Expression of CD30 mRNA, CD30L mRNA and a variant form of CD30 mRNA in restimulated peripheral blood mononuclear cells (PBMC) of patients with helminthic infections resembling a Th2 disease

Abstract

It has been proposed that CD30, a member of the tumour necrosis factor (TNF) receptor superfamily, is preferentially up-regulated on Th2-type human T cells. In order to investigate a correlation between infection with Echinococcus multilocularis and CD30 expression, we analysed regulation of CD30 mRNA, a variant form of CD30 mRNA (CD30v) and CD30 ligand (CD30L) mRNA expression on PBMC from patients with alveolar echinococcosis (AE) using reverse transcriptase-polymerase chain reaction (RT-PCR). In PBMC of patients with AE as well as healthy donors, spontaneous expression of CD30L mRNA and the CD30v mRNA could be detected. However, the intact form of CD30 mRNA could be detected neither in freshly isolated PBMC of patients nor in PBMC of healthy individuals. Expression of CD30L mRNA and the variant form of CD30 mRNA was frequently detected at individual time points during 72 h of culture of PBMC stimulated with crude Echinococcus antigen. In contrast to CD30v or CD30L mRNA expression, induction of CD30 mRNA expression was detected only in three out of six (50%) healthy donors and in 10 out of 21 (48%) patients with alveolar echinococcosis after 72 h of incubation. As a control, mitogenic stimulation of PBMC of both healthy individuals and infected patients led to expression of intact CD30 mRNA within 24 h of culture. These data demonstrate the different expression of two different forms of CD30 mRNA in PBMC of human individuals. The specific induction of CD30 expression is correlated only in rare cases with the clinical status of patients with AE, indicating the lack of a general induction of CD30 mRNA in this Th2-type-dominated helminthic disease. The data provide further evidence that the CD30 receptor is not an exclusive marker for a Th2-type response.

CD30+ T Cells in Rheumatoid Synovitis: Mechanisms of Recruitment and Functional Role

“…An alternative explanation may include the presence of transcription inhibitory factors within the SM or the lack/downmodulation of adhesion molecules able to anchor CD30 ϩ cells to the inflamed tissue (29). Our mRNA data are different from those of other researchers who failed to detect CD30 transcript in PBL (30). We believe that this discrepancy is due to the far more sensitive method used in our study.…”

Frequent Expression of the Variant CD30 in Human Malignant Myeloid and Lymphoid Neoplasms

“…However, our data are of some interest because the simultaneous expression of CD30v and CD30L has been noted, in the absence of CD30, in normal peripheral blood T cells from patients affected by helminthic infections. 36 Moreover, the parallel up-regulation of CD30v-and CD30L-specific transcripts was also observed after in vitro activation of peripheral blood mononuclear cells from healthy donors by helminthic antigens. 36 Finally, high levels of constitutive CD30v and CD30L mRNA and protein were noted in normal peripheral blood neutrophils (Gattei et al, unpublished observation).…”

“…36 Moreover, the parallel up-regulation of CD30v-and CD30L-specific transcripts was also observed after in vitro activation of peripheral blood mononuclear cells from healthy donors by helminthic antigens. 36 Finally, high levels of constitutive CD30v and CD30L mRNA and protein were noted in normal peripheral blood neutrophils (Gattei et al, unpublished observation). 10 Interestingly, reverse signaling via CD30L was observed in freshly isolated neutrophils and T cells suboptimally stimulated by anti-CD3, leading to production of interleukin-8 and a rapid oxidative burst, or to an increased metabolic activity, proliferation, and production of interleukin-6, respectively.…”

Extremely Rapid and Intense Induction of Apoptosis in Human Eosinophils by Anti-CD30 Antibody Treatment In Vitro

“…Soluble levels of CD30 are elevated and are associated with the prognosis in Hodgkin's disease and AIDS (38). Elevated levels were also reported in patients with adult T cell leukemia and some of those with non-Hodgkin's lymphoma, rheumatoid arthritis (39), chronic renal failure with primary growth retardation (40), atopic dermatitis (41)(42)(43), bullous pemphigoid (44), polymyositis/dermatomyositis (45), and helminth infection (46). Interestingly, some of these diseases are associated with eosinophilia (47)(48)(49)(50), and an especially positive correlation between the levels of soluble CD30 (sCD30) and eosinophilia was found in Hodgkin's disease (51).…”