Expression of BRCA1 and BRCA2 in different tumor cell lines with various growth status

Vissac, Cécile; Latour, Monique Peffault De; Communal, Y; Bignon, Yves‐Jean; Bernard‐Gallon, Dominique

doi:10.1016/s0009-8981(02)00055-4

Cited by 12 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, high levels of BRCA2 mRNA have been detected in androgen‐independent prostate cancer cell lines 28. Since expression of BRCA genes appeared to be upregulated after cell proliferation in human tumour cell lines and is believed to exert a growth‐inhibitory action,28, 29 overexpression of BRCA2 in advanced androgen‐independent prostate cancer may represent a feedback mechanism and could reflect, like Top2A and Ki67 , the high proliferation rate of HRPC. Hepsin is a transmembrane serine protease that is overexpressed in several cancers, including renal cell carcinoma and prostate cancer 30, 31.…”

Section: Discussionmentioning

confidence: 97%

Differential expression of 37 selected genes in hormone‐refractory prostate cancer using quantitative taqman real‐time RT‐PCR

Fromont

Chêne

Vidaud³

et al. 2004

Intl Journal of Cancer

View full text Add to dashboard Cite

Progression of prostate cancer to androgen independence remains the primary obstacle to improved survival. The development of more effective treatments depends on our understanding of the molecular events associated with the hormone-refractory stage. We quantified, among 90 screened genes, the expression of 37 target genes, using real-time quantitative RT-PCR. Gene expression was studied in 13 samples of HPRC compared to 33 Prostate cancer is one of the most common cancers in men and the second leading cause of cancer-related death among men in industrialised countries. 1 Androgens stimulate the growth of both normal prostate and prostate cancer, and disease progression is characterised by the transition from an androgen-dependent to an androgen-independent phenotype. Although 80% of patients with advanced prostate cancer first respond to androgen ablation therapy, within 12-18 months the tumour eventually progresses to an androgen-independent stage, resulting in poor prognosis. 2 The complex mechanisms underlying the evolution to androgen independence remain poorly understood. However, there is increasing evidence that the AR signalling pathway is associated with progression to the hormone-refractory stage, via either amplification or mutation of the AR gene. 3 Other molecular mechanisms that could be related to the multiple genetic alterations observed in advanced prostate cancer are also likely to be involved in the transition towards an androgen-independent stage, including changes in growth and apoptotic factors and neuroendocrine differentiation. Because there is no effective treatment for the hormone-refractory stage of advanced prostate cancer, understanding the mechanisms associated with the progression to androgen independence is critical to the development of new therapeutic strategies. Few studies are, however, available concerning the expression profile of advanced HRPC, probably in part because samples from hormone-resistant tumours are very rare. In the present study, we analysed by real-time quantitative RT-PCR the expression of genes involved in pathways (e.g., steroid metabolism, cell cycling, apoptosis, signal transduction, DNA repair) that are known to be dysregulated in solid tumours, including prostate carcinoma, to identify differential gene expression between normal prostate tissue, clinically localised and hormone-refractory prostate cancer. Material and methods Patients and tissuesClinically localised prostate tumours were obtained from 33 patients undergoing radical prostatectomy with lymph node excision and negative nodal status, including 20 cancers limited to the prostate (pT2) and 13 with extracapsular extension (pT3). Staging was assessed after pathologic examination of formalin-fixed specimens on the basis of the 1997 TNM classification. HRPC samples were obtained from 13 patients after transurethral resection. Clinical and biologic data from the 46 patients are provided (Table I). Prostatic tissue samples from 16 patients were used to assess basal levels of mRNA from target gen...

show abstract

Section: Discussionmentioning

confidence: 97%

Differential expression of 37 selected genes in hormone‐refractory prostate cancer using quantitative taqman real‐time RT‐PCR

Fromont

Chêne

Vidaud³

et al. 2004

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…In estrogen-positive receptor breast cancer cells (the characteristic of MCF7 cells), cytosolic BRCA1 expression is inversely related to survival (68). Furthermore, transcriptional activity of BRCA1 and BRCA2 genes has been observed in multiple breast cancers (including MCF-7 cells) (69)(70)(71). In our study, we found that lactate exposure is a potent regulator of their transcriptional activity with increases in mRNA expression between 3.3-and 6.1-fold (p < 0.001) ( Table 1, Figures 2, 3A,B).…”

