Expression of Calcification and Extracellular Matrix Genes in the Cardiovascular System of the Healthy Domestic Sheep (Ovis aries)

Tsang, Hiu‐Gwen; Clark, Emily L.; Markby, Greg; Bush, Stephen J.; Hume, David; Corcoran, Brendan; MacRae, Vicky; Summers, Kim M.

doi:10.3389/fgene.2020.00919

Cited by 10 publications

(8 citation statements)

References 116 publications

(161 reference statements)

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“…We observed the increased expression of extracellular matrix (ECM) related proteins in AVE cells. A previous RNA expression study also showed the enrichment of ECM-related genes in the valve of domestic sheep ( Ovis aries ) [ 29 ]. The ECM provides structures necessary for the connection between cells and directs cellular functions in the cardiovascular system.…”

Section: Discussionmentioning

confidence: 99%

Proteomics Analysis Reveals Diverse Molecular Characteristics between Endocardial and Aortic-Valvular Endothelium

Kumar

Satheesh

et al. 2021

Genes

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The variations in the protein profile of aortic-valvular (AVE) and endocardial endothelial (EE) cells are currently unknown. The current study’s objective is to identify differentially expressed proteins and associated pathways in both the endothelial cells. We used endothelial cells isolated from the porcine (Sus scrofa) aortic valve and endocardium for the profiling of proteins. Label-free proteomics was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our proteomics analysis revealed that 29 proteins were highly expressed, and 25 proteins were less expressed in the valve than the endocardial endothelium. The cell surface markers, such as CD63, ICAM1, PECAM1, PROCR, and TFRC, were highly expressed in EE. In contrast, CD44 was highly expressed in AVE. The pathway analysis showed that metabolic process-related proteins and extracellular matrix-related proteins were enriched in valves. Differential enrichment of signaling pathways was observed in the endocardium. The hemostasis function-related proteins were increased in both endothelial cells. The proteins and pathways enriched in aortic-valvular and endocardial endothelial cells revealed the distinct phenotype of these two closely related cells.

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Section: Discussionmentioning

confidence: 99%

Proteomics Analysis Reveals Diverse Molecular Characteristics between Endocardial and Aortic-Valvular Endothelium

Kumar

Satheesh

et al. 2021

Genes

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“…More specifically, injection of recombinant human biglycan (rhBGN) has been proposed as a potential therapeutic intervention for DMD following promising studies in mdx mice 67 . In other diseases, upregulation of Bgn has been linked to contribute to a pro-osteogenic effect in vascular calcification 68,69 . Moreover, Bgn -deficient mice show reduced growth, decreased bone mass and osteoporosis 70 .…”

Section: Discussionmentioning

confidence: 99%

Spatial transcriptomics reveal markers of histopathological changes in Duchenne muscular dystrophy mouse models

Heezen

Abdelaal

Putten

et al. 2022

Preprint

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Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene, leading to lack of dystrophin. Chronic muscle damage eventually leads to histological alterations in skeletal muscles. The identification of genes and cell types driving tissue remodeling is a key step to develop effective therapies. Here we use spatial transcriptomics in two DMD mouse models differing in disease severity to identify gene expression signatures underlying skeletal muscle pathologies and directly link this to the muscle histology. Deconvolution analysis allowed the identification of cell types contributing to histological alterations. We show how the expression of specific genes is enriched in areas of muscle regeneration (Myl4, Sparc, Hspg2), fibrosis (Vim, Fn1, Thbs4) and calcification (Bgn, Ctsk, Spp1). Finally, our analysis of differentiation dynamics in the severely affected D2-mdx muscle shows a subset of the muscle fibers are predicted to become affected in its future state. Genes associated with tissue remodeling could enable to design new diagnostic and therapeutic strategies for DMD.

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“…Sheep may represent a promising animal alternative for obtaining VICs liable to calcify. Sheep exhibit an elevated calcium metabolism compared to humans, and this has led to their use as an experimental model of heart valve calcification [84]. Several authors have made use ovine VICs for experimentation [85,86].…”

Section: Sources Of Valvular Interstitial Cellsmentioning

confidence: 99%

Valvular Interstitial Cells: Physiology, Isolation, and Culture

Ground,

Callon,

Walker

et al. 2023

Technologies in Cell Culture - A Journey From Basics to Advanced Applications [Working Title]

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Valvular interstitial cells (VICs) are the primary cellular component of the heart valve. Their function is to maintain the structure of the valve leaflets as they endure some three billion beats in the course of a human lifespan. Valvular pathology is becoming ever more prevalent in our ageing world, and there has never been a greater need for understanding of the pathological processes that underpin these diseases. Despite this, our knowledge of VIC pathology is limited. The scientific enquiry of valve disease necessitates stable populations of VICs in the laboratory. Such populations are commonly isolated from porcine and human tissue. This is achieved by digesting valve tissue from healthy or diseased sources. Understanding of the many VIC phenotypes, and the biochemical cues that govern the transition between phenotypes is essential for experimental integrity. Here we present an overview of VIC physiology, and a tried-and-true method for their isolation and culture. We make mention of several biochemical cues that the researcher may use in their culture media to ensure high quality and stable VIC populations.

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Expression of Calcification and Extracellular Matrix Genes in the Cardiovascular System of the Healthy Domestic Sheep (Ovis aries)

Cited by 10 publications

References 116 publications

Proteomics Analysis Reveals Diverse Molecular Characteristics between Endocardial and Aortic-Valvular Endothelium

Proteomics Analysis Reveals Diverse Molecular Characteristics between Endocardial and Aortic-Valvular Endothelium

Spatial transcriptomics reveal markers of histopathological changes in Duchenne muscular dystrophy mouse models

Valvular Interstitial Cells: Physiology, Isolation, and Culture

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