Expression of CCT6A mRNA in chicken granulosa cells is regulated by progesterone

Wei, Qingqing; Zhu, Guiyu; Cui, Xinxing; Li, Kang; Cao, Deliang; Jiang, Yunliang

doi:10.1016/j.ygcen.2013.04.019

Cited by 13 publications

(19 citation statements)

References 52 publications

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“…In the present study, HSPD1 and CCT6A were found to be a possible target-related protein of SCU in proteomic analysis of cytoplasm fraction and EIF6 was found to be a possible target-related protein of SCU in analysis of nuclei/membrane fraction. Our results were consistent with the reported subcellular location of HSPD1 in mitochondria [ 38 , 39 ], CCT6A in cytoplasm [ 37 ], and nucleocytoplasmic shuttling of EIF6 [ 40 , 41 ]. Interestingly, as shown in the network of bioinformatic analysis, EIF6 had direct interactions with HSPD1 and CCT6A.…”

Section: Discussionsupporting

confidence: 92%

“…CCT6A is the zeta subunit of the chaperonin containing TCP1 complex and is the only cytosolic chaperonin in eukaryotes. It was estimated to assist in the folding of multiple proteins such as actin, tubulin, cyclin E, myosin, and transducin [ 37 ]. Our finding about the increase levels of HSPD1 and CCT6A in SCU-treated cells suggested that induction of expression of chaperonin proteins might be involved in the protective effects of SCU against HR injury.…”

Section: Discussionmentioning

confidence: 99%

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Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins

Shi

Liu

Ling

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Scutellarin (SCU) is one of the main components of traditional Chinese medicine plant Erigeron breviscapus (Vant.) Hand.-Mazz. In this paper, we studied the protective effects of SCU on human cardiac microvascular endothelial cells (HCMECs) against hypoxia-reoxygenation (HR) injury and its possible target-related proteins. Results of MTT assay showed that pretreatment of SCU at doses of 1, 5, and 10 μM for 2 h could significantly inhibit the decrease in cell viability of HCMECs induced by HR injury. Subcellular fractions of cells treated with vehicle control, 1 μM SCU, HR injury, or 1 μM SCU + HR injury were separated by ultracentrifugation. The protein expression profiles of cytoplasm and membrane/nuclei fractions were checked using protein two-dimensional electrophoresis (2-DE). Proteins differentially expressed between control and SCU-treated group, control and HR group, or HR and SCU + HR group were identified using mass spectrometry (MS/MS). Possible interaction network of these target-related proteins was predicted using bioinformatic analysis. The influence of SCU on the expression levels of these proteins was confirmed using Western blotting assay. The results indicated that proteins such as p27BBP protein (EIF6), heat shock 60 kDa protein 1 (HSPD1), and chaperonin containing TCP1 subunit 6A isoform (CCT6A) might play important roles in the effects of SCU.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins

Shi

Liu

Ling

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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“…Chaperonin containing T-complex polypeptide 1 (CCT6A) is the zeta subunit of the chaperonin containing TCP1 complex (CCT). This complex is composed of two similar rings, each containing eight different proteins (alpha, beta, gamma, delta, epsilon, eta, theta and zeta; for review: [ 62 ]). Each subunit of the ring has three domains: an ATP binding site, an unfolded protein binding domain and a region which connects the two other regions.…”

Section: Resultsmentioning

confidence: 99%

Buserelin alleviates chloride transport defect in human cystic fibrosis nasal epithelial cells

