Expression of dipeptidyl aminopeptidase IV activity in thyroid carcinoma

Kotani, Takuya; Aratake, Yatsuki; Ogata, Yoshitake; Umeki, Kazumi; Araki, Yasunori; Hirai, Keisuke; Kuma, Kanji; Ohtaki, Sachiya

doi:10.1016/0304-3835(91)90158-e

Cited by 38 publications

(31 citation statements)

References 19 publications

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“…The frequency of DPPIV/CD26 mRNA overexpression in differentiated thyroid carcinomas found in this study coincided with that in our previous histochemical results [8]. In DPPIV/CD26 activity staining analysis, all benign tissues were DPPIV/ CD26 negative, while all differentiated thyroid carcinomas showed strong DPPIV/CD26 activity.…”

Section: Discussionsupporting

confidence: 91%

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Differential Expression of Dipeptidyl Peptidase IV (CD26) and Thyroid Peroxidase in Neoplastic Thyroid Tissues.

et al. 1996

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Section: Discussionsupporting

confidence: 91%

“…TPO activity in thyroid carcinoma tissues was greatly reduced [6,7], and increased DPPIV/CD26 activity was observed in nearly 100% of differentiated thyroid carcinomas which account for more than 95% of all thyroid cancer cases [8]. These two enzymes can be detected histochemically on tissue sections.…”

mentioning

confidence: 94%

Differential Expression of Dipeptidyl Peptidase IV (CD26) and Thyroid Peroxidase in Neoplastic Thyroid Tissues.

et al. 1996

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“…9,16 We have reported that thyroid carcinoma expresses aberrant dipeptidyl peptidase IV (CD26/DPPIV) and that enzymatic and immunohistochemical stainings of CD26/DPPIV are useful for the pathological and cytological diagnosis of thyroid tumors. [17][18][19][20] Our observations on CD26/DPPIV overexpression in thyroid neoplasms have been supported by many researchers. [21][22][23] CD26/DPPIV is a membranebound enzyme that hydrolyzes the N-terminal-end of proline-containing peptides.…”

supporting

confidence: 63%

Diagnostic utility of galectin‐3 and CD26/DPPIV as preoperative diagnostic markers for thyroid nodules

Aratake

Umeki

Kiyoyama

et al. 2002

Diagnostic Cytopathology

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The aim of this study was to search for diagnostic markers that could correctly identify thyroid nodular lesions requiring urgent surgical treatment. We investigated whether galectin-3 and dipeptidyl peptidase IV (CD26/DPPIV) could be potential markers for improving the diagnostic accuracy of conventional cytology. Seventy-nine patients with histologically proven thyroid diseases were analyzed. The immunocytochemical staining results showed galectin-3 expression in neoplastic cells of all 37 papillary carcinomas, five of six follicular carcinomas, all three anaplastic carcinomas, one of three medullary carcinomas, and two of 14 follicular adenomas. All 16 adenomatous goiters were negative for galectin-3 immunostaining. On the other hand, all 37 papillary carcinomas, all six follicular carcinomas, and one of three anaplastic carcinomas revealed CD26/DPPIV expression, whereas all three medullary carcinomas were negative. Among benign thyroid lesions, four of 14 follicular adenomas and two of 16 adenomatous goiters exhibited varying degrees of immunoreactivity for CD26/DPPIV. RT-PCR analysis demonstrated overexpression of galectin-3 and CD26/DPPIV mRNAs in all six papillary and all three follicular carcinomas analyzed, whereas the mRNA expressions of these molecules were barely or not detectable in benign thyroid lesions and normal thyroid tissues, except for one case of follicular adenoma. In conclusion, we demonstrate that galectin-3 and CD26/DPPIV were consistently coexpressed at protein and mRNA levels in differentiated thyroid carcinomas. We propose that combined immunostaining for galectin-3 and CD26/DPPIV in the preoperative evaluation of thyroid nodules may play a role in accurate cytodiagnosis.

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“…1, 15 Aratake et al reported that all papillary or follicular carcinomas showed strong positive staining, while most benign follicular adenoma or adenomatous goiter cases were negative. 1 Our results also show that all papillary carcinomas were positive and most adenomatous goiter cases negative.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, dipeptidyl aminopeptidase IV (DAP IV) activity staining has been found to be elevated in thyroid cancers, and so DAP IV activity may be a good index of malignancy in thyroid disease. 1,15 DAP IV is the enzyme bound to the cellular membrane and is known to hydrolyze the N-terminal end of proline-containing peptides. 12,13,17 This enzyme is found extensively throughout the body, es-pecially in the liver, kidney and salivary glands.…”

mentioning

confidence: 99%