Expression of cementum-derived attachment protein in bovine tooth germ during cementogenesis

Saito, Masahiro; Iwase, Masahiro; Maslan, S; Nozaki, Naohito; Yamauchi, Masato; Handa, Keisuke; Takahashi, Osamu; Sato, Sadao; Kawase, Toshio; Teranaka, Toshio; Narayanan, A. Sampath

doi:10.1016/s8756-3282(01)00573-7

Cited by 54 publications

(53 citation statements)

References 35 publications

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“…Cementum attachment prodated in the future study. tein (CAP) and cementum protein (CP) were identified derived cells as well as the cells in the cementoid layer and PDL space (3)(4)(5)38,47,52). We confirmed periodon-REFERENCES tal tissue regeneration using anti-rhCEMP1 antibody,…”

Section: Generation Of Periodontitis In Beagle Dogsupporting

confidence: 55%

Efficacy of Periodontal Stem Cell Transplantation in the Treatment of Advanced Periodontitis

2011

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Periodontitis is the most common cause for tooth loss in adults and advanced types affect 10-15% of adults worldwide. The attempts to save tooth and regenerate the periodontal apparatus including cementum, periodontal ligament, and alveolar bone reach to the dental tissue-derived stem cell therapy. Although there have been several periodontitis models suggested, the apical involvement of tooth root is especially challenging to be regenerated and dental stem cell therapy for the state has never been investigated. Three kinds of dental tissue-derived adult stem cells (aDSCs) were obtained from the extracted immature molars of beagle dogs (n = 8), and ex vivo expanded periodontal ligament stem cells (PDLSCs), dental pulp stem cells (DPSCs), and periapical follicular stem cells (PAFSCs) were transplanted into the apical involvement defect. As for the lack of cementum-specific markers, anti-human cementum protein 1 (rhCEMP1) antibody was fabricated and the aDSCs and the regenerated tissues were immunostained with anti-CEMP1 antibody. Autologous PDLSCs showed the best regenerating capacity of periodontal ligament, alveolar bone, and cementum as well as peripheral nerve and blood vessel, which were evaluated by conventional and immune histology, 3D micro-CT, and clinical index. The rhCEMP1 was expressed strongest in PDLSCs and in the regenerated periodontal ligament space. We suggest here the PDLSCs as the most favorable candidate for the clinical application among the three dental stem cells and can be used for treatment of advanced periodontitis where tooth removal was indicated in the clinical cases.

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Section: Generation Of Periodontitis In Beagle Dogsupporting

confidence: 55%

Efficacy of Periodontal Stem Cell Transplantation in the Treatment of Advanced Periodontitis

2011

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“…This antibody was raised against partially purified bovine cementum matrix containing cementum-derived attachment protein and specifically recognizes cementum matrix and cementoblasts but not tissues such as bone matrix. (11) Although these results support the presence of cementoblast progenitors in the dental follicle, details of the progenitor biology and their differentiation potential remain largely unknown.…”

