Expression of cancer/testis (CT) antigens in lung cancer

Tajima, Kohei; Obata, Yuichi; Tamaki, Hiromi; Yoshida, Masahiro; Chen, Yao‐Tseng; Scanlan, Matthew J.; Old, Lloyd J.; Kuwano, Hiroyuki; Takahashi, Takashi; Takahashi, Toshitada; Mitsudomi, Tetsuya

doi:10.1016/s0169-5002(03)00244-7

Cited by 120 publications

(104 citation statements)

References 37 publications

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“…Although the number of cases of adenocarcinoma was very limited, the positive rates with using the SSX4 expression in lung cancer tissue were similar to those of a prior study (14). Thus, these molecular markers may be useful for lung cancer detection in clinical specimens.…”

Section: Discussionsupporting

confidence: 74%

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Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid

et al. 2007

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Section: Discussionsupporting

confidence: 74%

“…SSX has nine subtypes, SSX1 to SSX9 (11); among the different subtypes, SSX4 has been reported to show a high expression (35%) in lung cancer (14). The combined use of MAGE A1-6 and SSX4 might increase the rate of detecting cancer.…”

Section: Introductionmentioning

confidence: 99%

Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid

et al. 2007

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“…The choice of targeted tumour antigen is of importance in the conduct of clinical trials of tumour immunotherapy. MAGE-A4 is an attractive antigen because of its wide expression in a variety of tumours and its immunogenicity (Tajima et al, 2003;Yakirevich et al, 2003;Lin et al, 2004;Prasad et al, 2004;Li et al, 2005). Earlier studies reported the generation of MAGE-A4-specific CTL clones and definition of its epitope peptides (Zhang et al, 2002;Miyahara et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Among these, MAGE-A, MAGE-B and MAGE-C show expression patterns as CT antigens (Van der Bruggen et al, 1991;De Plaen et al, 1994;Chomez et al, 2001). The expression of MAGE-A4 antigen in tumour has been reported in various human malignancies, ovarian neoplasms (Yakirevich et al, 2003), mucosal melanomas of the head and neck (Prasad et al, 2004), oesophageal adenocarcinomas (Lin et al, 2004), colorectal cancer (Li et al, 2005) and lung cancer (Tajima et al, 2003). Therefore, MAGE-A4 is one of the attractive target molecules in tumour immunotherapy.…”

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confidence: 98%

Identification of novel helper epitopes of MAGE-A4 tumour antigen: useful tool for the propagation of Th1 cells

et al. 2009

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MAGE-A4 has been considered as an attractive cancer-testis (CT) antigen for tumour immunotherapy. It has been well accepted that T-helper type 1 (Th1) cell-dominant immunity is critical for the successful induction of antitumour immunity in a tumour-bearing host. The adoptive Th1 cell therapy has been shown to be an attractive strategy for inducing tumour eradication in mouse systems. However, Th1-cell therapy using human tumour-specific Th1 cells, which were expanded from peripheral blood mononuclear cells (PBMCs) in a clinically useful protocol, has never been performed. Here, we first identified MAGE-A4-derived promiscuous helper epitope, peptide (MAGE-A4 280 -299), bound to both HLA-DPB1*0501 and DRB1*1403. Using the peptide, we established a suitable protocol for the propagation of MAGE-A4-specific Th1 cells in vitro. Culture of CD4 þ T cells with IFN-g-treated PBMCderived adherent cells in the presence of helper epitope peptide resulted in a great expansion of MAGE-A4-reactive Th cells producing IFN-g , but not IL-4. Moreover, it was shown that ligation of MAGE-A4-reactive Th1 cells with the cognate peptide caused the production of IFN-g and IL-2. Thus, our identified MAGE-A4 helper epitope peptide will become a good tool for the propagation of tumour-specific Th1 cells applicable to adoptive immunotherapy of human cancer.

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“…It is reported that, about 10% of the genes on the X chromosome are CT antigen genes (4), and these CT-X genes are notably more testis-restrictively than those on other chromosomes (5). However, CT proteins expression shows heterogeneous in many cancers including lung carcinoma, therefore more CT antigens need to be identified to develop the polyvalent vaccines to avoid immune escape of tumors (1,6).…”

Section: Introductionmentioning

confidence: 99%

Promoter demethylation mediates the expression of ZNF645, a novel cancer/testis gene

Bai¹,

Zhang²,

Su³

et al. 2010

BMB Reports

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Cancer/testis (CT) antigens exhibit highly tissue-restricted expression and are considered promising targets for cancer vaccines. Here we identified a novel CT gene ZNF645 which restrictively expresses in normal human testes and lung cancer patients (68.3%). To investigate the promoter methylation status of ZNF645, we carried out bisulfite genomic sequencing and found that the CpG island in its promoter was heavily methylated in normal lung tissues without the expression of ZNF645, whereas there was high demethylation in normal human testes and lung carcinoma tissues with its expression. Also ZNF645 could be remarkably activated in A549 and HEK293T cells treated by DNA demethylation agent 5'-aza-2'-deoxycytidine. And the dual luciferase assay revealed that the promoter activity of the ZNF645 was inhibited by methylation of the CpG island region. Therefore, we proposed that ZNF645 is a CT gene and activated in human testis and lung cancers by demethylation of its promoter region. [BMB reports 2010; 43(6): 400-406]

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Expression of cancer/testis (CT) antigens in lung cancer

Cited by 120 publications

References 37 publications

Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid

Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid

Identification of novel helper epitopes of MAGE-A4 tumour antigen: useful tool for the propagation of Th1 cells

Promoter demethylation mediates the expression of ZNF645, a novel cancer/testis gene

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