the proliferative phase.

Oxidized Phospholipids Increase Interleukin 8 (IL-8) Synthesis by Activation of the c-src/Signal Transducers and Activators of Transcription (STAT)3 Pathway

“…With the exception of STAT proteins, all of these transcription factors have been associated with up-regulation of IL-8 in various conditions and cell types (8,(32)(33)(34). We have provided evidence that a known downstream effector of c-src kinase, STAT3, is activated by Ox-PAPC treatment in human endothelial cells.…”

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“…In addition, inhibition of Ets1 activity was shown to suppress hepatocyte growth factor (HGF) and FGF-2 induced angiogenesis in animals [114,115]. During the menstrual cycle, EC-dependent Ets1 expression increases in the proliferative phase and drops in the secretory phase, following changes in the VEGF level [116]. During pregnancy, Ets1 is found in endothelial cells of the villous trophoblast, but not in ECs of maternal endothelial cells [117].…”

Ets-1 Is Required for the Activation of VEGFR3 during Latent Kaposi's Sarcoma-Associated Herpesvirus Infection of Endothelial Cells

“…Ets-1 is found in a variety of cell and tissue types, including lymphocytes, fibroblasts, mesenchymal cells, and endothelial cells of the developing vasculature (30)(31)(32)(33)(34). Ets-1 induces angiogenic and invasive phenotypes in endothelial cells, including wound healing, cell proliferation, and cell survival during embryonic angiogenesis (35)(36)(37)(38).…”