Expression of B-cell translocation gene 2 is associated with favorable prognosis in hepatocellular carcinoma patients and sensitizes irradiation-induced hepatocellular carcinoma cell apoptosis in vitro and in nude mice

Abstract

Abstract. B-cell translocation gene 2 (BTG2) proteins have been reported to be putative tumor suppressors in various cancer types. The present study first assessed BTG2 expression in 44 human liver cancer tissue specimens, then investigated BTG2 expression in the regulation of hepatocellular carcinoma (HCC) cell apoptosis with or without radiotherapy in vitro and in vivo. The results revealed that BTG2 protein expression was significantly reduced in HCC tissues, and associated with better survival for HCC patients (P=0.05). BTG2 overexpression also sensitized Huh7 cells to radiation-induced apoptosis in vitro and in a nude mouse model, although restoration of BTG2 expression per se did not affect the viability and apoptosis of HCC cells. Future studies would confirm the role of BTG2 in hepatoma, and further develop BTG2 as a therapeutic strategy for controlling HCC.

Exosomal and non-exosomal miRNA expression levels in patients with HCV-related cirrhosis and liver cancer

“…The third condition was identification of the miRNAs by other research groups in the biological fluids and cases of interest for us. Thus, according to MirAnalyze database, let-7a-5p, miRNA-16-5p, -21-5p share common target genes as follows: B-cell translocation gene 2 (BTG 2), and fibroblast growth factor receptor substrate 2 (FGFR2), which change their expression in cancer [18][19][20]. MiRNA-21-5p was one of the most significantly changed in cancer, what may allow to make a rough assumption that the expression level of its target genes should be decreased.…”

Screening of Long Noncoding RNAs Induced by Radiation Using Microarray

“…27 Series of other mRNAs such as SERHL2, PSRC1, BTG2, ATF3, and ARHGAP29 are also considered to be related to radiation. 22,23,28 -30 Meanwhile, many mRNAs such as KIAA1751, ERN1, and PIF1 are identified to be related to radiation for the first time in our profile. These findings provide new insights into relationship between radiation and cell survival.…”

“…It is well known that the G2/M checkpoint responds to radiation-induced DNA damage. 22,23 In order to detect G2/M arrest after radiation, we performed a flow cytometric analysis of HeLa, MCF-7, and A549 cells treated with radiation. Two hours after radiation at a dose of 4 Gy, cells were marked with the p-H3 antibody and PI and analyzed with flow cytometer.…”

The functional role of microRNAs in laryngeal carcinoma

“…Ren et al found that the migration ability of Hep-2 cells significantly decreased after inhibiting the expression of miR-21 by transfecting antisense oligonucleotides (ASO), while ASO-miR-21 could inhibit the expression of MMP-2 and Ras protein, leading to cell cycle arrest [14]. B cell translocation gene 2 (BTG2) is a kind of cell cycle regulator and tumor suppressor that can inhibit the proliferation of tumor cells [15]. The abnormal expression of miR-21 was able to promote the cancer process by downregulating the expression of BTG2 in laryngeal squamous cell carcinoma.…”