Expression of ethylene response genes during persimmon fruit astringency removal

Abstract

Thirteen ethylene signaling related genes were isolated and studied during ripening of non-astringent 'Yangfeng' and astringent 'Mopan' persimmon fruit. Some of these genes were characterized as ethylene responsive. Treatments, including ethylene and CO(2), had different effects on persimmon ripening, but overlapping roles in astringency removal, such as increasing the reduction in levels of soluble tannins. DkERS1, DkETR2, and DkERF8, may participate in persimmon fruit ripening and softening. The expression patterns of DkETR2, DkERF4, and DkERF5 had significant correlations with decreases in soluble tannins in 'Mopan' persimmon fruit, suggesting that these genes might be key components in persimmon fruit astringency removal and be the linkage between different treatments, while DkERF1 and DkERF6 may be specifically involved in CO(2) induced astringency removal. The possible roles of ethylene signaling genes in persimmon fruit astringency removal are discussed.

A Plant Phytosulfokine Peptide Initiates Auxin-Dependent Immunity through Cytosolic Ca2+ Signaling in Tomato

“…Ethylene production was measured as described previously (Yin et al, 2012). Briefly, tomato seedlings were sealed in 500-mL rubber-topped flasks for 1 h at 20°C, and then 1 mL of head-space gas was removed and injected into a gas chromatograph (Agilent 6890N; Agilent Technologies) fitted with a Proapack-Q column.…”

Perlakuan Pascapanen Buah Kesemek Reundeu (Diosphyros kaki L.) Menggunakan Gas Karbon Dioksida

“…Tanin akan larut dalam air khususnya air panas dan pelarut organik seperti methanol, etanol dan aseton (Wardani dan Leviana, 2010;Budi et al, 2012). Kandungan tanin yang tinggi menyebabkan buah akan tetap sepat meskipun telah matang di pohon (Yonemori dan Suzuki, 2008;Yin et al, 2012) sehingga buah klimakterik ini sulit untuk dikonsumsi.…”

“…Perlakuan gas CO 2 pada beberapa penelitian dianggap lebih efektif untuk menurunkan kadar tanin buah kesemek dibandingkan dengan perlakuan lainnya (Ahmed dan Sobieh, 2007;Yin et al, 2012;Ghidelli et al, 2013) sehingga berpotensi untuk diterapkan pada kultivar kesemek yang ada di Indonesia. Efektifitas penggunaan gas CO 2 konsentrasi tinggi yang bersumber dari dry ice didasarkan pada peningkatan metabolisme buah yang memicu insolubilitas molekul tanin terkondensasi terhadap asetaldehid yang dihasilkan selama respirasi (Novillo et al, 2014).…”

Harvest and Postharvest Quality of Persimmon Fruit:physicochemical and Nutritional Aspects.

“…The biological effects of ethylene are mediated through a signal transduction pathway in which ethylene receptors are involved (Yin et al, 2012). Fig.5 shows the relative expression of ethylene receptors genes DkETR1, DkETR2 and DkERS1 in fruit sampled after 1, 4 and 7 days of shelf-life at 20 ºC.…”

“…The implication of the oxidative system in CI sensitivity has been studied in depth in other fruits, in which chilling manifestation and its reduction by different treatments have been related to changes in ROS and enzymes of the oxidative system. This is the case of loquats (Cao et al, 2009(Cao et al, , 2011Wang et al, 2005;Xu et al, 2012), apple (Rupasinghe et al, 2000) or mandarins (Sala, 1998;Sala andLafuente, 1999, 2000).…”