Expression of caveolin‐1 in the rat temporomandibular joint

Nozawa-Inoue, Kayoko; Suzuki, Akiko; Amizuka, Norio; Maeda, Takeyasu

doi:10.1002/ar.a.20275

Cited by 4 publications

(3 citation statements)

References 25 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1d). Almost all the type B cells showed caveolin‐1 immunoreactions (Nozawa‐Inoue et al, 2006; present study), while a few of them lacked caveolin‐3 immunoreactivity in the lining cell layer (Fig. 1d).…”

Section: Resultssupporting

confidence: 56%

“…The type A cell is characterized by numerous vacuoles, lysosomes, nuclei with rich heterochromatin, and cell surface filopodia, while the type B cell possesses a well‐developed rough endoplasmic reticulum (rER), a nucleus with less heterochromatin, long cytoplasmic projections, surrounding basement membrane‐like structure in addition to numerous flask‐shaped invaginations in the cell membrane (Nozawa‐Inoue et al, 1999, 2003). A recent immunoelectron microscopic investigation showed these flask‐shaped structures to be intensely immunoreacted with caveolin‐1, suggesting that they were caveolae (Nozawa‐Inoue et al, 2006).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Expression of caveolin‐3 in the fibroblast‐like type B synoviocytes in the rat temporomandibular joint

Nozawa-Inoue

Suzuki

Niwano

et al. 2007

The Anatomical Record

Self Cite

View full text Add to dashboard Cite

The present study revealed that the fibroblast-like type B synoviocytes (covering the surface of the synovial membrane in the rat temporomandibular joint) had muscle-specific caveolin-3 protein in their caveolae. The existence of two kinds of type B synoviocytes (with and without caveolin-3-immunoreactions even in the synovial lining layer) might reflect the functional difference between them. Anat Rec, 290:238-242, 2007. 2007 Wiley-Liss, Inc.

show abstract

Section: Resultssupporting

confidence: 56%

mentioning

confidence: 99%

Expression of caveolin‐3 in the fibroblast‐like type B synoviocytes in the rat temporomandibular joint

Nozawa-Inoue

Suzuki

Niwano

et al. 2007

The Anatomical Record

Self Cite

View full text Add to dashboard Cite

show abstract

“…We confirmed an intense expression of caveolin‐1 in the caveolae of the type B cell by immunocytochemistry using an antibody to the 25‐kDa heat shock protein (Hsp25) that is a marker of the type B cells (Nozawa‐Inoue et al. , ). Interestingly, the caveolin‐1‐positive caveolae additionally exhibited muscle‐specific caveolin‐3‐reactivity in some of the type B cells (Nozawa‐Inoue et al.…”

Section: Introductionsupporting

confidence: 63%

Contribution of synovial lining cells to synovial vascularization of the rat temporomandibular joint

et al. 2015

Self Cite

View full text Add to dashboard Cite

The lining layer of the synovial membrane in the temporomandibular joint (TMJ) contains two types of lining cells: macrophage-like type A and fibroblast-like type B cells. The type B cells are particularly heterogeneous in their morphology and immunoreactivity, so that details of their functions remain unclear. Some of the type B cells exhibit certain resemblances in their ultrastructure to those of an activated capillary pericyte at the initial stage of the angiogenesis. The articular surface, composed of cartilage and the disc in the TMJ, has few vasculatures, whereas the synovial lining layer is richly equipped with blood capillaries to produce the constituent of synovial fluid. The present study investigated at both the light and electron microscopic levels the immunocytochemical characteristics of the synovial lining cells in the adult rat TMJ, focusing on their contribution to the synovial vascularization. It also employed an intravascular perfusion with Lycopersicon esculentum (tomato) lectin to identify functional vessels in vivo. Results showed that several type B cells expressed desmin, a muscle-specific intermediate filament which is known as the earliest protein to appear during myogenesis as well as being a marker for the immature capillary pericyte. These desmin-positive type B cells showed immunoreactions for vimentin and pericyte markers (neuron-glial 2; NG2 and PDGFRβ) but not for the other markers of myogenic cells (MyoD and myogenin) or a contractile apparatus (αSMA and caldesmon). Immunoreactivity for RECA-1, an endothelial marker, was observed in the macrophage-like type A cells. The arterioles and venules inside the synovial folds extended numerous capillaries with RECA-1-positive endothelial cells and desmin-positive pericytes to distribute densely in the lining layer. The distal portion of these capillaries showing RECA-1-immunoreactivity lacked lectin-staining, indicating a loss of blood-circulation due to sprouting or termination in the lining layer. The desmin-positive type B and RECA-1-positive type A cells attached to this portion of the capillaries. Some capillaries in the lining layer also expressed ninein, a marker for sprouting endothelial cells, called tip cells. Since an activated pericyte, macrophage and tip cell are known to act together at the forefront of the vessel sprout during angiogenesis, the desmin-positive type B cell and RECA-1-positive type A cell might serve as these angiogenic cells in the synovial lining layer. Tomato lectin perfusion following decalcification would be a highly useful tool for research on the vasculature of the mineralized tissue. Use of this technique combined with immunohistochemistry should permit future extensive investigations on the presence of the physiological angiogenesis and on the function of the lining cells in the synovial membrane.

show abstract

Immunocytochemical Localization of Caveolin‐3 in the Synoviocytes of the Rat Temporomandibular Joint During Development

Niwano¹,

Nozawa-Inoue²,

Suzuki³

et al. 2008

The Anatomical Record

Self Cite

View full text Add to dashboard Cite

Caveolins-caveolin-1, -2, -3 (Cav1, 2, 3)-are major components of caveolae, which have diverse functions. Our recent study on the temporomandibular joint (TMJ) revealed expressions of Cav1 and muscle-specific Cav3 in some synovial fibroblast-like type B cells with well-developed caveolae. However, the involvement of Cav3 expression in the differentiation and maturation of type B cells remains unclear. The present study therefore examined the chronological alterations in the localization of Cav3 in the synovial lining cells of the rat TMJ during postnatal development by immunocytochemical techniques. Observations showed immature type B cells possessed a few caveolae with Cav1 but lacked Cav3 protein at postnatal day 5 (P5). At P14, Cav3-immunopositive type B cells first appeared in the synovial lining layer. They increased in number and immunointensity from P14 to P21 as occlusion became active. In immunoelectron microscopy and double immunolabeling with heat shock protein 25 (Hsp25) and Cav3, coexpressed type B cells developed rough endoplasmic reticulum and numerous caveolae, while the Cav3-immunonegative type B cell with Hsp25 immunoreaction possessed few of these. Results suggest that Cav3 expression, which is closely related to added functional stimuli, reflects the differentiation of the type B synoviocytes. Anat Rec,

show abstract

Expression of caveolin‐1 in the rat temporomandibular joint

Cited by 4 publications

References 25 publications

Expression of caveolin‐3 in the fibroblast‐like type B synoviocytes in the rat temporomandibular joint

Expression of caveolin‐3 in the fibroblast‐like type B synoviocytes in the rat temporomandibular joint

Contribution of synovial lining cells to synovial vascularization of the rat temporomandibular joint

Immunocytochemical Localization of Caveolin‐3 in the Synoviocytes of the Rat Temporomandibular Joint During Development

Contact Info

Product

Resources

About