Expression of autophagy and apoptosis biomarkers in patients with acute ischemic stroke

Abstract

Introduction and Aim: Apoptosis, autophagy, and necrosis are the main mechanisms of neuron death in acute ischemic stroke (AIS). This study aimed to evaluate the expression of apoptosis and autophagy biomarkers in peripheral blood of patients with AIS. Materials and Methods: Sixty-eight patients (32 men and 36 women) aged 30-60 years with AIS underwent a clinical and neurological examination on the 1st, 7th, and 14th days after the disease onset. The expression of apoptosis and autophagy biomarkers in peripheral blood was evaluated by flow cytometry and compared with the severity of neurological deficit and injury on the 1st, 7th, and 14th days, using correlation analysis. Results: There is a statistical significance compared with the control group and an increase in the expression of key biomarkers of apoptosis and autophagy was revealed. Increased expression levels of annexin A5 and caspase-3 positively correlate with the severity of neurological deficit and injury on the 1st and 7th days from the onset of the disease. Conclusion: A direct correlation was revealed between elevated levels of apoptosis and autophagy biomarkers in peripheral blood and severity of neurological deficit and injury on the 1st, 7th, and 14th days from the onset of AIS.

A rare case of central post-stroke pain in a patient with migraine and lacunar infarcts in the medulla oblongata

“…In turn, acute ischemic stroke (AIS), is characterized by the sudden appearance of a neurological deficit of vascular origin, usually occurring after the age of 50 years (2,3). AIS is a type of stroke that remains a concern in neurology due to its wide prevalence, high disability, and mortality rates (4)(5)(6)(7).…”