Expression of claudin-11 in canine prepubertal testes, and in canine adult testes showing normal spermatogenesis, impaired spermatogenesis, or testicular neoplasia

Pörtner, Carolin; Rode, Kristina; Hollenbach, Julia; Thiemeyer, Heike; Beineke, Andreas; Günzel‐Apel, A.‐R.; Brehm, Ralph

doi:10.1016/j.theriogenology.2020.03.001

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…Compared with the preweaning piglet testes, in the cryptorchid testes, the number of Sertoli and germ cells increased, the gonocytes differentiated into spermatogonia, and the testis cords developed into seminiferous tubules. These results are consistent with previous findings on cryptorchidism in horses [ 30 ] and dogs [ 31 ], suggesting that cryptorchidism did not affect the formation of seminiferous tubules and the differentiation of gonocytes into spermatogonia.…”

Section: Discussionsupporting

confidence: 93%

“…Only Sertoli cells with oval nuclei and gonocytes were detected in the testicular tissue of preweaning piglets, and the lumen was not identified. Similar morphological alterations have been reported in spontaneous and surgically induced unilateral cryptorchidism rats [29], boars [15], horses [30], dogs [31], and rabbits [8], in which the spermatogenic epithelium of the cryptorchid testes comprised only Sertoli cells and several spermatogonia and did not contain post-meiotic germ cells.…”

Section: Discussionsupporting

confidence: 79%

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Effect of Cryptorchidism on the Histomorphometry, Proliferation, Apoptosis, and Autophagy in Boar Testes

Fan

Liu

Yue

et al. 2021

Animals

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Spontaneous unilateral cryptorchid boars have one testis in the abdomen or inguinal canal, causing its temperature to be at or near the body temperature, which impairs spermatogenesis, although the histomorphometry and molecular mechanisms underlying this process remain unclear. The aim of the present study was to determine the histomorphometry, proliferation, apoptosis, and autophagy alterations in spermatogonia and Sertoli cells in unilateral cryptorchid, scrotal (contrascrotal), and preweaning piglet (preweaning) testes. Histomorphometrical analysis of cryptorchid testes showed that the seminiferous tubules contained only Sertoli cells and a few spermatogonia, but did not contain post-meiotic germ cells. The number of spermatogonia markedly decreased, and the number of Sertoli cells did not change remarkably in cryptorchid testes. TUNEL assay results showed that apoptosis signals were predominantly observed in spermatogonia. In cryptorchid and contrascrotal testes, proliferating cell nuclear antigen (PCNA) and LC3 were located in spermatogonia. The number of PCNA-positive, TUNEL-positive, and LC3-positive germ cells was low, and the protein and mRNA levels of PCNA and LC3 were significantly decreased in cryptorchid testes. Taken together, the number of Sertoli cells did not change remarkably, whereas the number of germ cells decreased in the cryptorchid testes, compared with that in the contrascrotal testes. Insufficient proliferation, excessive apoptosis, and autophagy were involved in the regulation of the decrease in spermatogonia in cryptorchid boar testes.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 79%

Effect of Cryptorchidism on the Histomorphometry, Proliferation, Apoptosis, and Autophagy in Boar Testes

Fan

Liu

Yue

et al. 2021

Animals

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“…Previous studies have demonstrated that tight junctions in BTB and BEB are composed of various claudin proteins. Up‐to‐date, claudins 1, 3, 4, 5, 7, 8, and 11 have been found in the testis of some mammals, such as mice (Chihara et al, 2010; Gye, 2003; Mazaud‐Guittot et al, 2010; Meng et al, 2005; Morita, Sasaki, Fujimoto, et al, 1999; Morrow et al, 2009, 2010; Stammler et al, 2016), rat (Kamimura et al, 2002;Manku et al, 2016; Stammler et al, 2016), pig (Park et al, 2011), alpaca (Guo et al, 2013), bovine (McMillan et al, 2014), horse (Lee et al, 2016; Rode et al, 2015), human (Manku et al, 2016; Stammler et al, 2016), and dog (Pörtner et al, 2020). Studies have revealed that some claudins exhibit a spermatogenic stage cycle‐specific localization pattern in some species (McMillan et al, 2014; Morrow et al, 2010) but are present in all phases of the cycle in others (Mazaud‐Guittot et al, 2010; Tarulli et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Of the claudin proteins, claudins 3 and 11 have received the most attention (Morita, Sasaki, Fujimoto, et al, 1999; Mazaud‐Guittot et al, 2010; Morrow et al, 2010; Park et al, 2011; Guo et al, 2013; Rode et al, 2015; Stammler et al, 2016; Pörtner et al, 2020); however, the expression and localization of claudin‐1 and ‐5 in BTB and BEB tight junctions have been studied less. Claudin‐1, the first identified claudin, is crucial to mammal epidermal barrier formation by forming TJ strands (Furuse et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

The abundance and localization of claudin‐1 and ‐5 in the adult tomcats (Felis catus) testis, tubules rectus, rete testis, efferent ductules, and epididymis

Lïman

2023

The Anatomical Record

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Tight junctions (TJ) are the anatomical component of blood-testis (BTB) and blood-epididymis (BEB) barriers and contain many proteins, including claudins. The presence of claudins in domestic cat testis and epididymis has not been previously described. This study aimed to determine whether claudin-1 and claudin-5 participate in the structure of BTB and BEB and whether their amounts differ between the testis and epididymal segments of adult cats, using Western blotting (WB) and immunohistochemistry. WB results demonstrated that claudin-1 was significantly lower in the testis than in all epididymal segments and higher in the corpus epididymis than in the cauda, while claudin-5 in the testis was significantly lower than in the caput and corpus. Claudin-1 was absent at the Sertoli-Sertoli junctions, while claudin-5 was detected at the level of the BTB during stages I and VIII. Both claudins were observed in the pachytene spermatocytes and the developing acrosome of the round and elongating spermatids. Claudin-5 was also detected in the cytoplasm of some spermatogonia, Sertoli cells, and late spermatid acrosome. In the epididymal segments, both claudins were localized to the area of the tight junctions and along the entire length of the lateral plasma membranes of adjacent principal cells and between principal and basal cells. These results may indicate that in the domestic cat, claudin-1 and -5 participate as both tight junction proteins and adhesion molecules in the BEB's structure, claudin 5 is a component of the BTB, and both proteins may be involved in postmeiotic germ cell development, especially acrosome development.

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Identification of a circRNA-miRNA-mRNA network to explore the effects of circRNAs on Holstein bull testis after sexual maturity

Sahlu,

Wang,

et al. 2023

Animal Reproduction Science

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Expression of claudin-11 in canine prepubertal testes, and in canine adult testes showing normal spermatogenesis, impaired spermatogenesis, or testicular neoplasia

Cited by 6 publications

References 47 publications

Effect of Cryptorchidism on the Histomorphometry, Proliferation, Apoptosis, and Autophagy in Boar Testes

Effect of Cryptorchidism on the Histomorphometry, Proliferation, Apoptosis, and Autophagy in Boar Testes

The abundance and localization of claudin‐1 and ‐5 in the adult tomcats (Felis catus) testis, tubules rectus, rete testis, efferent ductules, and epididymis

Identification of a circRNA-miRNA-mRNA network to explore the effects of circRNAs on Holstein bull testis after sexual maturity

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