Expression of CASC9 and LINC01234 in Different Cell Lines

Abstract

Cancers are multifactorial but the advancement in research in the recent decades have drawn attention towards lncRNAs as they are proven to play important function as biological regulators. LINC01234, CASC9 are important long non coding RNAs (lncRNAs). They are not extensively studied therefore, their role in esophageal cancer as well as in other cancers is still not clear. There are few studies about LINC01234 and CASC9 for their role in esophageal cancer. The objective of the study was to investigate the expression of LINC01234 and CASC9 in different cell lines. Our lab cultured and maintained model esophageal cancer cells which were obtained after transformation of human embryonic esophageal epithelial cells into malignant cells by HPV18 E6E7. After this, elevated expression of LINC01234 was found in Gene Chip showing that it may have some role in causing esophageal cancer. For further verification, qRT-PCR was used for the detection of expression level of LINC01234 in different cell lines. Furthermore, expression of CASC9 was also investigated by qRT-PCR in different cell lines. Expression of LINC01234 and CASC9 was markedly high in esophageal cancer cell lines as compared to other cell lines while high expression of LINC01234 was also found in one lung cancer cell line. These lncRNAs may be used as biomarker and potential therapeutic target for esophageal cancer intervention by further exploration of their functions.