Expression of Epidermal Growth-Factor Receptor Correlates With Metastatic Potential of 13762nf Rat Mammary Adenocarcinoma Cells

A Paracrine Loop between Tumor Cells and Macrophages Is Required for Tumor Cell Migration in Mammary Tumors

“…Overexpression of the EGF receptor has been shown to correlate with metastasis and poor prognosis in a number of tumor types, including small-cell lung cancer, breast cancer, gastric cancer, and prostate cancer (16 -19). Cell lines that overexpress EGF receptors also are more metastatic in vivo (20), and experimental expression of the EGF receptor in nonmetastatic cells increases their chemotactic responses to EGF in vitro and metastatic ability in vivo (21,22). Therefore, EGF receptor-mediated chemotaxis within the primary tumor may be important in enhancing invasion, intravasation, and metastasis in addition to the well-characterized effects of EGF receptor signaling on mitogenesis.…”

Chemoattractant-induced lamellipod extension

“…These cells grow easily in tissue culture and, when injected into the mammary fat pad of syngeneic Fisher 344 rats, form first a primary tumor and then lymph node and lung metastases. MTLn3 cells express roughly 50,000 EGF receptors per cell, while a nonmetastatic derivative termed MTC from the same original tumor does not express EGF receptors (Kaufmann et al, 1994). Expression of the EGF receptor in the MTC cells increases metastatic ability without affecting primary tumor growth rate (Kaufmann et al, 1996).…”

Isolation of Motile Tumor Cells from Live Breast Tumors

“…MTLn3 cells are chemotactic toward EGF in vitro, whereas MTC cells are not (45). MTLn3 cells express more EGF receptors than MTC cells (46), and expression of the EGF receptor in MTC cells increases chemotactic responses to EGF and metastatic ability (47,48). Expression of the EGF receptor and other EGF receptor homologues, such as ErbB2, have been correlated with poor prognosis.…”