Expression of Enzymes Involved in Chlorophyll Catabolism in Arabidopsis Is Light Controlled

Banaś, Agnieszka Katarzyna; Łabuz, Justyna; Sztatelman, Olga; Gabryś, Halina; Fiedor, Leszek

doi:10.1104/pp.111.185504

Cited by 23 publications

(13 citation statements)

References 57 publications

(57 reference statements)

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“…In recent years, intensive efforts have been focused on deciphering the complex biological functions of Chlase and other crucial enzymes involved in chlorophyll turnover and maintaining its homeostasis at the physiological state of the cell, as well as during the adaptation of plants to a wide variety of biotic and abiotic stressors (Fernandez-Lopez et al, 1992;Karboune et al, 2005;Arkus & Jez, 2006;Azoulay-Shemer et al, 2008;Barry, 2009;Cowan, 2009;Beisel et al, 2010;Azoulay-Shemer et al, 2011;Banaś et al, 2011;Sytykiewicz et al, 2013). Surprisingly, numerous results regarding the participation of Chlase in senescence-induced chlorophyll diminution within different plant systems were often divergent or inconclusive (Wang et al, 2005;Ben-Yaakov et al, 2006;Criado et al, 2006;Hörtensteiner, 2006;Barry, 2009;Distefano et al, 2009;Gómez-Lobato et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Biochemical characterisation of chlorophyllase from leaves of selected Prunus species--a comparative study.

Sytykiewicz

Sprawka²,

Czerniewicz³

et al. 2013

Acta Biochim Pol

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Despite senescence-induced chlorophyll depletion in plants has been widely studied, the enzymatic background of this physiologically regulated process still remains highly unclear. The purpose of this study was to determine selected biochemical properties of partially purified fractions of chlorophyllase (Chlase, chlorophyll chlorophyllido-hydrolase, EC 3.1.1.14) from leaves of three Prunus species: bird cherry (Prunus padus L.), European plum (Prunus domestica L.), and sour cherry (Prunus cerasus L.). Secondarily, this report was aimed at comparing seasonal dynamics of Chlase activity and chlorophyll a (Chl a) content within investigated plant systems. Molecular weight of native Chlase F1 has been estimated at 90 kDa (bird cherry) and approximately 100 kDa (European plum and sour cherry), whereas molecular mass of Chlase F2 varied from 35 kDa (European plum) to 60 kDa (sour cherry). Furthermore, enzyme fractions possessed similar optimal pH values ranging from 7.6 to 8.0. It was found that among a broad panel of tested metal ions, Hg(+2), Fe(+2), and Cu(+2) cations showed the most pronounced inhibitory effect on the activity of Chlase. In contrast, the presence of Mg(+2) ions influenced a subtle stimulation of the enzymatic activity. Importantly, although Chlase activity was negatively correlated with the amount of Chl a in leaves of examined Prunus species, detailed comparative analyses revealed an incidental decrement of enzymatic activity in early or moderately senescing leaves. It provides evidence that foliar Chlase is not the only enzyme involved in autumnal chlorophyll breakdown and further in-depth studies elucidating this catabolic process are required.

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Section: Discussionmentioning

confidence: 99%

Biochemical characterisation of chlorophyllase from leaves of selected Prunus species--a comparative study.

Sytykiewicz

Sprawka²,

Czerniewicz³

et al. 2013

Acta Biochim Pol

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“…This finding is puzzling, because mature tomato cry1a plants grown in white light present a lower content of chlorophyll both in both leaves and fruits (Weller et al, 2001), and suggests that cryptochromes, and especially cry1a, can modulate chlorophyll accumulation in different plant organs. In Arabidopsis, blue light acting through cry1 upregulates the mRNA levels of chlorophyllase genes, involved in the chlorophyll catabolism (Bana s et al, 2011). This effect on chlorophyll content and the flowering acceleration, discussed below, recall typical shade-avoidance phenotypes (Cerdán and Chory, 2003;Cagnola et al, 2012), suggesting that cry1a/cry2 double mutant plants could be constitutive shade avoiders.…”

