Expression of CD44 variant transcripts in dog lymphatic tissue

Milde, Kerstin F.; Alejandro, Rodolfo; Pastori, Ricardo L.

doi:10.1007/bf00177826

Cited by 7 publications

(7 citation statements)

References 33 publications

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“…In addition to these splice variants, posttranslational modifications by glycosylation and GAG attachments can modify the function of the CD44 isoform (25,26). Thus, it is interesting to consider potential implications of the patterns of similarities and differences in some of the splice variants observed both across tissues of the same species (dog) or between species, such as the rat, dog, and human (27). In recognition of the potential involvement of CD44 variants in the progression of various types of tumors, since CD44 function is ultimately controlled by its posttranslational modifications (28), it is important to question whether or not (and the conditions under which) interspecies differences in CD44 primary structure can influence the accuracy of therapeutic predictions generated with preclinical species.…”

Section: Interstitial Matrix Compositionmentioning

confidence: 99%

Factors Influencing the Use and Interpretation of Animal Models in the Development of Parenteral Drug Delivery Systems

Martinez

2011

AAPS J

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Abstract. Depending upon the drug and drug delivery platform, species-specific physiological differences can lead to errors in the interspecies extrapolation of drug performance. This manuscript provides an overview of the species-specific physiological variables that can influence the performance of parenteral dosage forms such as in situ forming delivery systems, nanoparticles, microspheres, liposomes, targeted delivery systems, lipophilic solutions, and aqueous suspensions. Also discussed are those factors that can influence the partitioning of therapeutic compounds into tumors, the central nervous system and the lymphatics. Understanding interspecies differences in the movement and absorption of molecules is important to the interpretation of data generated through the use of animal models when studying parenteral drug delivery.

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Section: Interstitial Matrix Compositionmentioning

confidence: 99%

Factors Influencing the Use and Interpretation of Animal Models in the Development of Parenteral Drug Delivery Systems

Martinez

2011

AAPS J

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“…The large number of potential CD44 variants and the elevated number of CD44 variant transcripts detectable in normal tissues (21,35,36) raises intriguing questions about their biological role. Several lines of evidence suggest that modifications in the primary sequence of CD44 may influence its known functions (13).…”

Section: Discussionmentioning

confidence: 99%

“…To gain insight into the structure of the CD44 variants, we applied a strategy similar to the one described previously (21). Briefly, PCR reactions were performed with forward primers complementary to exon v3, v7, or v9 and a reverse primer recognizing the CD44 standard exon 17.…”

Section: Expression Of Cd44 Variant Transcripts In Islets Frommentioning

confidence: 99%

“…Total RNA isolation was performed using an RNeasy kit (Qiagen, Chatsworth, CA) following the guidelines recommended by the manufacturer. For cDNA synthesis, standard protocols described elsewhere (21) were applied using a 15-mer oligo(dT) primer and avian myeloblastosis virus-RT (Boehringer Mannheim) for two cycles of cDNA synthesis. All primers used for PCR analyses are in the section below.…”

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confidence: 99%

“…Amplicons were transferred in 20 X SSC to a positively charged nylon membrane (Boehringer Mannheim) and crosslinked to the membrane with ultraviolet light (Stratalinker, Stratagene, La Jolla, CA). The membrane was hybridized at 50°C to dog CD44 standard or variant cDNA probes (21). 32 P or digoxigenin (DIG)-labeled probes were equally used for hybridization.…”

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confidence: 99%

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Expression of a Specific Subset of CD44 Variant Transcripts in NOD Pancreatic Islets

et al. 1996

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Adhesion of lymphocytes to the endothelial venules inside the islets of Langerhans seems to initiate the infiltration of islets in NOD mice. An overexpression of the lymphocyte surface molecule CD44 in infiltrated NOD islets compared with peripheral blood lymphocytes was recently reported. The CD44 protein family includes a variety of molecules generated by alternative RNA splicing from 10 variant exons (v1-v10). By using reverse transcriptase-polymerase chain reaction followed by Southern blotting and hybridization to exon-specific cDNA probes, we investigated the expression of CD44 isoforms in highly purified islets of Langerhans from 4- and 10-week-old NOD mice. At least six CD44 isoforms were strongly overexpressed in NOD islets at 4 and 10 weeks when compared with age-matched BALB/c islets. Controls in different tissues indicate that these variants are specifically increased in the islets from the NOD strain. Islets from the NOD-scid/scid strain also expressed these variant exons. Splenocytes from BALB/c did not express CD44 isoforms, whereas splenocytes from 4-week-old NOD mice did express CD44 variants. Treatment with inflammatory mediators induced new isoforms; however, these transcripts have a different variant exon composition from that found in NOD mice islets. These results suggest that some isoforms are expressed very early in the development of insulitis by a component of the NOD islet itself and underscore a possible role of CD44 in islet infiltration.

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Functional analysis of CD44 variants and xCT in canine tumours

Tanabe

Kimura

Tazawa

et al. 2020

Veterinary Medicine & Sci

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The cell surface glycoprotein CD44 has various types of splicing variants, which contribute to its multiple distinct cellular functions. Recently, it was reported that the CD44v8‐10 isoform interacts with the system Xc(‐) transporter‐related protein (xCT), and inhibits the accumulation of reactive oxygen species by promoting the synthesis of the antioxidant glutathione in human tumour cells. In this study, we investigated the expression and function of CD44 variants and xCT in canine tumours. From semi‐quantitative reverse transcription polymerase chain reaction analysis, the mRNA expression of the CD44v8‐10 isoform was observed in canine tumour tissues as well as human cases. The overexpression of CD44v8‐10 may promote the synthesis of glutathione and enhance the resistance to radiation of canine breast tumour cells. Furthermore, canine xCT mRNA expression was significantly upregulated in the canine breast tumour tissues as compared to the normal tissues surrounding the tumours. To investigate the function of canine xCT, we treated canine tumour cells with the xCT inhibitor sulfasalazine. Consequently, the sulfasalazine‐treated cells were more sensitive to oxidative stress than the non‐treated cells. Taken together, these results suggested that CD44v8‐10 and xCT play important roles in the therapy resistance of canine tumours as well as human tumours.

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Expression of CD44 variant transcripts in dog lymphatic tissue

Cited by 7 publications

References 33 publications

Factors Influencing the Use and Interpretation of Animal Models in the Development of Parenteral Drug Delivery Systems

Factors Influencing the Use and Interpretation of Animal Models in the Development of Parenteral Drug Delivery Systems

Expression of a Specific Subset of CD44 Variant Transcripts in NOD Pancreatic Islets

Functional analysis of CD44 variants and xCT in canine tumours

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