Expression of Epidermal Growth Factor, Transforming Growth Factor‐α and Their Receptor Genes in Human Gastric Carcinomas; Implication for Autocrine Growth

Yoshida, Kazuhiro; Kyo, Eikai; Tsujino, Tetsuhiro; Sendo, Toshiaki; Niimoto, Minoru; Tahara, Eiichi

doi:10.1111/j.1349-7006.1990.tb02505.x

Cited by 91 publications

(30 citation statements)

References 31 publications

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“…al., 1990;Yasui et al, 1988;Tanimoto et al, 1991;Hattori et al, 1994;Shibamoto et al, 1992;Ito et al, 1992;Yoshida et al, 1989). Most scirrhous gastric cancer cell lines are known to be stimulated by EGF, b-FGF and KGF (Yoshida et al, 1990;Yasui et al, 1988;Tanimoto et al, 1991;Hattori et al, 1994). The growth of OCUM-2M cells was also stimulated by EGF, TGF-ax, VEGF, b-FGF and KGF, but not IGF-I, PDGF, HGF and TGF-fJ.…”

Section: Resultsmentioning

confidence: 99%

“…The fractions were collected at a flow rate of 1 ml min-'. (Yoshida et al, 1989(Yoshida et al, , 1990Yasui et al, 1988;Tanimoto et al, 1991;Hattori et al, 1994;Shibamoto et al, 1992;Ito et al, 1992) on the growth of OCUM-2M cells. An aliquot of 1 ml of OCUM-2M cell suspension (1.0 x 104 cells ml-') was inoculated into each well of 24-well plates (Falcon) with various concentrations of the defined growth factors, and incubated.…”

Section: Gel Filtration Chromatographymentioning

confidence: 99%

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Differential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts

Yashiro

Chung²,

Kubo³

et al. 1996

Br J Cancer

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Gastric fibroblasts specifically stimulated the growth of scirrhous gastric cancer cells, but not that of welldifferentiated adenocarcinoma cells. The growth factor(s) produced from gastric fibroblasts were then partially purified and characterised. The growth-promoting factor(s) had apparent molecular weights of 10 000 dalton and was sensitive both to heat and proteinase treatment. No inhibition for the factor(s) was achieved with defined anti-growth factor antibodies. In this study, differential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts were shown. Rapid proliferation of scirrhous gastric carcinoma should be partly controlled by orthotopic fibroblasts. The growth factor(s) from gastric fibroblasts, which was distinct from various defined growth factors such as epidermal growth factor (EGF), basic fibroblast growth factor (b-FGF), transforming growth factor-a (TGF-a), keratinocyte growth factor (KGF), vascular endothelial growth factor (VEGF), insulin-like growth factor I (IGF-I), hepatocyte growth factor (HGF), platelet-derived growth factor (PDGF) and transforming growth factor ,B1 (TGF-,B1) may play an important role in the progression of scirrhous gastric cancer cells.

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Section: Resultsmentioning

confidence: 99%

Section: Gel Filtration Chromatographymentioning

confidence: 99%

Differential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts

Yashiro

Chung²,

Kubo³

et al. 1996

Br J Cancer

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“…In particular, the epidermal growth factor receptor (EGF-R) pathway appears to play a crucial role in gastric cancer progression. A large percentage of gastric cancer cell lines express EGF-R (Yokozaki, 2000), and gastric cancer cells grow in response to EGF/transforming growth factor-a (TGF-a) activation of EGF-R in an autocrine loop (Yoshida et al, 1990;Piontek et al, 1993). Expression of EGF and its receptor has been found to correlate with prognosis in patients with gastric cancer (Yasui et al, 1988;Jonjic et al, 1997).…”

