Expression of CD24, P-cadherin and S100A4 in tumors of the ampulla of Vater

Abstract

Carcinomas of the Vaterian system are rare and presumably arise from preexisting adenomas (adenomacarcinoma-sequence). Usually, biopsies are obtained to confirm and specify endoscopic findings, but differentiating reactive atypia from dysplasia or dysplasia from invasive carcinoma can sometimes be difficult or even impossible on morphological criteria alone. In case of invasive carcinoma, furthermore, the precise classification of carcinoma subtypes needs to be established since the distinct subtypes differ significantly in terms of clinical outcome. The cell adhesion proteins CD24, P-cadherin and S100A4 were shown to be expressed in several carcinomas and in dysplastic epithelium but only rarely in normal mucosa. We therefore investigated their expression in 177 carcinoma, 114 adenoma and 152 normal mucosa specimens of the ampulla of Vater. Although the expression of the cell adhesion proteins did not differ between the carcinoma subtypes, marked differences between normal mucosa, adenoma and carcinoma samples were observed. All marker proteins were expressed in less than 7% of normal mucosa samples (S100A4 in only 1% of cases) and showed an increasing expression from adenoma to invasive carcinoma. Our findings suggest that P-cadherin and S100A4 are helpful in discriminating normal mucosa or reactive atypia from neoplastic lesions. CD24 and S100A4, furthermore, can assist in the differential diagnosis of dysplasia vs invasive carcinoma.

Immunohistochemical Expressionof S100a4 and Cd24 in Colorectal Adenoma and Carcinoma.

“…Whereas some authors claim that normal colonic mucosa didn't express CD 24 (27).Others were able to detect CD24 expression in normal mucosa (13,28).In this work, the adjacent non-neoplastic mucosa displayed weak CD24 membranous staining in only four cases of CRC.…”

“…Nearly similar observations were reported by Choi et al (26). Baumhoer et al (13) reported absent CD24 expression in dysplastic lesions. In contrast, Sagiv et al (28) reported that CD24 was highly expressed in both cases of adenoma and carcinoma.…”

“…In our work, S100A4 was only observed in high grade adenomas.Baumhoer et al (13) reported positive S100A4 expression in 11% of the studied cases of adenomas. Zhao et al (7) reported S100A4 expression in 23.3% of the studied cases of gastric dysplasia.…”

“…The pattern of CD24 expression was evaluated as membranous and/or cytoplasmic staining while cytoplasmic staining was considered positive for S100A4.S100A4 and CD24 expression was scored semiquantitatively according to the percentage of positive cells as follows: 0, negative, 1+, minimal (<10%) positive cells; 2+, moderate (10-50%) positive cells; and 3+, diffuse/marked (>50%) positive cells (13). For statistical purpose, only cases with scores 2 or 3 were considered positive cases.The staining intensity was scored as follows: negative, weak, moderate, and strong.…”

The role of adhesion molecules in acute myeloid leukemia and (hemato)oncology: A systematic review

“…Bearing of the CLA epitope (CLA, cutaneous lymphocyte-associated antigen) is associated with the acquisition of both P-selectin and E-selectin ligand functions 36 . New insights on adhesive interactions and the prognostic relevance of tumor CD24 expression have been recently documented for various nonhematological malignancies [37][38][39][40][41][42][43] . Knowledge of sLe X mediated adhesive interactions with endothelial cells and platelets is important for understanding the pathophysiology of tumor metastasis [44][45][46][47][48] .…”