Expression of Cyclin-D1 and p53 as Prognostic Markers in Treatment of Oral Squamous Cell Carcinoma

Kakkar, Vikas; Sarin, Vanita; Chatterjee, Arpita; Manjari, Mridu; Chopra, Ishita

doi:10.1007/s12070-021-02716-4

Cited by 4 publications

(4 citation statements)

References 41 publications

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“…Most studies in literature have used only IHC on paraffin blocks and correlated the results with nodal metastases, lymphovascular invasion, and other pathological variables. [11][12][13] Studies have reported variable overexpression of cyclin D1 in oral squamous cell cancers ranging from 18 to 56%, 14 and the same was seen in our results with the IHC being positive in 68.3% of the patients, whereas the Qf PCR was positive for cyclin D1 in only 36.6%. This brings to view an important fact that even if the copy number is not altered, in approximately 32% of these patients there is functional overexpression of cyclin D1.…”

Section: Discussionsupporting

confidence: 86%

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Copy Number Alteration of Cyclin D1 (CCND 1) Gene as a Prognostic Factor in Oral Squamous Cancers and its Correlation with Immunohistochemistry (IHC)

Ratnagiri,

Jena,

Ranganath

et al. 2024

South Asian J Cancer

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Background Oral squamous cancers remain the most common cancers among males in India and the third most common cancer among men and women combined, with an age standardized ratio of 9.1 per 100,000 population. Surgery and chemoradiation have not improved survival rates significantly and hence, newer therapeutic targets are needed. Cyclin D1 (CCND1) is a proto-oncogene which is located on chromosome 11q13 and has been found to be amplified in many cancers including oral cancers. It is said to correlate with aggressive tumor growth and a poorer prognosis. Aim This article correlates cyclin D1 (CCND1) gene copy number alteration with clinicopathologic prognostic factors in oral squamous cancers. Materials and Methods Sixty-three patients who underwent surgery for oral cancer between January and June 2022 were included in the study after obtaining informed consent and ethics approval. Copy number alteration of CCND1 was assessed using quantitative polymerase chain reaction (Qf PCR) and correlated with clinical and histopathological prognostic factors, including short-term recurrence. Statistical analysis was performed using the SPSS software. Results A statistically significant correlation was determined between the Qf PCR values of CCND1 gene and locoregional recurrence. Gene copy number alteration also correlated strongly with a higher grade of the primary tumor. There was also a significant correlation between the Qf PCR values and the immunohistochemistry for cyclin D1. Conclusion Cyclin D1 offers a new therapeutic target in oral cancers and may improve survival without significant treatment-related morbidity.

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Section: Discussionsupporting

confidence: 86%

“…Most studies in literature have used only IHC on paraffin blocks and correlated the results with nodal metastases, lymphovascular invasion, and other pathological variables. 11 12 13…”

Section: Discussionmentioning

confidence: 99%

Copy Number Alteration of Cyclin D1 (CCND 1) Gene as a Prognostic Factor in Oral Squamous Cancers and its Correlation with Immunohistochemistry (IHC)

Ratnagiri,

Jena,

Ranganath

et al. 2024

South Asian J Cancer

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“…In accordance with these findings. Kakkar et al [ 15 ] in a study involving 100 cases of oral SCC concluded that high p53 expression was associated with poor overall survival and disease-free survival in HNSCC.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological Parameters and Biomarker Profile in a Cohort of Patients With Head and Neck Squamous Cell Carcinoma (HNSCC)

Hashmi,

Bukhari,

Aslam

et al. 2023

Cureus

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Introduction: Squamous cell carcinoma (SCC) is the most common malignancy of the head and neck region, commonly termed as head and neck squamous cell carcinoma (HNSCC). Data related to biomarker expression in HNSCC are scarcely available, especially in our population. This study aimed to evaluate the association of immunohistochemical (IHC) expression of p16, epidermal growth factor receptor (EGFR), p27, and p53 in HNSCC with clinical and pathological parameters.

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“…EGFR ( 35 – 38 ), p53 ( 39 – 41 ), Ki67 ( 29 , 42 – 48 ), already available and consolidated in their diagnostic effectiveness of OSCC;…”

Section: Methodsmentioning

confidence: 99%

A new method for oral cancer biomarkers detection with a non-invasive cyto-salivary sampling and rapid-highly sensitive ELISA immunoassay: a pilot study in humans

et al. 2023

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IntroductionOral squamous cell carcinoma (OSCC) accounts for approximately 90% of oral malignancies and has a 5-year mortality rate close to 50%. A consistent part (70%) of all oral cancers is diagnosed at an advanced stage since available screening techniques are ineffective. Therefore, it would be urgent to improve them. The diagnostic gold standard is tissue biopsy with histological and immunohistochemical assessment. This method presents some limitations. Biopsy is invasive and the histopathological evaluation is semi-quantitative, and the absolute abundance of the target cannot be reliably determined. In addition, tissue is highly processed and may lead to loss of information of the natural state. The search for classical and new clinical biomarkers on fragments of tissue/cells collected with a cytobrush is a highly hopeful technique for early detection and diagnosis of OSCC, because of its non-invasive sampling and easy collection method.MethodsHere we analyzed cytobrush biopsies samples collected from the oral cavity of 15 patients with already diagnosed OSCC by applying an innovative high-sensitivity ELISA technique, in order to verify if this approach may provide useful information for detection, diagnosis, and prognosis of OSCC. To this end, we selected six biomarkers, already used in clinical practice for the diagnosis of OSCC (EGFR, Ki67, p53) or selected based on recent scientific and clinical data which indicate their presence or over-expression in cells undergoing transformation and their role as possible molecular targets in immunecheckpoints blockade therapies (PD-L1, HLA-E, B7-H6).ResultsThe selected tumor biomarkers were highly expressed in the tumor core, while were virtually negative in healthy tissue collected from the same patients. These differences were highly statistically significant and consistent with those obtained using the gold standard test clearly indicating that the proposed approach, i.e. analysis of biomarkers by a custom ELISA technique, is strongly reliable.DiscussionThese preliminary data suggest that this non-invasive rapid phenotyping technique could be useful as a screening tool for phenotyping oral lesions and support clinical practice by precise indications on the characteristics of the lesion, also with a view to the application of new anti-tumor treatments, such as immunotherapy, aimed at OSCC patients.

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Expression of Cyclin-D1 and p53 as Prognostic Markers in Treatment of Oral Squamous Cell Carcinoma

Cited by 4 publications

References 41 publications

Copy Number Alteration of Cyclin D1 (CCND 1) Gene as a Prognostic Factor in Oral Squamous Cancers and its Correlation with Immunohistochemistry (IHC)

Copy Number Alteration of Cyclin D1 (CCND 1) Gene as a Prognostic Factor in Oral Squamous Cancers and its Correlation with Immunohistochemistry (IHC)

Clinicopathological Parameters and Biomarker Profile in a Cohort of Patients With Head and Neck Squamous Cell Carcinoma (HNSCC)

A new method for oral cancer biomarkers detection with a non-invasive cyto-salivary sampling and rapid-highly sensitive ELISA immunoassay: a pilot study in humans

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