Section: Discussionmentioning

confidence: 99%

Is Lactate an Oncometabolite? Evidence Supporting a Role for Lactate in the Regulation of Transcriptional Activity of Cancer-Related Genes in MCF7 Breast Cancer Cells

et al. 2020

View full text Add to dashboard Cite

Lactate is a ubiquitous molecule in cancer. In this exploratory study, our aim was to test the hypothesis that lactate could function as an oncometabolite by evaluating whether lactate exposure modifies the expression of oncogenes, or genes encoding transcription factors, cell division, and cell proliferation in MCF7 cells, a human breast cancer cell line. Gene transcription was compared between MCF7 cells incubated in (a) glucose/glutamine-free media (control), (b) glucose-containing media to stimulate endogenous lactate production (replicating some of the original Warburg studies), and (c) glucose-containing media supplemented with L-lactate (10 and 20 mM). We found that both endogenous, glucose-derived lactate and exogenous, lactate supplementation significantly affected the transcription of key oncogenes (MYC, RAS, and PI3KCA), transcription factors (HIF1A and E2F1), tumor suppressors (BRCA1, BRCA2) as well as cell cycle and proliferation genes involved in breast cancer (AKT1, ATM, CCND1, CDK4, CDKN1A, CDK2B) (0.001 < p < 0.05 for all genes). Our findings support the hypothesis that lactate acts as an oncometabolite in MCF7 cells. Further research is necessary on other cell lines and biopsy cultures to show generality of the findings and reveal the mechanisms by which dysregulated lactate metabolism could act as an oncometabolite in carcinogenesis.

show abstract

“…In the present study, the expression of BRCA1, BRCA2, IGF-I and AR mRNAs was measured with quantitative realtime PCR (TaqMan ® System) (31,35). The probes and the primers used for the quantification were chosen with the help of a primer express computer program, the results of which are summarized in Table I.…”

Section: Methodsmentioning

confidence: 99%

BRCA1, BRCA2, AR and IGF-I expression in prostate cancer: Correlation between RT-qPCR and immunohistochemical detection

Rabiau

Déchelotte²,

Adjakly³

et al. 2011

Oncol Rep

Self Cite

View full text Add to dashboard Cite

Abstract. Identification and characterization of biomarkers in prostate cancer are important for improving the diagnosis. The aim of this study was to determine differences in the expression of 4 genes according to the stage of malignancy in prostate cancer. We analyzed BRCA1, BRCA2, androgen receptor (AR) and IGF-I gene expression in a cohort of 98 prostate biopsies. We used TaqMan RT-qPCR for mRNA detection, and correlation with proteins was performed using immunohistochemistry. Among the 98 studied prostate biopsies, high heterogeneity in the expression of the 4 genes was detected among the different histological types. However, down-regulation of BRCA1 and BRCA2 mRNA was detected, particularly in the normal tissues. The expression of AR was dependent on the stage of the tumor. The IGF-I gene was specifically expressed in the tumor tissues. Upon comparison between protein and mRNA expression for BRCA1, BRCA2 and AR, we obtained a trend; however, this did not achieve statistical significance. Regarding IGF-I, a correlation between mRNA expression and staining intensity of the protein was found to be significant (p<0.012). The AR biomarker was found to be slightly correlated with the prostate cancer diagnosis (p=0.013). AR was found to be decreased in the tumors with a 43% sensitivity and 90% specificity. The relative risk of 2.05 (1.13-3.69) indicated a 2-fold higher chance of cancer occurence when AR was ≤0.206.

show abstract

Expression of BRCA1 and BRCA2 in different tumor cell lines with various growth status

Cited by 12 publications

References 22 publications

Differential expression of 37 selected genes in hormone‐refractory prostate cancer using quantitative taqman real‐time RT‐PCR

Differential expression of 37 selected genes in hormone‐refractory prostate cancer using quantitative taqman real‐time RT‐PCR

Is Lactate an Oncometabolite? Evidence Supporting a Role for Lactate in the Regulation of Transcriptional Activity of Cancer-Related Genes in MCF7 Breast Cancer Cells

BRCA1, BRCA2, AR and IGF-I expression in prostate cancer: Correlation between RT-qPCR and immunohistochemical detection

Contact Info

Product

Resources

About