Expression of CCL27 in Middle Ear Cholesteatoma

Abstract

BACKGROUND: Cholesteatoma is a lesion of the temporal bone lined by stratified squamous epithelium that contains desquamated keratin. Cholesteatoma is considered more aggressive during childhood. The molecular mechanism of the pathogenesis of cholesteatoma formation is still unclear. Previous studies reported on immunohistochemical examination and quantitative polymerase chain reaction (PCR) found that TCN1 and CCL27 were involved in the process of cholesteatoma keratinocytes and dermal endothelial cells. In skin tumors derived from keratinocytes, there is a protective mechanism of antitumor T cell-mediated by reducing CCL27 expression. AIM: The objective of this study was to investigate the expression of chemokine ligand 27 (CCL27) in cholesteatoma. METHODS: This is a cross-sectional comparative study. Cholesteatoma specimens were obtained from 15 patients who underwent surgery and 15 normal retroauricular skin as control. The specimen’s gene expression was examined with real-time PCR (RT-PCR). RESULTS: The expression of CCL27 was 36.215 ± 45.848 ng/ul in cholesteatoma, while it is 9.692 ± 15.760 ng/ul in normal retroauricular skin. The expression of CCL 27 in cholesteatoma was higher than in normal retroauricular skin, but it was not significant (p > 0.05) CONCLUSION: The expression of CCL27 in cholesteatoma was higher than in normal retroauricular skin.