Expression of Costimulatory Molecule CD28 on T Cells in Human Immunodeficiency Virus Type 1 Infection: Functional and Clinical Correlations

Brinchmann, Jan E.; Dobloug, Jan H.; Heger, Bernt; Haaheim, Lise L.; Sannes, Mette; Egeland, Torstein

doi:10.1093/infdis/169.4.730

Cited by 153 publications

(118 citation statements)

References 27 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…In agreement with previous studies [18][19][20][21][22], the proportion of CD8 CD28 asymptomatic individuals and patients with AIDS (mean in HIV-1-infected asymptomatic individuals 61%, in patients 80%, in healthy controls 24%) (Fig. 5a).…”

Section: Expansion Of Cd8 /Cd28 ÿ and Decrease Of Cd8 Cd28 T Cellssupporting

confidence: 92%

“…The resulting progressive decline in the proportion of CD8 CD28 cells during HIV-1 infection has been confirmed in other studies [19][20][21][22]. Also the proportion of CD4 /CD28 drops but the decline is much less pronounced than the decrease of CD8 / CD28 proportion [19][20][21][22]. We have reported as well that in the Amsterdam cohort of HIV-1-infected men low T cell reactivity correlated with progression to AIDS [12,23].…”

Section: Introductionsupporting

confidence: 84%

“…Decreased expression of CD28 on CD8 T cells, concomitantly resulting in increased percentages of CD28 has been repeatedly described in HIV-1-infected individuals [18][19][20][21][22]. We show that the expansion of CD8 CD28 ÿ T cells existed already in HIV -homosexual control individuals and remained at comparable levels in HIV-1-infected asymptomatic individuals and patients with AIDS.…”

Section: Discussionsupporting

confidence: 65%

“…Gruters et al [18] showed that in HIV-1-infected individuals from seroconversion to appearance of clinical symptoms an increase mainly in the number of CD8 CD28 ÿ T cells accounted for the increase of CD8 T cells and paralleled the appearance of cells expressing the CD38 marker. The resulting progressive decline in the proportion of CD8 CD28 cells during HIV-1 infection has been confirmed in other studies [19][20][21][22]. Also the proportion of CD4 /CD28 drops but the decline is much less pronounced than the decrease of CD8 / CD28 proportion [19][20][21][22].…”

Section: Introductionsupporting

confidence: 62%

“…It may be important to investigate homosexual men who are not at high risk of HIV-1 infection. Several investigators have demonstrated that CD3 CD28 ÿ T cells accumulate in HIV-1 infection but are unresponsive to CD3 MoAb, mitogens, CD28 MoAb and staphylococcal superantigens [20][21][22]31]. It has been suggested that CD8 CD28 ÿ T cells are generated as a result of an immunological event in the periphery [31].…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Low T cell reactivity to combined CD3 plus CD28 stimulation is predictive for progression to AIDS: correlation with decreased CD28 expression

Roos

Miedema

Meinesz

et al. 1996

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYIn 219 HIV-1-infected men of the Amsterdam cohort we measured CD4 T cell numbers and in vitro T cell responses to CD3 MoAbs with or without CD28 costimulation and phytohaemagglutinin (PHA). The value of these markers was estimated for disease progression within 4 years. CD28 expression on T cells has been related to T cell responses. CD28 costimulation considerably enhanced T cell reactivity ( 8-10-fold) with lower coefficients of variation compared with reactivity to CD3 MoAb alone (median 5 versus 20). T cell reactivity to CD3 plus CD28 MoAb was decreased during HIV-1 infection and was besides CD4 T cell numbers the only independent predictor for progression to AIDS. Compared with the group with high CD4 T cell numbers the relative risk (RR) for the group with intermediate levels was 2 . 28, with low levels 5 . 20. In the groups with intermediate and low CD3 plus CD28 responses the RR was 2 . 04 and 4 . 16, respectively. The combined RR for both was 4 . 65 and 21 . 63. The independence of this marker was confirmed when the group with low CD4 T cell numbers was subdivided into groups with high, intermediate and low T cell responses. The expansion of CD8 CD28 ÿ T cells was already apparent in HIV ÿ homosexual men, but CD8 CD28 T cells specifically decreased in patients with AIDS. CD28 expression on T cells correlated moderately with T cell responses to CD3 plus CD28 MoAb. T cell reactivity to CD3 MoAb in the presence of CD28 MoAb is a stronger prognostic marker than T cell reactivity to CD3 MoAb alone.

