Expression of Bax and Apoptosis-Related Proteins in Human Esophageal Squamous Cell Carcinoma Including Dysplasia

Kurabayashi, Atsushi; Furihata, Mutsuo; Matsumoto, Manabu; Ohtsuki, Yuji; Sasaguri, Shiro; Ogoshi, Shohei

doi:10.1038/modpathol.3880383

Cited by 35 publications

(32 citation statements)

References 26 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In agreement with previous studies [21], the apoptotic cells that displayed apoptotic morphology (cell shrinkage and chromatin condensation and/or nuclear fragmentation) were counted in areas with high frequency under 10 high-power microscopic fields. More than 1,000 tumor cells were counted to calculate the apoptotic index (AI) for these areas, and the AI values were expressed as percentages of TUNEL-positive cells.…”

Section: Methodssupporting

confidence: 60%

Prognostic Significance of Serine 392 Phosphorylation in Overexpressed p53 Protein in Human Esophageal Squamous Cell Carcinoma

Matsumoto

Furihata²,

Kurabayashi³

et al. 2004

Oncology

Self Cite

View full text Add to dashboard Cite

Objective: Posttranscriptional modification involving phosphorylation of wild-type p53 has been considered to play a role in the stabilization of p53. Little is known, however, about the role of phosphorylation of mutant p53 overexpressed in tumors. The aim of this study is to determine the phosphorylation state of p53 in tumors and its contribution to tumor development. Methods: Using immunohistochemical techniques, we examined the phosphorylation of Ser392 and 15 sites in p53 overexpressed in 137 esophageal squamous cell carcinomas (ESCCs). The relationships between the phosphorylation and the expression of cell cycle regulators, Ki-67 labeling index (LI), apoptotic index, clinicopathological factors, and patient prognosis were tested statistically. Results: Of the 137 samples examined, staining for Ser392 phosphospecific p53 antibody was detected in 53 (38.7%) cases, corresponding to 58.9% of 90 p53-positive cases, whereas only 3 (2.2%) were positive for Ser15 phosphospecific p53 antibody. A significant correlation was identified between Ser392 phosphorylation and high levels of Ki-67 LI (p = 0.0271), lymphatic invasion (p = 0.0246), and poorer prognosis for patients with stage II and III advanced tumors (p = 0.0136). Using multivariate analysis, Ser392 phosphorylation was recognized as an independent prognostic factor (p = 0.0136). Conclusions: These findings suggest that p53 protein overexpressed in ESCCs is frequently phosphorylated at Ser392, and that the Ser392 phosphorylation might contribute to tumor progression of ESCCs.

show abstract

Section: Methodssupporting

confidence: 60%

Prognostic Significance of Serine 392 Phosphorylation in Overexpressed p53 Protein in Human Esophageal Squamous Cell Carcinoma

Matsumoto

Furihata²,

Kurabayashi³

et al. 2004

Oncology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The results of the p53 immunoexpression in this investigation did not associate with the postoperative survival (P = 0,303) and, also did not present any association with the clinicopathological parameters by univariate analysis. These findings are in accordance with other published data (5,6,7,17,18,21,23,25,31,36,39) . IRELAND et al (17) verified p53 mutations in 48.6% of the studied adenocarcinomas, being 53% of esophagus and 58% of the cardia.…”

Section: Discussionsupporting

confidence: 83%

Adenocarcinoma of the esophagogastric junction: relationship between clinicopathological data and p53, cyclin D1 and Bcl-2 immunoexpressions

Lehrbach

Cecconello

Ribeiro

et al. 2009

Arq. Gastroenterol.

