Expression of Caveolin-1, Caveolin-2 and Caveolin-3 in Thyroid Cancer and Stroma

Kim, Dokyung; Kim, Haeryoung; Koo, Ja Seung

doi:10.1159/000329472

Cited by 18 publications

(24 citation statements)

References 75 publications

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“…Caveolin is the main structural protein of caveolae and could be divided into three subtypes as Cav-1, caveolin-2 and caveolin-3 [31]. Among them, Cav-1 is most prominently expressed in vascular ECs through which eNOS is targeted to caveolae by cotranslational N -myristoylation and posttranslational palmitoylation [11].…”

Section: Discussionmentioning

confidence: 99%

Caveolae Depletion Contributes to Vasorelaxant Effects of Chenodeoxycholic Acid

Wang¹,

Lv²,

Liu³

et al. 2017

Cell Physiol Biochem

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Background/Aims: High concentration of bile acids (BAs) induces hydrophobicity-dependent vasorelaxtant effects with hydrophobic BAs showing greater responses than hydrophilic BAs, of which the underlying mechanisms are still unclear. Caveolae are invaginations on membranes of endothelial cells (ECs) entraping endothelial nitric oxide synthase (eNOS) to prevent its activation, which plays a critical role in regulation of vascular function. The purpose of the present study was to investigate the role of caveolae in vasorelaxant effects of BAs. Methods: Chenodeoxycholic acid (CDCA) and cholic acid (CA) were used to represent hydrophobic and hydrophilic BA, respectively. Vascular responses of abdominal aorta were measured by isometric force recording. Morphology of caveolae was examined by transmission electron microscopy. Protein expression of total eNOS (t-eNOS) or phosphorylated eNOS (p-eNOS) was determined by Western blot. Nitric oxide (NO) content was observed by fluorometric assay. Results: We demonstrated that CDCA as well as Methyl-β-cyclodextrin (MCD), a commonly used reagent for cholesterol depletion, reduced potassium chloride (KCl)- or phenylephrine (PE)-elicited vasoconstriction (P < 0.05), and enhanced acetylcholine (Ach)-elicited vasodilatation (P < 0.05) in endothelium-intact abdominal aorta but not in endothelium-denuded or CA-treated vessels. CDCA and MCD, but not CA significantly disrupted caveolae structure on ECs of abdominal aorta which was recovered by cholesterol incubation (P < 0.05). Protein expression of t-eNOS was significantly decreased (P < 0.05), and that of p-eNOS together with NO content was significantly increased in CDCA- and MCD- but not CA-treated vessels (P < 0.05) as compared with vehicle. The effect was reversed by either endothelium-denudation or cholesterol replenishment. Moreover, with cholesterol incubation, no significant differences were found in vascular responses among CDCA-, CA- or vehicle-treated vessels. Conclusion: These results indicate that CDCA diminishes caveolae on ECs of abdominal aorta promoting eNOS phosphorylation and NO production which contributes to its vasorelaxtant effect.

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Section: Discussionmentioning

confidence: 99%

Caveolae Depletion Contributes to Vasorelaxant Effects of Chenodeoxycholic Acid

Wang¹,

Lv²,

Liu³

et al. 2017

Cell Physiol Biochem

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“…5). Caveolins are expressed in thyroid cancer (23, 64) and other tumors, but little is known about their function in the thyroid. In prostate cancer cells, CAV1 is associated with disease progression (65).…”

Section: Discussionmentioning

confidence: 99%

Gravity‐sensitive signaling drives 3‐dimensional formation of multicellular thyroid cancer spheroids

et al. 2012

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This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-κB p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative real-time PCR using the same samples. Compared with 1-g IL-6, IL-8, CD44, and OPN were significantly up-regulated in AD cells but not in MCTSs, while ERK1/2, CAV2, TLN1, and CTGF were significantly down-regulated in AD cells. Simultaneously, the expression of ERK2, IL-6, CAV2, TLN1, and CTGF was reduced in MCTSs. IL-6 protein expression and secretion mirrored its gene expression. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-κB p65 in RPM-dependent thyroid carcinoma cell spheroid formation.

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“…28 In our study, caveolin-1-positive expression was significantly increased in PTC tissues as revealed by immunohistochemistry staining (w 2 ¼ 15.643, P ¼ .001), in agreement with previous studies. 18,19 Unexpectedly, we found that the solid tissue of BA had higher caveolin-1 expression, whereas solid tumor tissue of PTC had lower caveolin-1 expression, accompanied by downregulation of caveolin-1 expression in para-tumor tissue compared to in normal thyroid tissue as evidenced by Western blot analysis (Figure 2). Therefore, the change in caveolin-1 expression in different thyroid tissues may be like the changes in insulin levels in different phases during the development of type 2 diabetes mellitus.…”

Section: Discussionmentioning

confidence: 70%

Expression of Caveolin-1 Is Associated With Thyroid Function in Patients With Human Papillary Thyroid Carcinoma

Zhang

Yan

et al. 2020

Dose-Response

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Objective: The aim of this study was to evaluate the levels of caveolin-1 in thyroid follicular epithelial cells of papillary thyroid cancer, follicular thyroid cancer, and nonmalignant thyroid nodule benign follicular adenoma, as well as to explore the relationship between the levels of caveolin-1 and thyroid function. Methods: Thirty cases of papillary thyroid cancer, 10 cases of follicular thyroid cancer, 32 cases of nonmalignant thyroid nodule benign follicular adenoma, and 30 controls were enrolled in this study. Caveolin-1 expression in tissue specimens obtained from these cases was evaluated by immunohistochemistry and Western blotting. Results: Caveolin-1 expression in thyroid epithelial cells of patients with papillary thyroid cancer, particularly female patients, was significantly higher than that in patients with follicular thyroid cancer and nonmalignant thyroid nodule benign follicular adenoma ( P < .005). Serum thyroid-stimulating hormone (TSH) levels in the caveolin-1-positive expression group were lower than that in the caveolin-1-negative expression group, and the lowest expression of caveolin-1 was detected in tissues of patients with Graves’ disease. The serum TSH level was associated with caveolin-1 expression in thyroid epithelial cells. Conclusion: Caveolin-1 may participate in regulating thyroid function and is a potential biomarker of follicular thyroid cancer.

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Expression of Caveolin-1, Caveolin-2 and Caveolin-3 in Thyroid Cancer and Stroma

Cited by 18 publications

References 75 publications

Caveolae Depletion Contributes to Vasorelaxant Effects of Chenodeoxycholic Acid

Caveolae Depletion Contributes to Vasorelaxant Effects of Chenodeoxycholic Acid

Gravity‐sensitive signaling drives 3‐dimensional formation of multicellular thyroid cancer spheroids

Expression of Caveolin-1 Is Associated With Thyroid Function in Patients With Human Papillary Thyroid Carcinoma

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