Expression of CD9 and CD81 in bovine germinal vesicle oocytes after vitrification followed by in vitro maturation

Ma, YingLiang; Pan, Bo; Yang, Huai; Qazi, Izhar Hyder; Wu, Zhenzheng; Zeng, Changjun; Zhang, Ming; Meng, Qingyong; Zhou, Guangbin

doi:10.1016/j.cryobiol.2018.02.011

Cited by 11 publications

(15 citation statements)

References 12 publications

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“…However, the survival rate of oocytes and their subsequent developmental competence are decreased significantly after vitrification when compared with these characteristics of fresh oocytes [11,12,13]. The decreased developmental potential due to oocyte cryopreservation may inevitably result from the alteration of intracellular levels of reactive oxygen species (ROS) [14] or glutathione (GSH) [15], and/or gene expression [16,17,18].…”

Section: Introductionmentioning

confidence: 99%

Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression

Pan

Yang

et al. 2018

IJMS

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This study aimed to investigate the effect of melatonin on the cell cycle of parthenogenetic embryos derived from vitrified mouse metaphase II (MII) oocytes. Fresh oocytes were randomly allocated into three groups: untreated (control), or vitrified by the open-pulled straw method without (Vitrification group) or with melatonin (MT) supplementation (Vitrification + MT group). After warming, oocytes were parthenogenetically activated and cultured in vitro, then the percentage of embryos in the G1/S phase, the levels of reactive oxygen species (ROS) and glutathione (GSH), and the mRNA expression of cell cycle-related genes (P53, P21 and E2F1) in zygotes and their subsequent developmental potential in vitro were evaluated. The results showed that the vitrification/warming procedures significantly decreased the frequency of the S phase, markedly increased ROS and GSH levels and the expression of P53 and P21 genes, and decreased E2F1 expression in zygotes at the G1 stage and their subsequent development into 2-cell and blastocyst stage embryos. However, when 10−9 mol/L MT was administered for the whole duration of the experiment, the frequency of the S phase in zygotes was significantly increased, while the other indicators were also significantly improved and almost recovered to the normal levels shown in the control. Thus, MT might promote G1-to-S progression via regulation of ROS, GSH and cell cycle-related genes, potentially increasing the parthenogenetic development ability of vitrified–warmed mouse oocytes.

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Section: Introductionmentioning

confidence: 99%

Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression

Pan

Yang

et al. 2018

IJMS

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“…2 Epigenetic effects of oocyte vitrification in humans and animals (a global overview). Numbers in brackets mean the number of studies reporting differences out of the total number of studies in the literature consistently reported (Table 4) [113,114,[129][130][131]133]. However, Chen et al recently reported an upregulation of two imprinted genes that are known to play a crucial role in development (Gtl2 and Peg10 ) [130].…”

Section: Impact Of Oocyte Vitrification On Gene Expressionmentioning

confidence: 99%

“…With GO and KEGG analyses, several pathways were identified: transcription regulation, cell differentiation and mitosis, regulation of actin cytoskeleton, and apoptosis. Ma et al [ 131 ] Bovine MII after IVM from fresh GV, MII after IVM from vitrified GV, fresh GV, vitrified GV 15 per group (*3 experiments) RTqPCR CD9, CD81, DNMT1 , and DNMT3b The expression of all analysed genes was downregulated after IVM of vitrified GV when compared to the fresh in vitro matured MII oocytes. Gao et al [ 125 ] Murine MII after IVM from fresh GV, MII after IVM from vitrified GV, fresh MII, vitrified MII 100 per group RNAseq RTqPCR validation Global analysis (Illumina) Atp5e, Atp5o, Ndufb9, Uqcrq, Timm17a, Dppa5a, H3f3a, Timm13 , and Tomm40 No effect of vitrification on the transcriptome.…”

Section: Impact Of Oocyte Vitrification On Gene Expressionmentioning

confidence: 99%

“…When the analyses focused specifically on genes involved in epigenetic modifications, a significant downregulation of gene expression after vitrification has been consistently reported (Table 4 ) [ 113 , 114 , 129 – 131 , 133 ]. However, Chen et al recently reported an upregulation of two imprinted genes that are known to play a crucial role in development ( Gtl2 and Peg10 ) [ 130 ].…”

Section: Impact Of Oocyte Vitrification On Gene Expressionmentioning

confidence: 99%

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What impact does oocyte vitrification have on epigenetics and gene expression?

