Expression of Collagen‐Binding Protein and Types 5 and 8 Capsular Polysaccharide in Clinical Isolates of<i>Staphylococcus aureus</i>

Ryding, Ulf; Flock, Jan‐Ingmar; Flock, Margareta; Söderquist, Bo; Christensson, Bértil

doi:10.1086/516520

Cited by 44 publications

(21 citation statements)

References 15 publications

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“…The fact that the three strains that lacked any form of sasG also lacked sarT and sarU is consistent with the results reported by Roche et al (53), who demonstrated that sasG is also included within RD5. With respect to the association between cna and serotype, our results are consistent with the results of Ryding et al (56), who found that cna was present almost exclusively in serotype 8 strains. However, it is unlikely that either of these is directly associated with biofilm formation based on previous studies from our laboratory demonstrating that at least some cnanegative, serotype 5 strains form a biofilm comparable to that of the UAMS isolates (5).…”

Section: Discussionsupporting

confidence: 92%

Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates

et al. 2005

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Much of the research aimed at defining the pathogenesis of Staphylococcus aureus has been done with a limited number of strains, most notably the 8325-4 derivative RN6390. Several lines of evidence indicate that this strain is unique by comparison to clinical isolates of S. aureus. Based on this, we have focused our efforts on two clinical isolates (UAMS-1 and UAMS-601), both of which are hypervirulent in our animal models of musculoskeletal infection. In this study, we used comparative genomic hybridization to assess the genome content of these two isolates relative to RN6390 and each of seven sequenced S. aureus isolates. Our comparisons were done by using an amplicon-based microarray from the Pathogen Functional Genomics Resource Center and an Affymetrix GeneChip that collectively represent the genomes of all seven sequenced strains. Our results confirmed that UAMS-1 and UAMS-601 share specific attributes that distinguish them from RN6390. Potentially important differences included the presence of cna and the absence of isaB, sarT, sarU, and sasG in the UAMS isolates. Among the sequenced strains, the UAMS isolates were most closely related to the dominant European clone EMRSA-16. In contrast, RN6390, NCTC 8325, and COL formed a distinct cluster that, by comparison to the other four sequenced strains (Mu50, N315, MW2, and SANGER-476), was the most distantly related to the UAMS isolates and EMRSA-16.Staphylococcus aureus is an opportunistic pathogen capable of causing serious human infections. An increasing number of these infections are recalcitrant to antimicrobial therapy. Indeed, the National Nosocomial Infections Surveillance System has seen a rise in oxacillin-resistant S. aureus (ORSA) among hospital isolates from approximately 4% in the 1980s to Ͼ50% in the late 1990s (47). Of recent concern is the increasing prevalence of ORSA among patients suffering from community-acquired infections (19). Research comparing these community-acquired ORSA isolates with hospital-acquired ORSA isolates found that the increase in community-acquired ORSA infections can be attributed to the acquisition of a novel SCCmec element (19). Furthermore, both vancomycin-intermediate and vancomycin-resistant S. aureus were recently described (66), confirming that S. aureus has the potential to resist even those antimicrobials often reserved as a "last resort." This emphasizes the evolutionary adaptability of S. aureus and highlights the importance of finding novel therapeutic approaches in the face of such a readily adaptable pathogen (50).Much of the research aimed at identifying novel therapeutic targets for the treatment of S. aureus infections has been directed at identification of specific virulence factors and regulatory circuits that are relevant to the disease process. Almost invariably, these studies used the prototypic lab strain NCTC 8325 or its derivatives. The single most widely studied strain is RN6390, which is an 8325-4 strain generated by curing three prophage from NCTC 8325 (1, 12, 45). However, RN6390 has an 11-b...