View full text Add to dashboard Cite

-Context -Esophagogastric junction adenocarcinoma has an aggressive behavior, and TNM (UICC) staging is not always accurate enough to categorize patient's outcome. Objectives -To evaluated p53, cyclin D1 and Bcl-2 immunoexpressions in esophagogastric junction adenocarcinoma patients, without Barrett's esophagus, and to compared to clinicopathological characteristics and survival rate. Methods -Tissue sections from 75 esophagogastric junction adenocarcinomas resected from 1991 to 2003 were analyzed by immunohistochemistry for p53, cyclin D1 and Bcl-2 using streptavidin-biotin-peroxidase method. The mean follow-up time was 60 months SD = 61.5 (varying from 4 to 273 months). Results -Fifty (66.7%) of the tumors were intestinal type and 25 (33.3%) were diffuse. Vascular, lymph node and perineural infiltration were verified in 16%, 80% and 68% of the patients, respectively. The patients were distributed according to the TNM staging in IA in 4 (5.3%), IB in 10 (13.3%), II in 15 (20%), IIA in 15 (20%), IIIB in 15 (20%) and IV in 16 (21.3%). Immunohistochemical analysis was positive for p53, cyclin D1 and bcl-2 in 68%, 18.7% and 100%, respectively. There was no association between immunoexpression and vascular and/or perineural invasions, clinicopathological characteristics and patients' survival rate. Conclusion -In this selected population, there was no association between the immunomarkers, p53, cyclin D1 and bcl-2 and clinicopathological data and/or overall survival. HEADINGS -Esophagogastric junction. Stomach neoplasms. Esophageal neoplasms. Adenocarcinoma. Tumor suppressor protein p53.Cyclin D1. Proto-oncogene proteins c-bcl-2.

show abstract

“…The cut‐off point of 3% for the distinction between high and low apoptotic potential was selected close to the mean and median AI, resulting in a statistically significant correlation between the AI and the percentage of bax‐positive tumour cells (Table 2). Similar correlations with the AI were found in squamous head and neck carcinoma (37), breast carcinoma (38), as well as in dysplastic esophageal epithelium (39). In some solid tumours, however, no correlation could be found between the Bax expression and the apoptotic activity, suggesting a loss of function of this protein (39, 40).…”

Section: Discussionsupporting

confidence: 69%

Bax, Bcl‐2, Fas and Fas‐L antigen expression in human seminoma: Correlation with the apoptotic index

Grobholz¹,

Zentgraf²,

Köhrmann³

et al. 2002

APMIS

View full text Add to dashboard Cite

Apoptosis plays a crucial role in the regulation of spermatogenesis in male germ cells and is, at least in part, modulated by Bcl-2, Bax, and the Fas pathway. Seminomas have a favourable outcome and respond to radio-/chemotherapy with an increased rate of apoptosis. The expression of Bax, Bcl-2, Fas and Fas-ligand (Fas-L) in human seminoma was evaluated and correlated with the apoptotic index. Twenty-nine classical seminomas were examined by immunohistochemistry and Western blotting using antibodies against Bax, Bcl-2, Fas and Fas-L. Apoptosis was detected by in-situ end-labeling of fragmented DNA and the apoptotic index (AI) was determined. Expression of Fas was found in 26 (89.7%) of Fas-L in 24 seminomas (82.2%); none of the tumours expressed Bcl-2. No correlation between the AI and Fas, Fas-L or Bcl-2 expression was found. Bax was demonstrated in 20/29 tumours (69%). Bax-positive tumours showed an increased AI of 4.75 +/- 2.38% in contrast to 2.60 +/- 1.23% of the Bax-negative tumours (P = 0.002). The number of Bax-positive tumour cells and apoptotic cells revealed a significant correlation using chi2-test (P = 0.04) and linear regression (r = 0.54, P = 0.001). Therefore, Bax seems to play a determinant role in the modulation of apoptosis in human seminoma that may be linked to a favourable outcome.

show abstract

Expression of Bax and Apoptosis-Related Proteins in Human Esophageal Squamous Cell Carcinoma Including Dysplasia

Cited by 35 publications

References 26 publications

Prognostic Significance of Serine 392 Phosphorylation in Overexpressed p53 Protein in Human Esophageal Squamous Cell Carcinoma

Prognostic Significance of Serine 392 Phosphorylation in Overexpressed p53 Protein in Human Esophageal Squamous Cell Carcinoma

Adenocarcinoma of the esophagogastric junction: relationship between clinicopathological data and p53, cyclin D1 and Bcl-2 immunoexpressions

Bax, Bcl‐2, Fas and Fas‐L antigen expression in human seminoma: Correlation with the apoptotic index

Contact Info

Product

Resources

About