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confidence: 99%

Induction of neuropilin-1 and vascular endothelial growth factor by epidermal growth factor in human gastric cancer cells

et al. 2003

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The epidermal growth factor receptor (EGF-R) pathway plays a pivotal role in the progression of human gastric cancer. The angiogenic factor vascular endothelial growth factor (VEGF) has been shown to be induced by EGF in various cancer cell lines. Neuropilin-1 (NRP-1) acts as a coreceptor for VEGF-165 and increases its affinity for VEGF receptor 2 (VEGFR-2) in endothelial cells. Furthermore, NRP-1 has been found to be expressed by tumour cells and has been shown to enhance tumour angiogenesis and growth in preclinical models. We examined the expression of NRP-1 mRNA and EGF-R protein in seven human gastric cancer cell lines. NRP-1 expression was expressed in five of seven cell lines, and EGF-R expression closely mirrored NRP-1 expression. Moreover, in EGF-R-positive NCI-N87 and ST-2 cells, EGF induced both NRP-1 and VEGF mRNA expression. C225, a monoclonal antibody to EGF-R, blocked EGF-induced NRP-1 and VEGF expression in NCI-N87 cells in a dose-dependent manner. The treatment of NCI-N87 cells with EGF resulted in increases in phosphorylation of Erk1/2, Akt, and P38. Blockade of the Erk, phosphatidylinositol-3 kinase/Akt, or P38 pathways in this cell line prevented EGF induction of NRP-1 and VEGF. These results suggest that regulation of NRP-1 expression in human gastric cancer is intimately associated with the EGF/EGF-R system. Activation of EGF-R might contribute to gastric cancer angiogenesis by a mechanism that involves upregulation of VEGF and NRP-1 expression via multiple signalling pathways.

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“…Heregulin-a is expressed in ®broblasts associated with stomach tissue, but is capable of inducing proliferation of gastric cancer cells (Noguchi et al, 1999). Proliferation index, tumor invasion and metastasis frequency are higher for gastric cancer patients expressing both EGFR and EGF/TGF-a in cancer tissues (Borlinghaus et al, 1993;Filipe et al, 1995;Karameris et al, 1993;Yasui et al, 1988;Yonemura et al, 1991bYonemura et al, , 1992Yoshida et al, 1990). Although both EGF binding activity and EGFR immunoreactivity are important parameters for human gastric cancer progression, there is no signi®cant correlation between these two elements.…”

Section: Protein Localization By Immunohistochemistrymentioning

confidence: 99%

Tyrosine kinases and gastric cancer

et al. 2000

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Carcinoma of the stomach is one of the most prevalent cancer types in the world today. Two major forms of gastric cancer are distinguished according to their morphological and clinicopathological classi®cations (well dierentiated/intestinal type and poorly dierentiated/diuse type), characteristics that could also be attributed to the altered expression of dierent types of oncogenes or tumor suppressor genes. Signi®cant dierences exist for gastric cancer incidence comparing people of dierent ethnic origins, implicating various genetic and epigenetic factors for gastric oncogenesis. There are only a limited number of molecular markers available for gastric cancer detection and prognostic evaluation, among which are tyrosine kinases. There is convincing evidence that tyrosine kinases are involved in oncogenesis and disease progression for many human cancers. Ampli®cations of certain tyrosine kinases (cmet, k-sam and erbB2/neu) have been associated with human gastric cancer progression. Alternatively spliced transcripts and enhanced protein-expression levels for some of these tyrosine kinases are correlated with clinical outcomes for gastric cancer patients. With advent of high throughput techniques, it is now possible to detect nearly all expressed tyrosine kinases in a single screen. This increases the chance to identify additional tyrosine kinases as predictive markers for gastric cancers. In this article, we will ®rst review the literature data concerning certain tyrosine kinases implicated in gastric carcinogenesis and then summarize more recent work which provide comprehensive tyrosine kinase pro®les for gastric cancer specimens and cell lines. Two new gastric cancer molecular markers (tie-1 and mkk4) have been identi®ed through the use of these pro®les and demonstrated eective as clinical prognostic indicators. Oncogene (2000) 19, 5680 ± 5689.

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Expression of Epidermal Growth Factor, Transforming Growth Factor‐α and Their Receptor Genes in Human Gastric Carcinomas; Implication for Autocrine Growth

Cited by 91 publications

References 31 publications

Differential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts

Differential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts

Induction of neuropilin-1 and vascular endothelial growth factor by epidermal growth factor in human gastric cancer cells

Tyrosine kinases and gastric cancer

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