Expression of CYP4 and GSTr genes in Venerupis philippinarum exposed to benzo(a)pyrene

Papo, Michele Boscolo; Maccatrozzo, Lisa; Bertotto, Daniela; Pascoli, Francesco; Negrato, Elena; Poltronieri, Carlo; Binato, Giovanni; Gallina, Albino; Radaelli, Giuseppe

doi:10.1016/j.aanat.2013.11.003

Cited by 9 publications

(1 citation statement)

References 48 publications

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“…Total purified RNA (by TRIZOL Reagent from Invitrogen, Thermo Fisher Scientific) was quantified using NanoDrop Spectrophotometers (Thermo Fisher Scientific) and RNA quality was evaluated by gel electrophoresis according to Boscolo Papo et al (2014). The cDNA was synthesized from 1 μg of the total RNA using the High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems, Thermo Fisher Scientific), in accordance to the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

Neuromuscular Electrical Stimulation Induces Skeletal Muscle Fiber Remodeling and Specific Gene Expression Profile in Healthy Elderly

et al. 2019

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Skeletal muscle aging is a multifactorial process strictly related to progressive weakness. One of the results that were focused on was the fiber phenotype modification and their loss. The physiological muscle recruitment to contraction, basically prosecuted under volitional control, can also be engaged by means of Neuromuscular Electrical Stimulation (NMES). Knowing that the NMES is effective in improving muscle strength in active healthy elderly, the aim was to investigate which physiological modifications were able to produce in the Vastus lateralis muscle and the pathways involved. It was found that NMES increased the cross sectional area and the isometric strength of type II myofibers together with the activated myogenic pathway in order to shift glycolytic toward the oxidative phenotype II myofibers, at a molecular level and with an increase of maximal voluntary contraction (MVC) at a functional level. Using the TaqMan low density array on 48 different genes, we found that NMES specific gene regulation highlighted: (i) increased protein synthesis with respect to protein degradation; (ii) the activation of an apoptotic pathway involved in the differentiation process; (iii) increased regeneration signals; (iv) oxidative enzyme regulation. These pathways were validated via confirmatory RT-PCR for genes involved in the regeneration process as well as Myosin isoforms. We also investigated the oxidative stress status analyzing superoxide anion levels, the protein expression of two different superoxide dismutase and the activity of both catalase and superoxide anion dismutase, being two main antioxidant enzymes. In conclusion, data demonstrates that NMES is effective in producing physiological adaptation on Vastus Lateralis of active healthy elderly as well as providing new insights for further research on elderly who experienced muscle detriment for periodic or permanent immobility.

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Section: Methodsmentioning

confidence: 99%

Neuromuscular Electrical Stimulation Induces Skeletal Muscle Fiber Remodeling and Specific Gene Expression Profile in Healthy Elderly

et al. 2019

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Characterization of the molecular differential responses in marine benthic macroinvertebrates exposed to polycyclic aromatic hydrocarbons

Onyena,

Manohar,

Nkwoji

et al. 2023

Aquat Ecol

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Gene cloning and expression analysis of AhR and CYP4 from Pinctada martensii after exposed to pyrene

Liao

Zhou

et al. 2015

Ecotoxicology

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Pyrene, a typical polycyclic aromatic hydrocarbon, is a common pollutant in the marine environment. Polycyclic aromatic hydrocarbons initiate cellular detoxification in an exposed organism via the activation of the aryl hydrocarbon receptor (AhR). Subsequent metabolism of these xenobiotics is mainly by the cytochrome P450 enzymes of the phase I detoxification system. Full-length complementary DNA sequences from the pearl oyster Pinctada martensii (pm) encoding AhR and cytochrome P4 were cloned. The P. martensii AhR complementary DNA sequence constitutes an open reading frame that encodes for 848 amino acids. Sequence analysis indicated PmAhR showed high similarity with its homologues of other bivalve species. The cytochrome P(CYP)4 complementary DNA sequence of P. martensii constitutes an open reading frame that encodes for 489 amino acids. Quantitative real-time analysis detected both PmAhR and PmCYP4 messenger RNA expressions in the mantle, gill, hepatapancreas and adductor muscle of P. martensii exposed to pyrene. The highest transcript-band intensities of PmAhR and PmCYP4 were observed in the gill. Temporal expression of PmAhR and PmCYP4 messenger RNAs induction was observed in gills and increased between 3 and 5 days post exposure; then returned to control level. These results suggest that messenger RNAs of PmAhR and PmCYP4 in pearl oysters might be useful parameters for monitoring marine environment pyrene pollution.

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Expression of CYP4 and GSTr genes in Venerupis philippinarum exposed to benzo(a)pyrene

Cited by 9 publications

References 48 publications

Neuromuscular Electrical Stimulation Induces Skeletal Muscle Fiber Remodeling and Specific Gene Expression Profile in Healthy Elderly

Neuromuscular Electrical Stimulation Induces Skeletal Muscle Fiber Remodeling and Specific Gene Expression Profile in Healthy Elderly

Characterization of the molecular differential responses in marine benthic macroinvertebrates exposed to polycyclic aromatic hydrocarbons

Gene cloning and expression analysis of AhR and CYP4 from Pinctada martensii after exposed to pyrene

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