Section: Early Developmentmentioning

confidence: 90%

Pivotal Roles of Cryptochromes 1a and 2 in Tomato Development and Physiology

et al. 2018

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Cryptochromes are flavin-containing blue/UVA light photoreceptors that regulate various plant light-induced physiological processes. In Arabidopsis (Arabidopsis thaliana), cryptochromes mediate de-etiolation, photoperiodic control of flowering, entrainment of the circadian clock, cotyledon opening and expansion, anthocyanin accumulation, and root growth. In tomato (Solanum lycopersicum), cryptochromes are encoded by a multigene family, comprising CRY1a, CRY1b, CRY2, and CRY3. We have previously reported the phenotypes of tomato cry1a mutants and CRY2 overexpressing plants. Here, we report the isolation by targeting induced local lesions in genomes, of a tomato cry2 knockout mutant, its introgression in the indeterminate Moneymaker background, and the phenotypes of cry1a/cry2 single and double mutants. The cry1a/cry2 mutant showed phenotypes similar to its Arabidopsis counterpart (long hypocotyls in white and blue light), but also several additional features such as increased seed weight and internode length, enhanced hypocotyl length in red light, inhibited primary root growth under different light conditions, anticipation of flowering under long-day conditions, and alteration of the phase of circadian leaf movements. Both cry1a and cry2 control the levels of photosynthetic pigments in leaves, but cry2 has a predominant role in fruit pigmentation. Metabolites of the sterol, tocopherol, quinone, and sugar classes are differentially accumulated in cry1a and cry2 leaves and fruits. These results demonstrate a pivotal role of cryptochromes in controlling tomato development and physiology. The manipulation of these photoreceptors represents a powerful tool to influence important agronomic traits such as flowering time and fruit quality.

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“…Primers used for genotyping h1 mutants are listed in Supplemental Table S7. Double mutants phyAphyB and cry1cry2 were provided by Stanislaw Karpi nski (Banas et al, 2011). aba1 (Ler) was provided by Tomasz Sarnowski and Csaba Koncz (Strizhov et al, 1997).…”

Section: Plant Materialsmentioning

confidence: 99%

A specialized histone H1 variant is required for adaptive responses to complex abiotic stress and related DNA methylation in Arabidopsis

et al. 2015

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(A.J.).Linker (H1) histones play critical roles in chromatin compaction in higher eukaryotes. They are also the most variable of the histones, with numerous nonallelic variants cooccurring in the same cell. Plants contain a distinct subclass of minor H1 variants that are induced by drought and abscisic acid and have been implicated in mediating adaptive responses to stress. However, how these variants facilitate adaptation remains poorly understood. Here, we show that the single Arabidopsis (Arabidopsis thaliana) stressinducible variant H1.3 occurs in plants in two separate and most likely autonomous pools: a constitutive guard cell-specific pool and a facultative environmentally controlled pool localized in other tissues. Physiological and transcriptomic analyses of h1.3 null mutants demonstrate that H1.3 is required for both proper stomatal functioning under normal growth conditions and adaptive developmental responses to combined light and water deficiency. Using fluorescence recovery after photobleaching analysis, we show that H1.3 has superfast chromatin dynamics, and in contrast to the main Arabidopsis H1 variants H1.1 and H1.2, it has no stable bound fraction. The results of global occupancy studies demonstrate that, while H1.3 has the same overall binding properties as the main H1 variants, including predominant heterochromatin localization, it differs from them in its preferences for chromatin regions with epigenetic signatures of active and repressed transcription. We also show that H1.3 is required for a substantial part of DNA methylation associated with environmental stress, suggesting that the likely mechanism underlying H1.3 function may be the facilitation of chromatin accessibility by direct competition with the main H1 variants.Linker (H1) histones are conserved and ubiquitous structural components of eukaryotic chromatin required for the stabilization of higher order chromatin structure and are generally thought to restrict DNA accessibility. Interestingly, despite their architectural role, H1 histones were shown to be highly mobile and continuously exchanging among chromatin-binding sites (Raghuram et al., 2009). They are also the most variable of the histones, with numerous nonallelic variants coexisting in the same cell. In vertebrates, several evolutionarily conserved subfamilies of H1 can be distinguished (Talbert et al., 2012) and appear to play both redundant and specific roles during development and cellular differentiation (McBryant et al., 2010). There is accumulating evidence that, in animals, regulation of the proportions of H1 variants with different dynamic behavior in chromatin is involved in controlling the accessibility of DNA to trans-acting factors (Jullien et al., 2010;Shahhoseini et al., 2010;Zhang et al., 2012a;Pérez-Montero et al., 2013;Christophorou et al., 2014).Epigenetic mechanisms, including DNA and histone modifications and active nucleosome remodeling, are major players in translating signals about environmental perturbations into adaptive responses at the transc...

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Expression of Enzymes Involved in Chlorophyll Catabolism in Arabidopsis Is Light Controlled

Cited by 23 publications

References 57 publications

Biochemical characterisation of chlorophyllase from leaves of selected Prunus species--a comparative study.

Biochemical characterisation of chlorophyllase from leaves of selected Prunus species--a comparative study.

Pivotal Roles of Cryptochromes 1a and 2 in Tomato Development and Physiology

A specialized histone H1 variant is required for adaptive responses to complex abiotic stress and related DNA methylation in Arabidopsis

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