Expression of cytoskeleton-associated protein 4 is related to lymphatic metastasis and indicates prognosis of intrahepatic cholangiocarcinoma patients after surgery resection

Li, Minhong; Dong, Lixue; Li, Shuangxi; Tang, Gusheng; Pan, Yu-Fei; Zhang, Jian; Wang, Hui; Zhou, Hai-Hua; Tan, Ye-Xiong; Hu, He-Ping; Wang, Hongyang

doi:10.1016/j.canlet.2013.05.003

Cited by 27 publications

(21 citation statements)

References 25 publications

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“…All four datasets showed consistent results, indicating that CKAP4 mRNA levels were significantly higher in tumor tissues compared with adjacent liver tissues. These results confirm the upregulation of CKAP4 in tumor tissues in accordance with previous results found in HCC (19) and other types of cancer, including cervical cancer, bladder cancer, lung cancer, pancreatic cancer, ESCC, CLL, PDAC and intrahepatic cholangiocellular carcinoma (12)(13)(14)(15)(16)(17)(18)27).…”

Section: Discussionsupporting

confidence: 92%

Cytoskeleton-associated membrane protein�4 is upregulated in tumor tissues and is associated with clinicopathological characteristics and prognosis in hepatocellular carcinoma

et al. 2020

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The role of cytoskeleton-associated membrane protein 4 (CKAP4) in hepatocellular carcinoma (HCC) is controversial. The present study aimed to investigate the association between tumor CKAP4 mRNA expression and clinicopathological characteristics and prognosis in patients with HCC. Data relating to CKAP4 mRNA expression in HCC tumor and normal adjacent liver tissues, and clinicopathological characteristics, were downloaded from the Gene Expression Omnibus and The Cancer Genome Atlas databases. The CKAP4 mRNA levels in tumor tissues were compared with those in normal adjacent liver tissues, their association with clinicopathological parameters was analyzed, and diagnostic and prognostic values were evaluated in patients with HCC. In all 4 datasets (total samples, n=693), CKAP4 mRNA levels were significantly higher in tumor tissues compared with adjacent tissues (all P<0.001), with the area under the receiver operating characteristic curve ranging from 0.799-0.898 for HCC diagnosis. In patients with HCC with available clinical data (n=361), the low-level CKAP4 mRNA group exhibited a lower body mass index (P=0.005), higher α-fetoprotein level (P<0.001), more frequent adjacent liver tissue inflammation (P<0.001), poorer tumor histological grade (P<0.001), higher Ishak fibrosis score (P= 0.035) and a more advanced tumor node metastasis (TNM) stage (P=0.014) compared with the high-level CKAP4 mRNA group. Patients stratified by all the above parameters, except for TNM stage, exhibited significantly different expression of tissue CKAP4 mRNA (P<0.05-0.001). Furthermore, higher CKAP4 mRNA levels were observed in patients who died within one year following diagnosis compared with those who survived >3 years (P=0.003). The high-level CKAP4 mRNA group also exhibited lower overall survival (OS) and disease-free survival (DFS) rates compared with the low-level group [hazard ratio (HR)=1.494; 95% confidence interval (CI), 1.044-2.138; P=0.028] for OS and (HR=1.616; 95% CI, 1.022-2.555; P=0.040) for DFS. The results of the present study suggest that CKAP4 mRNA is upregulated in HCC tumor tissues compared with normal adjacent tissues, and is associated with poor clinical prognosis, pathological features and survival in patients with HCC. Thus, CKAP4 is a potential biomarker for HCC diagnosis and prognosis.