Section: Introductionmentioning

confidence: 84%

Immortalization of Cementoblast Progenitor Cells With Bmi-1 and TERT

Saito

Handa

Kiyono³

et al. 2005

Journal of Bone and Mineral Research

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A cementoblast progenitor cell line designated BCPb8 was successfully isolated from dental follicle cells immortalized with Bmi-1 and hTERT. BCPb8 showed the potential to differentiate into cementoblasts on implantation into immunodeficient mice. BCPb8 was confirmed to be the first established cementoblast progenitor cell line and will provide a useful model for investigating cementogenesis. Introduction:The dental follicle is the mesenchymal tissue surrounding the developing tooth germ. During tooth root development, progenitor cells present in the dental follicle are believed to play a central role in the formation of periodontal components (cementum, periodontal ligament, and alveolar bone). However, little more is known about the biology of these progenitors. Previously, we observed that cultured bovine dental follicle cells (BDFCs) contained putative cementoblast progenitors. To further analyze the biology of these cells, we attempted to isolate cementoblast progenitors from immortalized BDFC through expression of the polycomb group protein, Bmi-1, and human telomerase reverse transcriptase (hTERT). Materials and Methods: BDFCs were transduced with replication-deficient retroviruses carrying human Bmi-1(LXSN-Bmi-1), and hTERT (LXSH-hTERT) for immortalization. Single cell clones were established from immortalized BDFC, and differentiation into cementoblasts was assessed by implantation into immunodeficient mice. Results and Conclusion: BDFCs expressing Bmi-1 and hTERT showed an extended life span-90 population doublings more than normal BDFCs-and still contained cells with the potential to differentiate into cementoblasts on implantation into immunodeficient mice. From these cells, we established a clonal cell line, designated BCPb8, which formed cementum-like tissue that was reactive to the anti-cementum-specific monoclonal antibody 3G9 and expressed mRNA for bone sialoprotein, osteocalcin, osteopontin, and type I collagen on implantation. Thus, by using Bmi-1 and hTERT, we succeeded in immortalizing cementoblast progenitor cells from BDFC without affecting differentiation potential. The BCPb8 cell line is the first immortalized clonal cell line of cementoblast progenitors and could be a useful tool not only to study cementogenesis but also to develop regeneration therapy for patients with periodontitis.

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“…9 Additionally, cementoblasts specifically express cementum-derived attachment protein (CAP) [10][11][12] and cementum-derived growth factor (CGF), 13,14 the unique biological markers for cementoblasts. 15,16 BSP, one of the major proteins in the cementum, is a highly sulphated, phosphorylated, and glycosylated protein that plays an essential role in cementogenesis. 15 A relationship has been noted between the speed of cementogenesis and the amount of BSP present in cementum.…”

Section: Introductionmentioning

confidence: 99%

Mechanical Tensile Stress Effects on the Expression of Bone Sialoprotein in Bovine Cementoblasts

Yu¹,

Ren

Sandham

et al. 2009

The Angle Orthodontist

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Objective: To develop a new cementoblast culture method and to detect bone sialoprotein (BSP) expression in response to high and low mechanical tensile stress in cementoblast in vitro. Materials and Methods: Cementoblasts were collected from the roots of newborn bovine teeth and were identified with cementum-derived attachment protein (CAP) antibody 3G9. Cell proliferation was evaluated by MTT [3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay, and mineralization was confirmed by von Kossa staining. Mechanical tensile stress was applied in vitro to the cementoblast with the use of a uniaxial four-point bending system with 2000 or 4000 microstrains, at a frequency of 0.5 Hz for 3, 6, 12, 24, or 36 hours. BSP mRNA level was quantified by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Results: A large amount of cementoblast was observed to be expressing CAP. Cementoblasts had a proliferation tendency similar to that of osteoblasts but different from that of periodontal ligament (PDL) cells. Cementoblasts had the ability to become mineralized between osteoblasts and PDL cells. The mechanical tensile stress significantly up-regulated BSP mRNA expression, which reached a peak at 24 hours in both 2000 and 4000 microstrain groups (P Ͻ .01) and was tenfold and sixfold higher than that of controls, respectively. BSP expression dropped toward baseline levels at 36 hours in both groups. Conclusions: Mechanical tensile stress up-regulated the expression of BSP. Low mechanical tensile stress induced earlier and more intensive up-regulation of BSP mRNA; this might represent the optimal stimuli for cementoblast activity. (Angle Orthod. 2009;79:346-352.)

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Expression of cementum-derived attachment protein in bovine tooth germ during cementogenesis

Cited by 54 publications

References 35 publications

Efficacy of Periodontal Stem Cell Transplantation in the Treatment of Advanced Periodontitis

Efficacy of Periodontal Stem Cell Transplantation in the Treatment of Advanced Periodontitis

Immortalization of Cementoblast Progenitor Cells With Bmi-1 and TERT

Mechanical Tensile Stress Effects on the Expression of Bone Sialoprotein in Bovine Cementoblasts

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