Expression of Cytokine- and Chemokine-Related Genes in Peripheral Blood Mononuclear Cells from Lupus Patients by cDNA Array

Rus, Violeta; Atamas, Sergei P.; Shustova, Valentina; Luzina, Irina G.; Selaru, Florin M.; Magder, Laurence S.; Via, Charles S.

doi:10.1006/clim.2001.5182

Cited by 96 publications

(85 citation statements)

References 40 publications

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“…Most of the genes identified in this study as differentially expressed, such as IL-1RII, MMP9, CCR7, IL-18R, IL1RN, showed expression patterns similar to those reported previously using membrane microarrays. 17 Other results reported previously in SLE patients, such as increased IL-10, IL-6, and bcl-2, were not confirmed by our microarray data. 17 The fluorescent intensity of these genes was equal to the background fluorescence intensity.…”

Section: Discussioncontrasting

confidence: 92%

“…17 Other results reported previously in SLE patients, such as increased IL-10, IL-6, and bcl-2, were not confirmed by our microarray data. 17 The fluorescent intensity of these genes was equal to the background fluorescence intensity. Therefore, we suspect that such false-negative results were due to the relatively low sensitivity of the oligonucleotide microarray method, resulting in a bias of the array data toward abundant transcripts.…”

Section: Discussioncontrasting

confidence: 92%

“…17 Hierarchical clustering showed that patient samples were clearly separated from controls based on their differential gene expression profile. These differential expression genes can be taken as characteristic signatures of gene expression in SLE patients for clinical diagnosis and differential diagnosis.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of gene expression profiles in human systemic lupus erythematosus using oligonucleotide microarray

et al. 2003

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Epidemiologic studies suggest a strong genetic component for susceptibility to systemic lupus erythematosus (SLE). To investigate the genetic mechanism of pathogenesis of SLE, we studied the difference in gene expression of peripheral blood cells between 10 SLE patients and 18 healthy controls using oligonucleotide microarray. When gene expression for patients was compared to the mean of normal controls, among the 3002 target genes, 61 genes were identified with greater than a twofold change difference in expression level. Of these genes, 24 were upregulated and 37 downregulated in at least half of the patients. By the Welch's ANOVA/Welch's t-test, all these 61 genes were significantly different (Po0.05) between SLE patients and normal controls. Among these genes with differential expression, IFN-o and Ly6E (TSA-1/Sca-2) may play an important role in the mechanism of SLE pathogenesis. TSA-1 antigens may represent an important alternative pathway for T-cell activation that may be involved in IFN-mediated immunomodulation. Hierarchical clustering showed that patient samples were clearly separated from controls based on their gene expression profile. These results demonstrate that high-density oligonucleotide microarray has the potential to explore the mechanism of pathogenesis of systemic lupus erythematosus.

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Section: Discussioncontrasting

confidence: 92%

Section: Discussioncontrasting

confidence: 92%

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Analysis of gene expression profiles in human systemic lupus erythematosus using oligonucleotide microarray

et al. 2003

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“…Diseases characterized by inflammation and by activated immune responses are obvious targets for initial experiments, and indeed a number of groups, including our own, have initiated such studies. 3,5,18,19 In order for the information from peripheral blood cell gene expression to be optimally useful for translational clinical studies, it will be important to attempt to control as many variables as possible. Many factors are known to dynamically influence the numbers and function of peripheral blood cells and the relative proportions of cells at a given point in time.…”

Section: Discussionmentioning

confidence: 99%

Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation

et al. 2004

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“…Recently, microarray analyses has been used by other investigators to study differential gene expression patterns in SLE patients as compared with healthy controls. [32][33][34][35][36][37] In this study, we investigated differential gene expression in the splenic cells of pConstolerized BWF1 mice to cells from unmanipulated BWF1 mice to better understand the molecular mechanisms of suppression/tolerance. We investigated genes that are differentially expressed after pCons treatment in BWF1 mice in splenic white blood cells (WBC), CD4 þ T cells and CD8 þ T cells.…”

Section: Introductionmentioning

confidence: 99%

Distinct gene signature revealed in white blood cells, CD4+ and CD8+ T cells in (NZBx NZW) F1 lupus mice after tolerization with anti-DNA Ig peptide

et al. 2010

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Tolerizing mice polygenically predisposed to lupus-like disease (NZB/NZW F1 females) with a peptide mimicking anti-DNA IgG sequences containing MHC class I and class II T cell determinants (pConsensus, pCons) results in protection from full-blown disease attributable in part to the induction of CD4 þ CD25 þ Foxp3 þ and CD8 þ Foxp3 þ regulatory T cells. We compared 45 000 murine genes in total white blood cells (WBC), CD4 þ T cells, and CD8 þ T cells from splenocytes of (NZBxNZW) F1 lupus-prone mice tolerized with pCons vs untreated naïve mice and found two-fold or greater differential expression for 448 WBC, 174 CD4, and 60 CD8 genes. We identified differentially expressed genes that played roles in the immune response and apoptosis. Using real-time PCR, we validated differential expression of selected genes (IFI202B, Bcl2, Foxp3, Trp-53, CCR7 and IFNar1) in the CD8 þ T cell microarray and determined expression of selected highly upregulated genes in different immune cell subsets. We also determined Smads expression in different immune cell subsets, including CD4 þ T cells and CD8 þ T cells, to detect the effects of TGF-b, known to be the major cytokine that accounts for the suppressive capacity of CD8 þ Treg in this system. Silencing of anti-apoptotic gene Bcl2 or interferon genes (IFI202b and IFNar1 in combination) in CD8 þ T cells from tolerized mice did not affect the expression of the other selected genes. However, silencing of Foxp3 reduced expression of Foxp3, Ifi202b and PD1-all of which are involved in the suppressive capacity of CD8 þ Treg in this model.

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Expression of Cytokine- and Chemokine-Related Genes in Peripheral Blood Mononuclear Cells from Lupus Patients by cDNA Array

Cited by 96 publications

References 40 publications

Analysis of gene expression profiles in human systemic lupus erythematosus using oligonucleotide microarray

Analysis of gene expression profiles in human systemic lupus erythematosus using oligonucleotide microarray

Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation

Distinct gene signature revealed in white blood cells, CD4+ and CD8+ T cells in (NZBx NZW) F1 lupus mice after tolerization with anti-DNA Ig peptide

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