Expression of Bacillar Glutamyl Endopeptidase Genes in Bacillus subtilis by a New Mobilizable Single-Replicon Vector pLF

Шевелев, А. Б.; Aleoshin, Vladimir V.; Trachuk, Lesya A.; Granovsky, Alexei E.; Kogan, Yakov; Rumer, Leonid; Serkina, A. V.; Семенова, Е. В.; Bushueva, Anastassia M.; Livshits, Vitaly A.; Kostrov, Sergey V.; Shcheglov, Alex S.; Novikova, Svetlana I.; Chestukhina, G. G.

doi:10.1006/plas.1999.1455

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Subtilisin 72, purified in this laboratory, was distributed over macroporous silica–silochrom as described earlier (12). Proteinases from B. intermedius and B. licheniformis were isolated in this laboratory (20).…”

Section: Methodsmentioning

confidence: 99%

Enzymatic synthesis of chromogenic substrates for Glu,Asp‐specific proteinases

Milgotina

Shcheglov

Lapa

et al. 2001

The Journal of Peptide Research

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Glu,Asp-specific endopeptidases represent a new subfamily of chymotrypsin-like proteolytic enzymes. These enzymes prefer Glu or Asp residues in the P1 position of the substrates. p-Nitroanilides of N-acylated di-, tri- and tetrapeptides with C-terminal glutamic or aspartic acid residues have been obtained. Acyl peptide p-nitroanilides were synthesized via acylation of glutamic or aspartic acid p-nitroanilides using methyl esters of the respective N-acylated peptides, generally with good yields. The reactions were performed in organic solvents using subtilisin 72 sorbed on silica as a catalyst. The kinetic parameters for the hydrolysis of these p-nitroanilides with proteinases from Bacillus intermedius and Bacillus licheniformis were determined.

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Section: Methodsmentioning

confidence: 99%

Enzymatic synthesis of chromogenic substrates for Glu,Asp‐specific proteinases

Milgotina

Shcheglov

Lapa

et al. 2001

The Journal of Peptide Research

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Salt stress induction of glutamyl endopeptidase biosynthesis in Bacillus intermedius

Gabdrakhmanova

Вишняков

Шарипова

et al. 2005

Microbiological Research

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Bacteria from the genus Bacillus have evolved complicated regulatory networks to be protected from various environmental stresses, including sudden increase in salinity. Among these regulatory mechanisms is the DegS-DegU signal transduction system, which controls degradative enzyme synthesis and is involved in sensing salt stress in Bacillus subtilis. We report the study of biosynthesis regulation of Bacillus intermedius glutamyl endopeptidase under salt stress conditions. Salt stress during growth in medium containing 1-2.5 M NaCl, KCl or disodium succinate leads to the induction of glutamyl endopeptidase. Analysis of the regulatory region of the gene for B. intermedius glutamyl endopeptidase revealed the presence of a tentative target sequence for DegU control, AGATN10TTGAG. For the expression of the glutamyl endopeptidase gene, functional DegU protein is required. Thus, we suggest that expression of the gene for B. intermedius glutamyl endopeptidase may be controlled by a regulatory system analogous to DegS-DegU two-component system in B. subtilis.

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