Expression of Drosophila Ca2+ permeable transient receptor potential-like channel protein in a prostate cancer cell line decreases cell survival

Zhang, Lei; Brereton, Helen M.; Hahn, Michael; Froscio, Mario; Tilley, Wayne D.; Brown, Michael P.; Barritt, Greg J.

doi:10.1038/sj.cgt.7700608

Cited by 9 publications

(12 citation statements)

References 48 publications

(60 reference statements)

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“…While it is an intriguing concept that a decline in inhibitory PIP 2 combined with a rise in H + might be the mechanism [43], a recent study argues that PUFAs gate TRPL [88], as proposed previously [47]. While there are many reports describing the biophysical properties of TRPL in heterologous expression system [89–94], until now there have been only three groups that have reported functional expression of TRP in vitro [90, 95, 96]. Apparently, this is due to difficulties in obtaining surface expression of TRP in expression systems.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 97%

Drosophila visual transduction

Montell

2012

Trends in Neurosciences

192

206

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Visual transduction in the Drosophila compound eye functions through a pathway that couples rhodopsin to phospholipase C (PLC) and the opening of Transient Receptor Potential (TRP) channels. This cascade differs from phototransduction in mammalian rods and cones, but is remarkably similar to signaling in mammalian intrinsically photosensitive retinal ganglion cells (ipRGCs). This review focuses on recent advances in the fly visual system, including the discovery of a visual cycle and insights into the machinery involved in generating a light response in photoreceptor cells. These latest findings suggest that the mechanism of light detection in flies and mammals have important parallels. Thus, a better understanding of phototransduction in the fly has the potential to contribute to our understanding of light detection in mammalian ipRGCs.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 97%

Drosophila visual transduction

Montell

2012

Trends in Neurosciences

192

206

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“…contributes to the initiation of necrosis by inducing cell swelling, the activation of proteases and phospholipids, and changes in membrane potential and ion transporters involved in volume regulation [87][88][89]. The strategy of using sustained elevated [Ca 2+ ] cyt to kill cells builds on earlier studies of Isaacs and others, which employed the SERCA inhibitor thapsigargin to release Ca 2+ from the ER which, in turn, induces ER stress and activates sustained Ca 2+ entry through SOCs [90].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

TRP channels in cancer

Prevarskaya

Zhang²,

Barritt

2007

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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281

201

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The progression of cells from a normal differentiated state in which rates of proliferation and apoptosis are balanced to a tumorigenic and metastatic state involves the accumulation of mutations in multiple key signalling proteins and the evolution and clonal selection of more aggressive cell phenotypes. These events are associated with changes in the expression of numerous other proteins. This process of tumorigenesis involves the altered expression of one or more TRP proteins, depending on the nature of the cancer. The most clearly described changes are those involving TRPM8, TRPV6 and TRPM1. Expression of TRPM8 is substantially increased in androgen-dependent prostate cancer cells, but is decreased in androgen independent and metastatic prostate cancer. TRPM8 expression is regulated, in part, by androgens, most likely through androgen response elements in the TRPM8 promoter region. TRPM8 channels are involved in the regulation of cell proliferation and apoptosis. Expression of TRPV6 is also increased in prostate cancer and in a number of other cancers. In contrast to TRPM8, expression of TRPV6 is not directly regulated by androgens. TRPM1 is highly expressed in early stage melanomas but its expression declines with increases in the degree of aggressiveness of the melanoma. The expression of TRPV1, TRPC1, TRPC6, TRPM4, and TRPM5 is also increased in some cancers. The level of expression of TRPM8 and TRPV6 in prostate cancer, and of TRPM1 in melanomas, potentially provides a good prognostic marker for predicting the course of the cancer in individuals. The Drosophila melanogaster, TRPL, and the TRPV1 and TRPM8 proteins, have been used to try to develop strategies to selectively kill cancer cells by activating Ca(2+) and Na(+) entry, producing a sustained increase in the cytoplasmic concentration of these ions, and subsequent cell death by apoptosis and necrosis. TRPV1 is expressed in neurones involved in sensing cancer pain, and is a potential target for pharmacological inhibition of cancer pain in bone metastases, pancreatic cancer and most likely in other cancers. Further studies are required to assess which other TRP proteins are associated with the development and progression of cancer, what roles TRP proteins play in this process, and to develop further knowledge of TRP proteins as targets for pharmaceutical intervention and targeting in cancer.

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“…2B). An antibody against CD-9, located at the PM in LNCaP cells and in a number of other cell types (8,10), was used as a PM marker. Images of LNCaP cells treated with the anti-CD-9 antibody revealed a clear location of the CD-9 protein at the PM (Fig.…”

Section: Detection Of Trpm8 Expression and Itsmentioning

confidence: 99%

“…Intracellular Ca 2ϩ imaging using Fura-2 was conducted as described previously (8). Temperature was controlled by a temperature controller (Cell Microcontrol System, Norfolk, VA) and monitored by a thermistor placed within 160 m of the field of view.…”

Section: Introductionmentioning

confidence: 99%

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Evidence that TRPM8 Is an Androgen-Dependent Ca2+ Channel Required for the Survival of Prostate Cancer Cells

2004

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The Ca 2؉ -permeable channel TRPM8 is thought to play an important role in the pathophysiology of prostate cancer. We have investigated the intracellular location of TRPM8 and its role as a Ca 2؉ -permeable channel in an androgen-responsive and an androgen-insensitive prostate cancer cell line. We report evidence from immunofluorescence experiments that in the androgen-responsive LNCaP cell line, the TRPM8 protein is expressed in the endoplasmic reticulum and plasma membrane, acts as a Ca 2؉ -permeable channel (assessed using Fura-2 to measure increases in the cytoplasmic Ca 2؉ concentration) in each of these membranes, and is regulated by androgen. Although TRPM8 was detected in the androgeninsensitive PC-3 cell line, no evidence was obtained for regulation of its expression by androgen. The results of experiments using LNCaP cells, the TRPM8 antagonist capsazepine, and small interference RNA targeted to TRPM8 indicate that TRPM8 is required for cell survival. These results indicate that TRPM8 is an important determinator of Ca 2؉ homeostasis in prostate epithelial cells and may be a potential target for the action of drugs in the management of prostate cancer.

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Expression of Drosophila Ca2+ permeable transient receptor potential-like channel protein in a prostate cancer cell line decreases cell survival

Cited by 9 publications

References 48 publications

Drosophila visual transduction

Drosophila visual transduction

TRP channels in cancer

Evidence that TRPM8 Is an Androgen-Dependent Ca2+ Channel Required for the Survival of Prostate Cancer Cells

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