show abstract

Section: Expansion Of Cd8 /Cd28 ÿ and Decrease Of Cd8 Cd28 T Cellssupporting

confidence: 92%

Section: Introductionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 65%

Section: Introductionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Low T cell reactivity to combined CD3 plus CD28 stimulation is predictive for progression to AIDS: correlation with decreased CD28 expression

Roos

Miedema

Meinesz

et al. 1996

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

Measurement of CD69 induction in the assessment of immune function in asymptomatic HIV-infected individuals

et al. 1997

View full text Add to dashboard Cite

Peripheral blood mononuclear cells from many asymptomatic HIV‐infected patients exhibit defects in cytokine production and impaired proliferative responses in vitro but the mechanisms underlying this subclinical immune deficiency are controversial. To determine whether abnormalities in the earliest events following receptor engagement may help to explain the in vitro immune dysfunction, we measured the inducibility of the early activation marker CD69 in T cells from asymptomatic HIV‐infected individuals in response to stimulation with anti‐CD2 or anti‐CD3 mAb. In a whole blood assay, we found that induction of CD69 was markedly impaired in CD4+ T cells from later‐stage HIV‐infected patients (CD4 counts 200–400/mm3) compared to uninfected controls. Among early stage patients (CD4 > 400/mm3), a subset (29%) had impaired CD69 induction. CD69 responses were equally depressed after stimulation through the CD3 or CD2 receptor pathways. Survey of a panel of immunophenotypic markers and propensity for apoptosis demonstrated a significant association between depressed induction of CD69 and decreased percentages of CD4+CD26+ and CD4+CD95+ cells but no association with the level of apoptosis. These data indicate that defects in T lymphocyte activation through CD3 and CD2 can be measured within hours of receptor stimulation in asymptomatic HIV‐infected individuals and might be useful to monitor as an indicator of immune function in these patients. Cytometry 30:1–9, 1997. © 1997 Wiley‐Liss, Inc.

show abstract

Dysregulation of the interleukin-2 receptor ?- and ?-chain expression in CD4 and CD8 T cells in HIV infection

Johnson

Parkin

1997

Cytometry

View full text Add to dashboard Cite

Peripheral blood mononuclear cells were prepared from human immunodeficiency virus‐positive (HIV+) individuals (n = 46) to investigate the expression of both chains of the interleukin‐2 receptor. Both qualitative and quantitative changes in expression were observed. Total lymphocyte expression of the IL‐2α chain (CD25) was decreased compared with HIV‐negative (HIV−) controls. This was due to the decrease in the CD4+ population, which favored expression of this receptor, rather than a decrease in expression, per se. CD8+ lymphocytes expressed the β chain (CD122) in the absence of the α chain. However, a significant increase in the number of peripheral blood CD8+ lymphocytes expressing mainly the β subunit was observed in HIV+ patients (P = 0.02). This was observed to a similar extent in asymptomatic and symptomatic patients and characterized a subpopulation of T lymphocytes expressing high levels of CD8. Lymphocytes from patients with advanced HIV disease failed to up‐regulate both α and β chains in response to mitogenic concentrations of phytohaemagglutanin. However, those cells that were able to up‐regulate both of the IL‐2 receptors were capable of effective signal transduction through the receptor, increasing the proliferative response to stimulation. Cytometry 30:289–295, 1997. © 1998 Wiley‐Liss, Inc.

show abstract

Expression of Costimulatory Molecule CD28 on T Cells in Human Immunodeficiency Virus Type 1 Infection: Functional and Clinical Correlations

Cited by 153 publications

References 27 publications

Low T cell reactivity to combined CD3 plus CD28 stimulation is predictive for progression to AIDS: correlation with decreased CD28 expression

Low T cell reactivity to combined CD3 plus CD28 stimulation is predictive for progression to AIDS: correlation with decreased CD28 expression

Measurement of CD69 induction in the assessment of immune function in asymptomatic HIV-infected individuals

Dysregulation of the interleukin-2 receptor ?- and ?-chain expression in CD4 and CD8 T cells in HIV infection

Contact Info

Product

Resources

About