Expression of bioactive porcine interferon-alpha in Lactobacillus casei

Ma, Shi-Jie; Li, Kun; Li, Xinsheng; Guo, Xingchen; Fu, Pengfei; Yang, Mingfan; Chen, Hongying

doi:10.1007/s11274-014-1663-7

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…But there were only a few genetic tools developed in other LAB except L. lactis which were used in probiotic products. Secondly, most expression system developed in LAB were using inducible promoters, such as P nisA , P zn , P lac , P 170 which were induced by nisin, heavy metals, sugar and pH, respectively [29][30][31][32]. Inducible expression can be preferable in cases where the aim is to overproduce a desired protein at high levels, e.g.…”

Section: Resultsmentioning

confidence: 99%

Development of A Novel Expression System In Lactic Acid Bacteria Controlled By A Broad-Host-Range Promoter PsrfA

Guan

Yuan

et al. 2021

Preprint

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Background: Latic acid bacteria (LAB) are exploited for development of gene expression system owing to its health promoting properties and a high degree of safety status. Most of the expression systems were constructed in Lactobacillus lactis with inducible promoters. It is necessary to exploit novel promoters to develop LAB host platforms which are indispensable in dairy and health application to satisfy the production demand of increased number of target-genes. Previously, promoter PsrfA had been displayed broad host range and used to construct auto-inducible expression system in B. subtilis and E. coli. In this work, the feasibility of PsrfA in lactic acid bacteria (LAB) was estimated.Results: Plasmid with the green fluorescent protein (GFP) inserting downstream of PsrfA was transformed into L. casei 5257, L. plantarum 97, L. fermentum 087 and Weissella confusa 10, respectively. The recombinant strains grew well and displayed different fluorescence which could be detected by spectrophotometer and laser scanning confocal microscope. Moreover, the promoter activity was strain- specifically influenced by particular carbon and nitrogen sources. Heterologous laccase CotA could be expressed by PsrfA in L. casei 5257-05 and L. plantarum 97-06. By adjusting the pH value from 4.5 to 6.5 during incubation, the CotA activity of L. plantarum 97-05 and L. casei 5257-05 was increased by 137.7 % and 61.5 %, respectively. Finally, the fermentation pH was variably up-regulated along with the production of NADH oxidase controlled by the PsrfA and its derivative mutated with core regions. Conclusions: These data suggested that PsrfA was valid for gene expression in different species of LAB. Moreover, PsrfA could be used as an attractive candidate for fine-tuning gene expression in a broad range of procaryotic expression plants.

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Section: Resultsmentioning

confidence: 99%

Development of A Novel Expression System In Lactic Acid Bacteria Controlled By A Broad-Host-Range Promoter PsrfA

Guan

Yuan

et al. 2021

Preprint

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“…The porcine IFN-λ3 can be displayed on the surface of the membrane via a transmembrane helix anchor (the pgsA protein from Bacillus subtilis ) or attached to the cell wall anchor via a covalent link with the LPXTG motif (CWA) [ 38 ]. Hence, the use of CWA and pgsA to display porcine IFN-λ3 on the surface of L. plantarum is a promising candidate approach for the use of L. plantarum as a live oral bacterial agent [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Surface-Displayed Porcine IFN-λ3 in Lactobacillus plantarum Inhibits Porcine Enteric Coronavirus Infection of Porcine Intestinal Epithelial Cells

Liu

Jiang

et al. 2020

Journal of Microbiology and Biotechnology

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Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity.Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

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“…In this study, pSIP expression systems were used to express FMDV-VP1 protein. Studies are currently underway to employ lactic acid bacteria vectors to produce vaccines of many viral proteins, such as the hemagglutinin (HA) gene of H9N2 avian influenza virus, anti-rotavirus protein (Llama VHH antibody fragments), and bioactive porcine interferon-alpha [48][49][50]. Two L. plantarum strains were chosen in this study to carry the pSIP plasmids.…”

Section: Discussionmentioning

confidence: 99%

Protection against Foot-and-Mouth Disease Virus in Guinea Pigs via Oral Administration of Recombinant Lactobacillus plantarum Expressing VP1

et al. 2015

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Mucosal vaccination is an effective strategy for generating antigen-specific immune responses against mucosal infections of foot-and-mouth disease virus (FMDV). In this study, Lactobacillus plantarum strains NC8 and WCFS1 were used as oral delivery vehicles containing a pSIP411-VP1 recombinant plasmid to initiate mucosal and systemic immune responses in guinea pigs. Guinea pigs were orally vaccinated (three doses) with NC8-pSIP411, NC8-pSIP411-VP1, WCFS1-pSIP411, WCFS1-pSIP411-VP1 or milk. Animals immunized with NC8-pSIP411-VP1 and WCFS1-pSIP411-VP1 developed high levels of antigen-specific serum IgG, IgA, IgM, mucosal secretory IgA (sIgA) and neutralizing antibodies, and revealed stronger cell-mediated immune responses and enhanced protection against FMDV challenge compared with control groups. The recombinant pSIP411-VP1 effectively improved immunoprotection against FMDV in guinea pigs.

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Expression of bioactive porcine interferon-alpha in Lactobacillus casei

Cited by 8 publications

References 26 publications

Development of A Novel Expression System In Lactic Acid Bacteria Controlled By A Broad-Host-Range Promoter PsrfA

Development of A Novel Expression System In Lactic Acid Bacteria Controlled By A Broad-Host-Range Promoter PsrfA

Surface-Displayed Porcine IFN-λ3 in Lactobacillus plantarum Inhibits Porcine Enteric Coronavirus Infection of Porcine Intestinal Epithelial Cells

Protection against Foot-and-Mouth Disease Virus in Guinea Pigs via Oral Administration of Recombinant Lactobacillus plantarum Expressing VP1

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