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Section: Discussionsupporting

confidence: 92%

Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates

et al. 2005

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“…acm was carried by all of the strains tested (n ϭ 32), but only 34.4% of the isolates expressed the ability to bind collagen, as a result of deletions, mutations, or the presence of insertion sequences in the gene or its promoter region (20). In contrast, only approximately 50% of S. aureus strains carry cna, although this usually correlates with the ability of the isolate to bind collagen (35).…”

Section: Discussionmentioning

confidence: 97%

The Arcanobacterium pyogenes Collagen-Binding Protein, CbpA, Promotes Adhesion to Host Cells

et al. 2003

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Arcanobacterium pyogenes is an opportunistic pathogen associated with suppurative diseases in economically important food animals such as cattle, pigs, and turkeys. A. pyogenes adheres to host epithelial cells, and adhesion is promoted by the action of neuraminidase, which is expressed by this organism. However, a neuraminidase-deficient mutant of A. pyogenes only had a reduced ability to adhere to host epithelial cells, indicating that other factors are involved in adhesion. Far Western blotting revealed the presence of an approximately 120-kDa A. pyogenes cell wall protein that binds collagen type I. The 3.5-kb gene that encodes the 124.7-kDa CbpA protein was cloned, and sequence analysis indicated that CbpA contains a typical MSCRAMM protein domain structure. Recombinant, six-His-tagged CbpA (HIS-CbpA) was capable of binding collagen types I, II, and IV but not fibronectin. In addition, CbpA was involved in the ability of A. pyogenes to adhere to HeLa and 3T6 cells, as a cbpA knockout strain had 38.2 and 57.0% of wild-type adhesion, respectively. This defect could be complemented by providing cbpA on a multicopy plasmid. Furthermore, HIS-CbpA blocked A. pyogenes adhesion to HeLa or 3T6 cells in a dose-dependent manner. cbpA was only present in 48% of the A. pyogenes strains tested (n ‫؍‬ 75), and introduction of plasmid-encoded cbpA into a naturally cbpA-deficient strain increased the ability of this strain to bind to HeLa and 3T6 cells 2.9-and 5.7-fold, respectively. These data indicate that CbpA, a collagen-binding protein of A. pyogenes, plays a role in the adhesion of this organism to host cells.

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“…Unlike clinical isolates of Pseudomonas aeruginosa from patients with CF (reviewed in reference 8), S. aureus produces very little PIA/PNSG in vitro on culture plates but produces predominantly polysaccharide microcapsules of type 5 or 8 (1,14,27,29). Due to this in vitro phenotype, most investigators have thought that S. aureus would also express CP5 or CP8 during infections in animals or humans.…”

Section: Discussionmentioning

confidence: 99%

Anaerobic Conditions Induce Expression of Polysaccharide Intercellular Adhesin in Staphylococcus aureus and Staphylococcus epidermidis

et al. 2001

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Products of the intercellular adhesion (ica) operon in Staphylococcus aureusand Staphylococcus epidermidis synthesize a linear ␤-1,6-linked glucosaminylglycan. This extracellular polysaccharide mediates bacterial cell-cell adhesion and is required for biofilm formation, which is thought to increase the virulence of both pathogens in association with prosthetic biomedical implants. The environmental signal(s) that triggers ica gene product and polysaccharide expression is unknown. Here we demonstrate that anaerobic in vitro growth conditions lead to increased polysaccharide expression in both S. aureus and S. epidermidis, although the regulation is less stringent in S. epidermidis. Anaerobiosis also dramatically stimulates ica-specific mRNA expression in ica-and polysaccharide-positive strains of both S. aureus and S. epidermidis. These data suggest a mechanism whereby ica gene expression and polysaccharide production may act as a virulence factor in an anaerobic environment in vivo.

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Expression of Collagen‐Binding Protein and Types 5 and 8 Capsular Polysaccharide in Clinical Isolates ofStaphylococcus aureus

Cited by 44 publications

References 15 publications

Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates

Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates

The Arcanobacterium pyogenes Collagen-Binding Protein, CbpA, Promotes Adhesion to Host Cells

Anaerobic Conditions Induce Expression of Polysaccharide Intercellular Adhesin in Staphylococcus aureus and Staphylococcus epidermidis

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