Barberet¹,

Barry²,

Choux³

et al. 2020

Clin Epigenet

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Children conceived by assisted reproductive technologies (ART) have a moderate risk for a number of adverse events and conditions. The question whether this additional risk is associated with specific procedures used in ART or whether it is related to the intrinsic biological factors associated with infertility remains unresolved. One of the main hypotheses is that laboratory procedures could have an effect on the epigenome of gametes and embryos. This suspicion is linked to the fact that ART procedures occur precisely during the period when there are major changes in the organization of the epigenome. Oocyte freezing protocols are generally considered safe; however, some evidence suggests that vitrification may be associated with modifications of the epigenetic marks. In this manuscript, after describing the main changes that occur during epigenetic reprogramming, we will provide current information regarding the impact of oocyte vitrification on epigenetic regulation and the consequences on gene expression, both in animals and humans. Overall, the literature suggests that epigenetic and transcriptomic profiles are sensitive to the stress induced by oocyte vitrification, and it also underlines the need to improve our knowledge in this field.

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“…Understanding sustained effects on embryonic gene expression may help to better understand some sublethal cryoinjuries and associated developmental failure. Past studies investigated the effects of oocyte cryopreservation on whole oocyte transcriptomes [34] [35,36] or selected genes [37][38][39][40] and reported significantly altered mRNA levels. Since the MII oocytes are transcriptionally silent [41,42] and major embryonic genome activation occurs at the two-cell stage and four-to eight-cell stage in the mouse [43,44] and human [45], respectively, the published studies do not address how oocytes cryopreservation affect gene expression after embryonic genome activation (EGA) as a way of assessing long-term effects of oocyte cryopreservation.…”

mentioning

confidence: 99%

Probing lasting cryoinjuries to oocyte-embryo transcriptome

Eroglu¹,

Szurek²,

Schall³

et al. 2020

PLoS ONE

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Clinical applications of oocytes cryopreservation include preservation of future fertility of young cancer patients, substitution of embryo freezing to avoid associated legal and ethical issues, and delaying childbearing years. While the outcome of oocyte cryopreservation has recently been improved, currently used vitrification method still suffer from increased biosafety risk and handling issues while slow freezing techniques yield overall low success. Understanding better the mechanism of cryopreservation-induced injuries may lead to development of more reliable and safe methods for oocyte cryopreservation. Using the mouse model, a microarray study was conducted on oocyte cryopreservation to identify cryoinjuries to transcriptionally active genome. To this end, metaphase II (MII) oocytes were subjected to standard slow freezing, and then analyzed at the four-cell stage after embryonic genome activation. Non-frozen four-cell embryos served as controls. Differentially expressed genes were identified and validated using RT-PCR. Embryos produced from the cryopreserved oocytes displayed 200 upregulated and 105 downregulated genes, associated with the regulation of mitochondrial function, protein ubiquitination and maintenance, cellular response to stress and oxidative states, fatty acid and lipid regulation/metabolism, and cell cycle maintenance. These findings reveal previously unrecognized effects of standard slow oocyte freezing on embryonic gene expression, which can be used to guide improvement of oocyte cryopreservation methods.Editor: Joël R. Drevet, chemical toxicity of cryoprotective agents (CPA) [5], osmotic stress [6], disruption of cytoskeleton and spindle microtubules [7,8], premature exocytosis of cortical granules and zona hardening [9, 10], parthenogenetic activation [11][12][13], and polyploidy [7,14,15]. Through intensive efforts to mitigate these cryoinjuries, increasingly encouraging results have been reported with human oocytes after both slow-freezing [16][17][18][19][20][21] and vitrification [22][23][24][25]. A vitrification approach requiring minimum sample volume (less than 1 μl), low permeating CPA concentrations (~30%), and extremely fast cooling/warming rates yielded clinically acceptable results [26][27][28] and is currently the preferred approach for human oocyte cryopreservation. However, the minimal sample volume, low CPA concentrations, and direct contact with LN 2 required to achieve extremely fast cooling/warming rates make this approach prone to devitrification, handling and reproducibility issues, and biosafety risk for contaminating cryopreserved samples with different pathogens [29][30][31][32]. In contrast, slow-freezing methods are usually not associated with a biosafety risk; however, clinical success rates obtained with slowly frozen human oocytes remain lower than those obtained with the vitrification method [19,21,25]. Understanding better the mechanism of cryopreservation-induced injuries may help overcome the shortcomings of the current approaches, and thereby lead to ...

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Expression of CD9 and CD81 in bovine germinal vesicle oocytes after vitrification followed by in vitro maturation

Cited by 11 publications

References 12 publications

Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression

Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression

What impact does oocyte vitrification have on epigenetics and gene expression?

Probing lasting cryoinjuries to oocyte-embryo transcriptome

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