Expression of carbonic anhydrase isoenzymes IV and II in rat epididymal duct

Kaunisto, Kari; Parkkila, Seppo; Parkkila, Anna‐Kaisa; Waheed, Abdül; Sly, William S.; Rajaniemi, Hannu

doi:10.1095/biolreprod52.6.1350

Cited by 51 publications

(50 citation statements)

References 44 publications

(52 reference statements)

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“…This could indicate the presence of soluble CAII in the lumen, which attaches to the apical membrane of all cells, or it could indicate some degree of crossreactivity of the antibody with another CA isoform. CAIV has been previously shown to be located on apical membranes in this region of the epididymis (Kaunisto et al 1995), but the anti-CAII antibody used in our study does not cross react with CAIV in the kidney (Breton et al 1995).…”

Section: Discussioncontrasting

confidence: 49%

“…was used at a 1:100 dilution in PBS/azide. The use of these antibodies has been previously characterized in rat kidney and epididymis (Breton et al 1995Brown et al 1992;Kaunisto et al 1995). The secondary antibody used was a polyclonal goat anti-rabbit IgG, conjugated to FITC (Kirkegaard and Perry, Gaithersburg, Md.)…”

Section: Antibodiesmentioning

confidence: 99%

“…These cells are involved in luminal acidification, in the vas deferens, and they contain high levels of the enzyme carbonic anhydrase type II (CAII; Breton et al 1996). Apical and narrow cells in the caput epididymidis are also CAII positive, but the presence of CA in clear cells is controversial, since conflicting data have been reported (Cohen et al 1976;Kaunisto et al 1995;Membre et al 1985).…”

Section: Introductionmentioning

confidence: 99%

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Postnatal development of H + ATPase (proton-pump)-rich cells in rat epididymis

Breton

Tyszkowski²,

Sabolić

et al. 1999

Histochemistry and Cell Biology

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Active proton secretion and bicarbonate reabsorption by epithelial cells of the mammalian excurrent duct system maintains an acidic luminal pH that is involved in creating a suitable environment for sperm maturation and storage. Both an apical Na/H exchanger and an apical H+ ATPase have been implicated in luminal acidification. The H+ ATPase is located in apical and/or narrow cells in the caput epididymidis, and clear cells in the corpus and cauda epididymidis. As a step toward understanding the acute and chronic regulation of luminal acidification in excurrent ducts, we have followed the appearance of H+ ATPase-rich cells in rat epididymis during postnatal development, using antibodies to subunits of the H+ ATPase. In addition, we performed double staining with antibodies against carbonic anhydrase type II (CAII). H+ ATPase-rich cells were already detectable 2 weeks after birth in all regions of the epididymis, and reached maximum numbers after 3-4 weeks. CAII-rich cells followed a similar developmental pattern. In adult rats, the number of H+ ATPase/CAII-positive cells in the cauda was on average more than double the number in the caput epididymidis, although considerable intertubule variability was seen in both regions. Double immunostaining showed that CAII and H+ ATPase were colocalized in the same cells in the caput and cauda, but H+ ATPase-rich cells in the corpus contained low levels of CAII. These results demonstrate that differentiated subpopulations of proton-secreting epithelial cells appear early during epididymal development, and that the induction of H+ ATPase in these cells occurs prior to sexual maturation.

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Section: Discussioncontrasting

confidence: 49%

Section: Antibodiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Postnatal development of H + ATPase (proton-pump)-rich cells in rat epididymis

Breton

Tyszkowski²,

Sabolić

et al. 1999

Histochemistry and Cell Biology

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“…CA isoenzymes participate in pH homeostasis in various biological fluids [Maren, 1967;Tashian, 1989;Brown et al, 1990;Swenson, 1991;Tashian, 1992;Kaunisto et al, 1995;Sly and Hu, 1995;Parkkila and Parkkila, 1996;Lai et al, 1998]. Since dental caries is a consequence of a decrease in pH on tooth surfaces, we undertook to investigate the relationship of salivary CA VI concentration to the occurrence of caries.…”

Section: Introductionmentioning

confidence: 99%

A Low Concentration of Carbonic Anhydrase Isoenzyme VI in Whole Saliva Is Associated with Caries Prevalence

Kivelä

Parkkila²,

Parkkila³

et al. 1999

Caries Res

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Carbonic anhydrases maintain pH homeostasis in various tissues of the human body by catalyzing the reversible reaction CO₂ + H₂O <=> HCO₃^– + H⁺. Carbonic anhydrase isoenzyme VI (CA VI) is secreted into human saliva by the serous acinar cells of the parotid and submandibular glands. Although it represents about 3% of the total protein in stimulated parotid saliva, its exact physiological significance in the saliva has not been established. In the present study, saliva samples were collected under strictly controlled conditions from young, healthy men and assayed for CA VI concentrations using a specific time–resolved immunofluorometric assay. Salivary secretion rate, pH, buffering capacity, α–amylase activity levels, lactobacillus and Streptococcus mutans counts were also determined, and the results were correlated with the dental status of the subjects. Salivary CA VI concentration, pH and buffering capacity values correlated negatively with the numbers of decayed, missing and filled teeth (DMFT index). The correlations between salivary CA VI concentration and DMFT index were most significant in subjects with poor oral hygiene. No correlation was found between salivary CA VI concentration and lactobacillus or Streptococcus mutans counts. As predicted, salivary lactobacillus and Streptococcus mutans counts showed a close positive correlation with the DMFT index. In contrast, no significant correlation was seen between salivary secretion rate or amylase activity and the DMFT index. The present results indicate that low salivary CA VI concentrations are associated with increased caries prevalence, particularly in subjects with neglected oral hygiene.

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“…CA activity in the male reproductive tract might be causally related to the quiescence (immobile) state of stored spermatozoa by regulating the acid±base balance of the luminal uid [15]. This condition exists in the intact rat epididymis, whereby luminal¯uid becomes more acidic as it¯ows from the caput to the cauda epididymis.…”

mentioning

confidence: 99%