Expression of CHOP in Squamous Tumor of the Uterine Cervix

Abstract

BackgroundHigh-risk human papillomavirus (HR-HPV) infection and abnormal p53 expression are closely involved in carcinogenesis of squamous cell carcinoma (SqCC) of uterine cervix. Recent studies have suggested that virus-induced endoplasmic reticulum (ER) stress modulates various cell survival and cell death signaling pathways. The C/EBP homologous protein (CHOP) is associated with ER stress-mediated apoptosis and is also involved in carcinogenesis of several human cancers. We hypothesized that CHOP is involved in the carcinogenesis of uterine cervical cancer in association with HR-HPV and/or p53.MethodsImmunohistochemistry was used to analyze CHOP and p53 protein expression of tissue sections from 191 patients with invasive cancer or preinvasive lesions of the uterine cervix (61 cases of SqCC, 66 cases of cervical intraepithelial neoplasia [CIN] III, and 64 cases of CIN I).ResultsCHOP was expressed in 59.4% of CIN I, 48.5% of CIN III, and 70.5% of SqCC cases. It was also significantly more frequent in invasive SqCC than in preinvasive lesions (p=0.042). Moreover, CHOP expression significantly correlated with HR-HPV infection and p53 expression (p=0.009 and p=0.038, respectively).ConclusionsOur results suggest that CHOP is involved in the carcinogenesis of the uterine cervix SqCC via association with HR-HPV and p53.

Endoplasmic reticulum stress pathway PERK‐eIF2α confers radioresistance in oropharyngeal carcinoma by activating NF‐κB

“…Recent studies indicated that HPV could cause misfolded and unfolded proteins in infected cells to induce ERS and participate in the regulation of the survival and death of tumor cells. (30) The regulation of ERS by HPV infection had tumor specificity. In addition, ERS was inhibited in HPV16 (+) genital epithelial keratinocytes.…”

Attenuation of steatohepatitis, fibrosis, and carcinogenesis in mice fed a methionine‐choline deficient diet by CCAAT/enhancer‐binding protein homologous protein deficiency

“…Thus, it is important to clarify whether CHOP is involved in fat‐related liver injury. Moreover, some reports indicated that CHOP is involved in the pathogeneses of malignancies, including sarcoma and squamous tumors of the uterine cervix . However, the involvement of CHOP in hepatic carcinogenesis is yet to be defined.…”

“…Moreover, some reports indicated that CHOP is involved in the pathogeneses of malignancies, including sarcoma and squamous tumors of the uterine cervix. [17][18][19] However, the involvement of CHOP in hepatic carcinogenesis is yet to be defined. Thus, the purpose of our study was to evaluate the involvement of CHOP in the development of fat-induced liver injury and consequent inflammation, fibrosis, and carcinogenesis, using mice lacking the CHOP gene.…”

MG289 in <i>Mycoplasma genitalium</i> Enhances Microbial Invasion and Bacterial Persistence in Benign Human Prostate Cells

“…Activation of UPR is also associated with infectioninduced carcinogenesis [52,53]. Increased Hspa5 and Chop expression is specifically associated with Helicobacter felis induced gastric dysplasia in C57BL/6 mice [52].…”

“…Increased Hspa5 and Chop expression is specifically associated with Helicobacter felis induced gastric dysplasia in C57BL/6 mice [52]. Enhanced CHOP expression is associated with human papillomavirus (HR-HPV) infection and abnormal p53 expression in squamous cell carcinoma of uterine cervix [53]. Therefore, the increased expression of CHOP in M. genitalium WT infected cells, may also be associated with malignant transformation of BPH-1 cells during chronic exposure to M. genitalium [8].…”