Expression of Cancer-Testis Genes in Brain Tumors

Lee, Myoung-Hee; Son, Eun-Ik; Kim, Ealmaan; Kim, In-Soo; Yim, Man-Bin; Kim, Sang-Pyo

doi:10.3340/jkns.2008.43.4.190

Cited by 16 publications

(14 citation statements)

References 20 publications

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“…25 A recent study by Lee, however, did not demonstrate any SCP-1 expression in meningiomas, and instead demonstrated 85% and 35% of MAGE-E1 and SOX-6 expression, respectively. 28 We did not study these two antigens, but we also did not find any SCP-1 expression. We found three of 26 (12%) expression of NY-ESO-1 in meningioma tissue.…”

Section: Discussionmentioning

confidence: 76%

“…4,23,25,28,[45][46][47][48] To date, a comprehensive expression analysis of both CDA and MDA in glioma is lacking. This study demonstrates by use of the RT-PCR technique that malignant gliomas express particular CTA and MDA.…”

Section: Discussionmentioning

confidence: 99%

“…1,4,18,[25][26][27][28][29] To understand the degree of variability in brain tumors, we analyzed the composite expression of 11 CTA and four MDA in malignant glioma tissue and primary glioma cell lines and compared them with normal brain specimens and meningioma from adult patients using reverse transcriptionpolymerase chain reaction (RT-PCR). This group of antigens was chosen because it includes many of the more traditionally investigated CTA transcripts.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cancer-testis and melanocyte-differentiation antigen expression in malignant glioma and meningioma

Syed¹,

Mandigo²,

Killory³

et al. 2012

Journal of Clinical Neuroscience

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cancer-testis and melanocyte-differentiation antigen expression in malignant glioma and meningioma

Syed¹,

Mandigo²,

Killory³

et al. 2012

Journal of Clinical Neuroscience

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“…Many of the proteins that constitute the synaptonemal complex are aberrantly expressed in cancers. For example, SCP1 expression has been described in brain, breast, renal cell, gastric, lung, stomach, and pancreatic carcinomas and melanoma (CT Gene Database) [ 57 , 58 , 59 , 60 , 61 , 62 , 63 , 107 ], and SCP3 is expressed in non-small cell lung cancers and leukemia [ 53 , 54 , 108 ].…”

Section: The Role Of Meiotic Proteins In Tumorigenesismentioning

confidence: 99%

Ectopic Expression of Testis Germ Cell Proteins in Cancer and Its Potential Role in Genomic Instability

Nielsen

Gjerstorff

2016

IJMS

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Genomic instability is a hallmark of human cancer and an enabling factor for the genetic alterations that drive cancer development. The processes involved in genomic instability resemble those of meiosis, where genetic material is interchanged between homologous chromosomes. In most types of human cancer, epigenetic changes, including hypomethylation of gene promoters, lead to the ectopic expression of a large number of proteins normally restricted to the germ cells of the testis. Due to the similarities between meiosis and genomic instability, it has been proposed that activation of meiotic programs may drive genomic instability in cancer cells. Some germ cell proteins with ectopic expression in cancer cells indeed seem to promote genomic instability, while others reduce polyploidy and maintain mitotic fidelity. Furthermore, oncogenic germ cell proteins may indirectly contribute to genomic instability through induction of replication stress, similar to classic oncogenes. Thus, current evidence suggests that testis germ cell proteins are implicated in cancer development by regulating genomic instability during tumorigenesis, and these proteins therefore represent promising targets for novel therapeutic strategies.

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“…MAGE-D4a is expressed in a large percentage of human CNS tumors, indicating that it may serve as a potential target for immunotherapy treatment (15,16). In the present study, ecto-mesenchymal stem cells (EMSCs) were isolated and cultured, and subsequently infected with an adenoviral plasmid containing MAGE-D4a (pAd/MAGE-D4a).…”

Section: Induction Of Antigen-specific Cytotoxic T-cell Response By Dmentioning

confidence: 99%

Induction of antigen-specific cytotoxic T-cell response by dendritic cells generated from ecto-mesenchymal stem cells infected with an adenovirus containing the MAGE-D4a gene

Shen

et al. 2016

Oncology Letters

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Abstract. The present study aimed to investigate the feasibility of using ecto-mesenchymal stem cell (EMSC)-derived dendritic cells (DCs) for glioma immunotherapy following infection by a recombinant adenovirus containing the melanoma-associated antigen D4a (MAGE-D4a) gene. The ex vivo cultured EMSCs were infected by the adenoviral plasmid containing MAGE-D4a (pAd/MAGE-D4a). Efficiency of transfection was evaluated through the detection of green fluorescent protein-marked MAGE-D4a. The MAGE-EMSCs were induced to differentiate into DCs, termed as MAGE-EMSCs-DCs. The morphology was subsequently analyzed under a microscope, and methyl thiazolyl tetrazolium (MTT) and interferon-γ (IFN-γ) assays were performed to analyze the cytotoxicity of the MAGE-EMSC-DCs on the human glioma U251 cell line. Following purification by magnetic-activated cell sorting, the EMSCs grew into swirls, with a long spindle shape and were fibroblast-like. The gene transfected with recombinant adenovirus vectors maintained high and stable expression levels of MAGE-D4a, and its efficiency was increased in a multiplicity of infection-dependent manner. The results of the MTT assay indicated that the T cells, primed by the recombinant MAGE-D4a-infected EMSC-DCs in vitro, recognized MAGE-D4a-expressing tumor cell lines in a human leukocyte antigen class I-restricted manner, and evoked a higher cytotoxic T cell (CTL) response. The CTL response induced by the MAGE-EMSC-DCs, co-cultured with the U251 cells for 24 h, produced 765.0 pg/ml IFN-γ, which was significantly greater when compared to the control wells. T lymphocytes stimulated by MAGE-EMSC-DCs evoke a higher CTL response to human glioma cell lines, and may serve as a promising therapeutic modality for the treatment of MAGE-D4a-expressing glioma.

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Expression of Cancer-Testis Genes in Brain Tumors

Cited by 16 publications

References 20 publications

Cancer-testis and melanocyte-differentiation antigen expression in malignant glioma and meningioma

Cancer-testis and melanocyte-differentiation antigen expression in malignant glioma and meningioma

Ectopic Expression of Testis Germ Cell Proteins in Cancer and Its Potential Role in Genomic Instability

Induction of antigen-specific cytotoxic T-cell response by dendritic cells generated from ecto-mesenchymal stem cells infected with an adenovirus containing the MAGE-D4a gene

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