Expression of class II β‐tubulin in non‐melanoma cutaneous tumors

Roh, Joo Young; Kee, Sun Ho; Choi, Jung Woo; Lee, Ji Hye; Lee, Ju Han; Lee, Eung Seok; Kim, Young Sik

doi:10.1111/j.1600-0560.2006.00583.x

Cited by 7 publications

(7 citation statements)

References 30 publications

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“…Tubulin is known to form microtubules and the centrosome and is an important protein in the differentiation of cells including keratinocytes (34). Both α and β-tubulin decreased in alopecic mice ( Table I), suggesting that cell proliferation of keratinocyte was decreased in alopecic mice.…”

Section: Discussionmentioning

confidence: 97%

Damage of hair follicle stem cells and alteration of keratin expression in external radiation-induced acute alopecia

Nanashima

Ito

Ishikawa

et al. 2012

International Journal of Molecular Medicine

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Abstract. Alopecia is known as a symptom of acute radiation, yet little is known concerning the mechanism of this phenomenon and the alteration of hair protein profiles. To examine this, 6-week-old male C57/BL6 mice were exposed to 6 Gy of X-ray irradiation, which caused acute alopecia. Their hair and skin were collected, and hair proteins were analyzed with liquid chromatography/electrospray-ionization mass spectrometry and immunohistochemistry. No change was observed in the composition of major hair keratins, such as Krt81, Krt83 and Krt86. However, cytokeratin Krt15 and CD34, which are known as hair follicle stem cell markers, were decreased in alopecic mice. Cytokeratin Krt5, which is known as a marker for basal and undifferentiated keratinocytes, was increased in the epidermis of alopecic mice. These findings suggest that radiation damages hair stem cells and the differentiation of keratinocytes in the epidermis. For the evaluation of radiation exposure, chromosomal aberration is considered to be the gold standard, yet our results suggest that Krt5 may be a novel biological marker for acute radiation symptoms.

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Section: Discussionmentioning

confidence: 97%

Damage of hair follicle stem cells and alteration of keratin expression in external radiation-induced acute alopecia

Nanashima

Ito

Ishikawa

et al. 2012

International Journal of Molecular Medicine

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“…However, ß II expression is not directly associated with neuroendocrine differentiation in the cutaneous tumors, because ß II is expressed in the keratinocytes of the granular layer, hair cortical and cuticular cells, inner root sheath and companion layer of the outer root sheath in the normal skin and up-regulated during squamous differentiation of keratinocytes (20). Roh et al also reported that ß II expression was increased in the areas of squamous or follicular differentiation in the cutaneous tumors (19). Thus, ß II expression in BCCs is likely not the predominant phenotype related to the neuroendocrine differentiation.…”

Section: Discussionmentioning

confidence: 96%

“…It is widely distributed in the developing neuronal axons and dendrites as well as neuroepithelial tumors and represents a marker for progenitor and neuronal stem cells (18). Roh et al reported that ß II was focally expressed in approximately half of BCCs (32/57 cases) (19). However, ß II expression is not directly associated with neuroendocrine differentiation in the cutaneous tumors, because ß II is expressed in the keratinocytes of the granular layer, hair cortical and cuticular cells, inner root sheath and companion layer of the outer root sheath in the normal skin and up-regulated during squamous differentiation of keratinocytes (20).…”

Section: Discussionmentioning

confidence: 99%

Aberrant expression of class III β-tubulin in basal cell carcinoma of the skin

Ishida

Kushima²,

Okabe³

2009

Oncol Rep

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Abstract. Tubulin is a major component of microtubules. Class III ß-tubulin (ß III) is a neuron-associated ß-tubulin isotype and expressed in the normal central and peripheral nervous systems. According to a previous study, ß III is not expressed in normal skin and squamous cell carcinoma. However, its expression has not been examined in basal cell carcinoma (BCC) of the skin. Expression of ß III was analyzed together with neural cell adhesion molecule (NCAM), chromogranin A, synaptophysin, epithelial membrane antigen (EMA) and cytokeratin (CK) 20 by immunohistochemical methods in 10 non-neoplastic skin tissues and 50 BCCs. In the normal skin, immunoreactivity to ß III was restricted to the nerve bundles in the dermis and subcutis, no positivity was shown in epithelial cells of the epidermis and skin appendages. ß III and NCAM were expressed in 50 and 68% of BCCs, respectively, predominantly periphery of tumor nests, although the distribution of both markers was not always identical. Chromogranin A, synaptophysin and CK 20 were not expressed in any of BCCs. EMA was focally expressed in only 8% of BCCs. ß III is a potential candidate for inclusion to the panel of immunohistochemical markers to distinguish small BCCs from non-neoplastic hair buds, because non-neoplastic hair follicles are not positive for ß III.

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“…Isotype expression differs amongst cancers. βIII has been detected as a potential marker in squamous, ovarian, and neuroendocrine cancer [20][21][22], while βII-tubulin has been proven to be useful in differentiating skin tumors and predicting the response to treatment in prostate cancer [23,24]. For colorectal cancer, tubulin expression has been correlated with disease stage [25,26], while analysis of colorectal cancer cell lines has revealed the predominant expression of βI, Fig.…”

Section: Discussionmentioning

confidence: 99%

Identification of β-tubulin as a common immunogen in gastrointestinal malignancy by mass spectrometry of colorectal cancer proteome: implications for early disease detection

et al. 2012

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Expression of class II β‐tubulin in non‐melanoma cutaneous tumors

Abstract: Class II tubulin expression is closely related to squamous or follicular differentiation and may be helpful in distinguishing most SCCs from KAs and BCC from TE. However, it does not reliably distinguish well-differentiated, crateriform SCC from KA.

Cited by 7 publications

References 30 publications

Damage of hair follicle stem cells and alteration of keratin expression in external radiation-induced acute alopecia

Damage of hair follicle stem cells and alteration of keratin expression in external radiation-induced acute alopecia

Aberrant expression of class III β-tubulin in basal cell carcinoma of the skin

Identification of β-tubulin as a common immunogen in gastrointestinal malignancy by mass spectrometry of colorectal cancer proteome: implications for early disease detection

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