Expression of estrogen receptors (α, β) and androgen receptor in serotonin neurons of the rat and mouse dorsal raphe nuclei; sex and species differences

Sheng, Zijing; Kawano, June; Yanai, Akie; Fujinaga, Ryutaro; Tanaka, Mayumi; Watanabe, Yoshifumi; Shinoda, Koh

doi:10.1016/j.neures.2004.02.011

Cited by 102 publications

(71 citation statements)

References 59 publications

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“…An electrophysiological study revealed that firing activity of the 5-HT neurons in the DRN, but not MRN, was higher in the females than males (48). Additionally, several studies revealed that gonadal steroid hormones such as androgen and estrogen can regulate neural activity in the DRN in a genderspecific manner (66,67). Thus, the region-specific 5-HTergic mechanisms in the neural circuit including the BLA and hippocampus may underlie gender differences in emotional regulation (68,69).…”

Section: Discussionmentioning

confidence: 99%

Metaplastic Regulation of the Median Raphe Nucleus via Serotonin 5-HT1A Receptor on Hippocampal Synaptic Plasticity Is Associated With Gender-Specific Emotional Expression in Rats

et al. 2014

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Abstract. Gender differences in psychiatric disorders are considered to be associated with the serotonergic (5-HTergic) system; however the underlying mechanisms have not been clearly elucidated. In this study, possible involvement of the median raphe nucleus (MRN)-hippocampus 5-HTergic system in gender-specific emotional regulation was investigated, focusing on synaptic plasticity in rats. A behavioral study using a contextual fear conditioning (CFC) paradigm showed that the females exhibited low anxiety-like behavior. Extracellular 5-HT levels in the hippocampus were increased by CFC only in the males. Long-term potentiation (LTP) in the hippocampal CA1 field was suppressed after CFC in the males, which was mimicked by the synaptic response to MRN electrical stimulation. In the MRN, 5-HT immunoreactive cells significantly increased in the females compared with those in the males. Pretreatment with the 5-HT 1A receptor agonists tandospirone (10 mg/kg, i.p.) and 8-OH DPAT (3 mg/kg, i.p.) significantly suppressed LTP induction in the males. Synaptic responses to CFC and 5-HT 1A receptor interventions were not observed in the females. These results suggest that the metaplastic 5-HTergic mechanism via 5-HT 1A receptors in the MRN-hippocampus pathway is a key component for gender-specific emotional regulation and may be a cause of psychiatric disorders associated with vulnerability or resistance to emotional stress.

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Section: Discussionmentioning

confidence: 99%

Metaplastic Regulation of the Median Raphe Nucleus via Serotonin 5-HT1A Receptor on Hippocampal Synaptic Plasticity Is Associated With Gender-Specific Emotional Expression in Rats

et al. 2014

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“…This pattern suggests not only estradiolmediated regulation of hippocampal input and output signaling in the dentate gyrus and subiculum, but also that this regulation is primarily via the ERβ (Östlund et al 2003). ERβ immunoreactivity has been observed in the dorsal raphe nucleus (Mitra et al 2003;Sheng et al 2004). Using in vivo extracellular unitary recordings of rat dorsal raphe nucleus 5-HT neurons, Robichaud and Debonnel (2005) showed that 17β-estradiol, and testosterone, increased the firing activity of 5-HT neurons in both males and females.…”

Section: Discussionmentioning

confidence: 99%

The antidepressant-like effect of the 3β-hydroxysteroid dehydrogenase inhibitor trilostane involves a regulation of β-type estrogen receptors

Espallergues

Temsamani²,

Laruelle³

et al. 2010

Psychopharmacology

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“…Double immunofluorescent staining was performed for co-localization studies. Sections were pre-incubated, bleached (18), and stained with a mixture of antiWfs1n (diluted 1:200) and mouse monoclonal antiinsulin (diluted 1:100; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-glucagon (diluted 1:200; Sigma-Aldrich, St Louis, MO, USA), or anti-somatostatin (diluted 1:25; Biomeda Corporation, Foster City, CA, USA) in 0.1 M sodium phosphate buffer containing 0.3% Triton X-100, 0.1% sodium azide, and 3% normal goat serum (PBT-NGS) for 24 h at 20 8C. Next, the sections were incubated with a mixture of two secondary antibodies in PBT-NGS for 24 h at 20 8C.…”

Section: Methodsmentioning

confidence: 99%

Endoplasmic reticulum stress induces Wfs1 gene expression in pancreatic β-cells via transcriptional activation

et al. 2005

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Objective: The WFS1 gene encodes an endoplasmic reticulum (ER) membrane-embedded protein.Homozygous WFS1 gene mutations cause Wolfram syndrome, characterized by insulin-deficient diabetes mellitus and optic atropy. Pancreatic b-cells are selectively lost from the patient's islets. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. Disturbances or overloading of these functions induces ER stress responses, including apoptosis. We speculated that WFS1 protein might be involved in these ER stress responses. Design and methods: Islet expression of the Wfs1 protein was analyzed immunohistochemically. Induction of Wfs1 upon ER stress was examined by Northern and Western blot analyses using three different models: human skin fibroblasts, mouse pancreatic b-cell-derived MIN6 cells, and Akita mouse-derived Ins2 96Y/Y insulinoma cells. The human WFS1 gene promoter-luciferase reporter analysis was also conducted. Result: Islet b-cells were the major site of Wfs1 expression. This expression was also found in d-cells, but not in a-cells. WFS1 expression was transcriptionally up-regulated by ER stress-inducing chemical insults. Treatment of fibroblasts and MIN6 cells with thapsigargin or tunicamycin increased WFS1 mRNA. WFS1 protein also increased in response to thapsigargin treatment in these cells. WFS1 gene expression was also increased in Ins2 96Y/Y insulinoma cells. In these cells, ER stress was intrinsically induced by mutant insulin expression. The WFS1 gene promoter-luciferase reporter system revealed that the human WFS1 promoter was activated by chemically induced ER stress in MIN6 cells, and that the promoter was more active in Ins2 96Y/Y cells than Ins2 wild/wild cells. Conclusion: Wfs1 expression, which is localized to b-and d-cells in pancreatic islets, increases in response to ER stress, suggesting a functional link between Wfs1 and ER stress.

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Expression of estrogen receptors (α, β) and androgen receptor in serotonin neurons of the rat and mouse dorsal raphe nuclei; sex and species differences

Cited by 102 publications

References 59 publications

Metaplastic Regulation of the Median Raphe Nucleus via Serotonin 5-HT1A Receptor on Hippocampal Synaptic Plasticity Is Associated With Gender-Specific Emotional Expression in Rats

Metaplastic Regulation of the Median Raphe Nucleus via Serotonin 5-HT1A Receptor on Hippocampal Synaptic Plasticity Is Associated With Gender-Specific Emotional Expression in Rats

The antidepressant-like effect of the 3β-hydroxysteroid dehydrogenase inhibitor trilostane involves a regulation of β-type estrogen receptors

Endoplasmic reticulum stress induces Wfs1 gene expression in pancreatic β-cells via transcriptional activation

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