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Section: Discussionsupporting

confidence: 92%

Cytoskeleton-associated membrane protein�4 is upregulated in tumor tissues and is associated with clinicopathological characteristics and prognosis in hepatocellular carcinoma

et al. 2020

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“…It was reported that CKAP4 plays important roles in several cellular processes, including endoplasmic reticulum anchoring 18 and morphology determination 19, regulation of Dicer function 20, cell proliferation 21 and tumour migration 22, 23. Knockdown of CKAP4 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Transmembrane domain is crucial to the subcellular localization and function of Myc target 1

Gui

Yin

et al. 2015

J Cellular Molecular Medi

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Deregulation of c‐MYC occurs in a variety of human cancers. Overexpression of c‐MYC promotes cell growth, proliferation, apoptosis, transformation and genomic instability. MYC target 1 (MYCT1) is a direct target gene of c‐MYC, and its murine homologue MT‐MC1 recapitulated multiple c‐Myc‐related phenotypes. However, the molecular mechanism of MYCT1 remains unclear. Here, we identified the transmembrane (TM) domain of MYCT1, not the nuclear localization sequence, is indispensable to the vesicle‐associated localization of MYCT1 protein in the cytoplasmic membrane vesicle. Overexpression of MYCT1, not MYCT1 (ΔTM), decreased cell viability under serum deprivation and increased tumour cell migration ability. We further identified CKAP4 interacted with MYCT1 and contributed to the function of MYCT1. In addition, we found that a mutation, A88D, which is observed in patient sample, changed the localization, and abolished the effect on cell viability and cell migration, suggesting that the TM domain is critical to MYCT1.

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“…Stained sections were evaluated in a blinded manner without prior knowledge of the clinical data using the German immunoreactive score (IRS), as described previously [ 20 , 21 ]. Briefly, staining intensity was graded as “0” (negative), “1” (weak), “2” (moderate) and “3” (strong); staining extent was graded as “0” (<5%), “1” (5-25%), “2” (25-50%), “3” (50-75%) and “4” (>75%).…”

Section: Methodsmentioning

confidence: 99%

Serine threonine tyrosine kinase 1 is a potential prognostic marker in colorectal cancer

Chen

Cai

et al. 2015

BMC Cancer

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BackgroundAberrant expression of serine threonine tyrosine kinase 1 (STYK1) has been reported in several human malignancies including colorectal cancer (CRC). However, the prognostic significance of STYK1 expression in CRC remains unknown.MethodsSTYK1 protein expression in paraffin-embedded CRC specimens was determined immunohistochemically. The correlation of STYK1 expression with clinicopathologic features was assessed in a cohort containing 353 patients with primary CRC. Kaplan-Meier and Cox proportional regression analyses were used to evaluate the association between STYK1 expression and patients’ survival.ResultsSTYK1 expression was frequently up-regulated in CRC clinical samples at the protein levels and was significantly associated with tumor differentiation grade (p = 0.030), lymph node metastasis (p = 0.004), TNM stage (p = 0.007) and patient death (p < 0.001). Kaplan-Meier analysis indicated that patients with high intratumoral STYK1 expression had a significantly shorter disease-specific survival (DSS) than those with low expression (p < 0.001). Importantly, high levels of STYK1 protein predicted poor DSS for both stage II (p < 0.001) and stage III (p = 0.004) patients. Furthermore, multivariate analyses revealed that STYK1 protein expression was an independent prognostic indicator for both stage II (hazard ratio [HR], 2.472; p = 0.001) and stage III (HR, 2.001; p = 0.004) patients.ConclusionsOur results suggest that increased STYK1 protein expression correlates with disease progression and metastasis and may serve as a predictor of poor survival in CRC.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-015-1285-y) contains supplementary material, which is available to authorized users.

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Expression of cytoskeleton-associated protein 4 is related to lymphatic metastasis and indicates prognosis of intrahepatic cholangiocarcinoma patients after surgery resection

Cited by 27 publications

References 25 publications

Cytoskeleton-associated membrane protein�4 is upregulated in tumor tissues and is associated with clinicopathological characteristics and prognosis in hepatocellular carcinoma

Cytoskeleton-associated membrane protein�4 is upregulated in tumor tissues and is associated with clinicopathological characteristics and prognosis in hepatocellular carcinoma

Transmembrane domain is crucial to the subcellular localization and function of Myc target 1

Serine threonine tyrosine kinase 1 is a potential prognostic marker in colorectal cancer

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