Expression of endothelial nitric oxide synthase in the uterus of patients with leiomyoma or adenomyosis

Oh, Na-Jung; Ryu, Ki Young; Jung, Cha-Nam; Yi, Sang Yeop; Kim, Se-Ryun

doi:10.1111/j.1447-0756.2012.01980.x

Cited by 15 publications

(16 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This increased local NO expression could cause activation of cyclooxygenase-2 and subsequently elevating the prostaglandins levels, such as prostaglandin E2 (34). On the other hand, the vascular expression of eNOS in our study, approves the findings by Gokdeniz et al, nevertheless their study mainly focuses on the muscular characteristics of myoma tissues (35) Although this study shows a non-significant increase in the expression of enzyme in vascular endothelium, this finding is not in contrast with results of Oh et al study (36) which found significant over-expression of enzyme in vasculature of the endothelium. Vascular over-expression of eNOS in case of uterine myoma may affect the biological integrity of endometrium.…”

Section: Discussionsupporting

confidence: 87%

Immunohistochemical Localization of Endothelial Nitric Oxide Synthase in Endometrial Tissues of Women With Uterine Myomas

Novin¹,

Moini²,

Niknezhad³

et al. 2018

International Journal of Women’s Health and Reproduction Scienc

View full text Add to dashboard Cite

Objectives: Nitric oxide (NO) is a free radical that plays important roles in variety of physiological aspects of the female reproductive system. Pathophysiological findings revealed a potential role of the endothelial isoform of nitric oxide synthase (eNOS) enzyme in female reproductive disorders specifically in the endometrium. This study investigates the expression of eNOS in the endometrial tissue to study the potential role of this enzyme and its NO production in infertility of women with uterine myomas. Materials and Methods: A total of 20 endometrial tissues were obtained, 10 infertile women with uterine fibroids and 10from normal and fertile women, 7 to 9 days after LH surge. Following fixation with paraformaldhyde, frozen sections of samples were prepared for semi-quantitative immunohistochemical evaluation using monoclonal anti-human eNOS antibody. Hematoxylin and eosin staining was used for histological dating of the samples Results: Localization of eNOS was seen in glandular and luminal epithelium, vascular endothelium and stroma in both fertile women and infertile women with uterine fibroids. Despite the differences in immunoreactivity of luminal epithelium, vascular endothelium and stroma in both groups, higher levels of eNOS in glandular epithelium was statistically significant in women with uterine fibroids compared to the control group. Conclusions: The findings suggest that over-expression of eNOS in glandular epithelium may affect the preparation stage of endometrium for fertility in women with uterine myomas.

show abstract

Section: Discussionsupporting

confidence: 87%

Immunohistochemical Localization of Endothelial Nitric Oxide Synthase in Endometrial Tissues of Women With Uterine Myomas

Novin¹,

Moini²,

Niknezhad³

et al. 2018

International Journal of Women’s Health and Reproduction Scienc

View full text Add to dashboard Cite

show abstract

“…iNOS expression is higher in leiomyoma than in normal myometrial cells 7,51 . Moreover, the uterus with leiomyoma or adenomyosis exhibited a higher expression of endothelial NOS, especially in cases associated with symptoms (menorrhagia and dysmenorrhea) 12 . This result illustrates that NOS may be involved in the pathomechanisms of invasion and excess growth of myometrium, similar to the process of vessel formation.…”

Section: Discussionmentioning

confidence: 96%

The Autonomic Innervation and Uterine Telocyte Interplay in Leiomyoma Formation

et al. 2019

View full text Add to dashboard Cite

The autonomic innervation of the uterus is involved in multiple pathophysiological processes in both humans and animals. Pathological conditions such as adenomyosis or inflammatory pelvic disease are usually accompanied by significant alterations in uterine innervation. In the current study, we focused on autonomic innervation of uterine fibroids, the identification of recently described interstitial cells, telocytes, and the possible interplay between these structures. In this work, uterine telocytes were identified by immunopositivity for c-kit, CD34, and PDGFRα. Nerves were revealed by immunolabeling for neuronal markers: protein gene product PGP 9.5, inducible nitric oxide synthase (iNOS), choline acetyltransferase (ChAT), and tyrosine hydroxylase (TH). The gross organization of myometrial tissue has been analyzed by routine histology. The results demonstrated that the density of iNOS and ChAT-immunopositive neurons in the uterine fibroids was higher than that in the control samples. The density of telocytes in the fibrosis foci was lower than that in the normal myometrium. Our results suggest that autonomic innervation and telocytes are involved in the microenvironment imbalance characteristic of uterine leiomyoma. Since NOS-positive nerves play an important role in oxidative stress modulation, they might lead to a decrease in the number of telocytes, which are crucial components in the pathogenesis of leiomyoma formation.

show abstract

“…In 2012, Aguilar-Melero P et al (Aguilar-Melero et al, 2012) indicated the significant association of NOS3 and cell survival in a hepatoma cell line. While Oh et al (2013) observed the expression of endothelial nitric oxide synthase was significantly higher in the uterus with leiomyoma or adenomyosis. When investigating the functions of the NOS3 gene in cancers, Terrazzino et al (2012) suggested NOS3 might be the marker for predicting acute skin toxicity in breast cancer patients receiving radiotherapy.…”

Section: Nitric Oxide Synthase 3 Gene Variants and Colorectal Cancer:mentioning

confidence: 72%

Nitric Oxide Synthase 3 Gene Variants and Colorectal Cancer: a Meta-Analysis

Yang

Li²,

Guo³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Expression of endothelial nitric oxide synthase in the uterus of patients with leiomyoma or adenomyosis

Cited by 15 publications

References 32 publications

Immunohistochemical Localization of Endothelial Nitric Oxide Synthase in Endometrial Tissues of Women With Uterine Myomas

Immunohistochemical Localization of Endothelial Nitric Oxide Synthase in Endometrial Tissues of Women With Uterine Myomas

The Autonomic Innervation and Uterine Telocyte Interplay in Leiomyoma Formation

Nitric Oxide Synthase 3 Gene Variants and Colorectal Cancer: a Meta-Analysis

Contact Info

Product

Resources

About