et al. 2017

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Cystic fibrosis (CF) is the most common autosomal recessive disease in Caucasians caused by mutations in the gene encoding the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) chloride (Cl-) channel regulated by protein kinases, phosphatases, divalent cations and by protein-protein interactions. Among protein-protein interactions, we previously showed that Annexin A5 (AnxA5) binds to CFTR and is involved in the channel localization within membranes and in its Cl- channel function. The deletion of phenylalanine at position 508 (F508del) is the most common mutation in CF which leads to an altered protein (F508del-CFTR) folding with a nascent protein retained within the ER and is quickly degraded. We previously showed that AnxA5 binds to F508del-CFTR and that its increased expression due to a Gonadoliberin (GnRH) augments Cl- efflux in cells expressing F508del-CFTR. The aim of the present work was to use the GnRH analog buserelin which is already used in medicine. Human nasal epithelial cells from controls and CF patients (F508del/F508del) were treated with buserelin and we show here that the treatment alleviates Cl- channel defects in CF cells. Using proteomics we highlighted some proteins explaining this result. Finally, we propose that buserelin is a potential new pharmaceutical compound that can be used in CF and that bronchus can be targeted since we show here that they express GnRH-R.

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“…In another study, we found a significant higher expression of CCT6A mRNA in theca cells than in granulosa cells of chicken ovarian follicle, and in theca cells, knock-down of CCT6A decreased Bcl-2 expression (Yang et al, 2015), suggesting that CCT6A likely affect egg production by stimulating the proliferation of theca cells. Wei et al (2013) found that progesterone can activate CCT6A transcription by the progesterone response element (PRE) located from -2056 to -2051 of chicken CCT6A gene promoter. The two mutations at -2215 and -1959 sites reported by this study were located at the 5′ -and 3′ -flanking regions of PRE of the distal promoter of chicken CCT6A gene, respectively; therefore, may interfere the binding of PGR or other transcriptional factors to the promoter, or interact with other polymorphisms regulating CCT6A gene expression, which requires further studies.…”

Section: Discussionmentioning

confidence: 99%

“…Up to date, polymorphisms in the chicken CCT6A gene and its association with egg production traits were not reported. Due to the fact that the tempospatial expression of chicken CCT6A is consistent with sexual maturation, follicle growth and ovary function, and that deletion from -2534 to -1549 of chicken CCT6A gene promoter region cause up-regulation in luciferase activity (Wei et al, 2013), therefore, in this study, we set out to find polymorphisms in this promoter region of chicken CCT6A and further analyze their genetic effects on egg production traits and mRNA expression level.…”

Section: Introductionmentioning

confidence: 99%

Identification and Genetic Effect of Haplotypes in the Distal Promoter Region of Chicken <i>CCT6A </i>Gene Associated with Egg Production Traits

Yang

Wei

et al. 2016

J. Poult. Sci.

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The chaperonin containing TCP-1 complex protein 1 subunit zeta (CCT6A) is the only cytosolic chaperonin in eukaryotes assisting in the folding of cytoplasmic proteins. Previous study revealed that the mRNA expression of chicken CCT6A gene was remarkably elevated in the sexually mature ovaries. However, the mechanism underlying chicken CCT6A expression changes remains largely unknown. In this study, haplotypes caused by two single nucleotide polymorphisms ( ) were detected in all of the five chicken populations. Diplotypes AA, AD and DD were predominant in Xinyang brown hens, among which diplotype AD was associated with higher egg number at the age of 28 weeks old (E28) (P ＜0.05). In addition, diplotype AD was also predominant in Xinyang brown and Hy-line brown chicken populations with high egg production; whereas in Wenchang and Shouguang chicken populations which are Chinese indigenous chicken breeds and relatively lower in egg production, diplotype AA was predominant. Compared with diplotypes AA and DD, the mRNA expression of CCT6A in diplotype AD birds is the highest in F1, F5, and POF1 follicles of Hy-line brown hens (P＜0.05). These results suggest that the two SNPs in chicken CCT6A promoter region are potential DNA marker for improving egg production trait.

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Expression of CCT6A mRNA in chicken granulosa cells is regulated by progesterone

Cited by 13 publications

References 52 publications

Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins

Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins

Buserelin alleviates chloride transport defect in human cystic fibrosis nasal epithelial cells

Identification and Genetic Effect of Haplotypes in the Distal Promoter Region of Chicken <i>CCT6A </i>Gene Associated with Egg Production Traits

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