Expression of Col1a1, Col1a2 and procollagen I in germ cells of immature and adult mouse testis

He, Zuping; Feng, Lixin; Xiao-dong, Zhang; Geng, Yixun; Parodi, Daniela A.; Suárez‐Quian, Carlos A.; Dym, Martin

doi:10.1530/rep.1.00694

Cited by 58 publications

(53 citation statements)

References 30 publications

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“…RNA in situ hybridization was also employed to confirm the microarray data showing that Sly transcript was predominantly expressed in spermatids (Toure et al 2005). In our laboratory, we corroborated the microarray data showing col1a1 and col1a2 mRNA expression levels in the seminiferous tubules of immature and adult mice using semi-quantitative RT-PCR (He et al 2005). Adachi and colleagues revealed that 11 genes were upregulated and three genes were downregulated by diethylstilbestrol in 28-day-old mice using cDNA microarray analysis, however, they only confirmed eight out of 11 upregulated genes by real-time RT-PCR (Adachi et al 2002).…”

Section: Reproducibility Is Essential For the Application Of Microarrsupporting

confidence: 74%

Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertility

He¹,

Chan²,

Dym³

2006

Reproduction

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Male infertility is now a major reproductive health problem because of an increasing number of environmental pollutants and chemicals, which eventually result in gene mutations. Genetic alterations caused by environmental factors account for a significant percentage of male infertility. Microarray technology is a powerful tool capable of measuring simultaneously the expression of thousands of genes expressed in a single sample. Eventually, advances in genetic technology will allow for the diagnosis of patients with male infertility due to congenital reasons or environmental factors. Since its introduction in 1994, microarray technology has made significant advances in the identification and characterization of novel or known genes possibly correlated with male infertility in mice, as well as in humans. This provides a rational basis for the application of microarray to establishing molecular signatures for the diagnosis and gene therapy targets of male infertility. In this review, the differential gene expression patterns characterized by microarray in germ and somatic cells at different steps of development or in response to stimuli, as well as a number of novel or known genes identified to be associated with male infertility in mice and humans, are addressed. Moreover, issues pertaining to measurement reproducibility are highlighted for the application of microarray data to male infertility.

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Section: Reproducibility Is Essential For the Application Of Microarrsupporting

confidence: 74%

Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertility

He¹,

Chan²,

Dym³

2006

Reproduction

Self Cite

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“…Of these, the first three were reported in testis at transcript level (The MGC Project Team 2004, He et al 2005 while the fourth one was reported at protein level (Emkey et al 1991).…”

Section: Discussionmentioning

confidence: 99%

Identification of novel immunodominant epididymal sperm proteins using combinatorial approach

Khan

Suryawanshi²,

Ranpura³

et al. 2009

Reproduction

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Functionally immature spermatozoa leave the testis mature during epididymal transit. This process of maturation involves either addition of new proteins or modification of existing proteins onto the sperm domains that are responsible for domain-specific functions. Epididymal proteins are preferred targets for immunocontraception. In an attempt to identify epididymis-specific sperm proteins, we used a novel combinatorial approach comprising subtractive immunization (SI) followed by proteomics. Following SI, sera of mice were used for immunoproteomics, which led to the identification of 30 proteins, of which four proteins namely sperm head protein 1, sperm flagella protein 2 (SFP2), SFP3, and SFP4 are being reported for the first time on sperm. Another group of four proteins namely collagen a-2 (I) chain precursor, homeodomain-interacting protein kinase 1, GTP-binding protein Rab1, and ubiquinol cytochrome c reductase core protein II although reported earlier in testis are being reported for the first time in epididymal sperm. Furthermore, seven out of these eight novel proteins could be validated using peptide ELISA. These data are a useful repository, which could be exploited to develop targets for post-testicular immunocontraception or biomarkers for infertility diagnosis and management.

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“…The distribution of procollagen I within the seminiferous tubules of immature and adult mice correlates with the process of germ cell attachment and detachment from the basement membrane. The unique distribution pattern of procollagen I in adult mouse testes implies a possible role for COL1A1, COL1A2, and procollagen I in regulating the adhesion of spermatogonia and preleptotene spermatocytes to the basement membrane and the detachment and migration of spermatocytes and spermatids towards the lumen during spermatogenesis (43).…”

Section: Discussionmentioning

confidence: 99%

CYP2E1 Testis Expression and Alcoholmediated Changes of Rat Spermatogenesis Indices and Type I Collagen

Shayakhmetova

Bondarenko

Коваленко

et al. 2013

Archives of Industrial Hygiene and Toxicology

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This study is a complex investigation of alcohol-mediated changes in CYP2E1 mRNA and protein expression in the testes, as well as spermatogenesis indices and type I collagen amino acid contents, in male rats. Wistar albino male rats were divided into two groups: I -control (intact animals), II -experimental (chronic alcoholism, exposure to a 15 % ethanol aqueous solution during 150 days). The destructive changes in the spermatogenic epithelium were accompanied by a decrease in sperm number and motility time. CYP2E1 mRNA and protein expression were elevated in the testes 3 and 1.4 times, respectively. Also, signifi cantly lower contents of lysine, glutamic acid, serine, proline, alanine, valine, and phenylalanine residues accompanied by an increase of hydroxyproline, glycine, and threonine residue contents were detected in the skin type I collagen of the experimental group. Chronic ethanol consumption caused testicular failure along with an overexpression of CYP2E1 mRNA and protein in the testes as well as quantitative changes in type I collagen amino acid contents. The profound alcohol-mediated changes in collagen type I amino acid contents may have affected the spermatogenic epithelium state. The modulation of testicular cytochrome P450 2E1 mRNA and protein expression could change the functioning of this isozyme in target organs and take part in the mechanism of ethanol gonadotoxicity.

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Expression of Col1a1, Col1a2 and procollagen I in germ cells of immature and adult mouse testis

Cited by 58 publications

References 30 publications

Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertility

Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertility

Identification of novel immunodominant epididymal sperm proteins using combinatorial approach

CYP2E1 Testis Expression and Alcoholmediated Changes of Rat Spermatogenesis Indices and Type I Collagen

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