Expression of asparagine synthetase genes in sunflower (Helianthus annuus) under various environmental stresses

Herrera-Rodríguez, María Begoña; Pérez‐Vicente, Rafael; Maldonado, José-María

doi:10.1016/j.plaphy.2006.12.002

Cited by 49 publications

(25 citation statements)

References 25 publications

(40 reference statements)

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“…The C/N ratio is one of the factors known to regulate asparagine synthetase levels (Herrera-Rodríguez et al, 2007). Thus, greater availability of N would stimulate its expression.…”

Section: Amino Acids Contentmentioning

confidence: 99%

Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea

Cruchaga

Artola

Lasa

et al. 2011

Journal of Plant Physiology

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The application of urease inhibitors in conjunction with urea fertilizers as a means of reducing N loss due to ammonia volatilization requires an in-depth study of the physiological effects of these inhibitors on plants. The aim of this study was to determine how the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) affects N metabolism in pea and spinach. Plants were cultivated in pure hydroponic culture with urea as the sole N source. After 2 weeks of growth for pea, and 3 weeks for spinach, half of the plants received NBPT in their nutrient solution. Urease activity, urea and ammonium content, free amino acid composition and soluble protein were determined in leaves and roots at days 0, 1, 2, 4, 7 and 9, and the NBPT content in these tissues was determined 48h after inhibitor application. The results suggest that the effects of NBPT on spinach and pea urease activity differ, with pea being most affected by this treatment, and that the NBPT absorbed by the plant caused a clear inhibition of the urease activity in pea leaf and roots. The high urea concentration observed in leaves was associated with the development of necrotic leaf margins, and was further evidence of NBPT inhibition in these plants. A decrease in the ammonium content in roots, where N assimilation mainly takes place, was also observed. Consequently, total amino acid contents were drastically reduced upon NBPT treatment, indicating a strong alteration of the N metabolism. Furthermore, the amino acid profile showed that amidic amino acids were major components of the reduced pool of amino acids. In contrast, NBPT was absorbed to a much lesser degree by spinach plants than pea plants (35% less) and did not produce a clear inhibition of urease activity in this species.

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“…The C/N ratio is one of the factors known to regulate asparagine synthetase levels (Herrera-Rodríguez et al, 2007). Thus, greater availability of N would stimulate its expression.…”

Section: Amino Acids Contentmentioning

confidence: 99%

Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea

Cruchaga

Artola

Lasa

et al. 2011

Journal of Plant Physiology

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“…N remobilization towards new developing organs is largely dependent on senescence-related catabolism events and translocation of leaf N compounds. Amino acids derived from protein catabolism are exported via the phloem to growing parts of the plant; the concentration of amino acids in the phloem sap increases during leaf senescence (Herrera-Rodriguez et al , 2006, 2007; Masclaux-Daubresse et al , 2006). In many species including B. napus , aspartate, glutamate, and their corresponding amides are the principal forms of amino N compounds transported in the phloem and play a key role in rendering N available for remobilization from senescing leaves (Tilsner et al , 2005).…”

Section: Introductionmentioning

confidence: 99%

Sixteen cytosolic glutamine synthetase genes identified in the Brassica napus L. genome are differentially regulated depending on nitrogen regimes and leaf senescence

Orsel¹,

Moison²,

Clouet³

et al. 2014

Journal of Experimental Botany

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“…These data suggest that, under B deprivation, AS might act as a complement to the GS-GOGAT cycle using ammonium to prevent this cation from reaching toxic concentrations in tobacco roots (Sivasankar and Oaks 1996). Furthermore, it has been reported that GS gene expression is affected by salinity, drought, and N starvation (Teixeira and Pereira 2007;Caputo et al 2009), and that an increase in AS transcript levels occurs with other abiotic stresses such as salinity, osmotic stress, heavy metal exposure and cold (Chevalier et al 1996;Wong et al 2004;Wang et al 2005;Herrera-Rodríguez et al 2007). …”

Section: Discussionmentioning

confidence: 99%

Boron deficiency increases expressions of asparagine synthetase, glutamate dehydrogenase and glutamine synthetase genes in tobacco roots irrespective of the nitrogen source

Beato

Rexach

Navarro-Gochicoa

et al. 2014

Soil Science and Plant Nutrition

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Nitrate-ammonium (N-A) and ammonium-nitrate (A-N) transition experiments were performed with tobacco (Nicotiana tabacum, cv Gatersleben) plants to study whether the increased expression of ammonium assimilation-related genes found under short-term boron (B) deficiency is maintained when the nitrogen source changes. Asparagine synthetase (AS), glutamate dehydrogenase (GDH) and glutamine synthetase (GS) gene overexpressions in roots under B deprivation were detected in both nitrogen transitions, although GS and, especially, AS up-regulations were observed earlier in the ammonium-nitrate transition. Transcript levels were inversely correlated with hexose contents after 24 h of B deprivation. Our results suggest that tobacco roots respond to short-term B deficiency by increasing the expression of AS, GDH and GS genes irrespectively of the nitrogen source. A hypothetical model to explain these results is proposed and discussed. Thus, the combined action of these enzymes would avoid the accumulation of ammonia and help maintain the activity of the tricarboxylic acid cycle when tobacco roots are affected by B deficiency, the plant organ that first senses this stress. Therefore, under B deficiency, they would act as an ammonium detoxifying mechanism, being a complement to the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway. We propose that the protective role of these enzymes could be extended to changing nitrogen-supply conditions.

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Expression of asparagine synthetase genes in sunflower (Helianthus annuus) under various environmental stresses

Cited by 49 publications

References 25 publications

Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea

Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea

Sixteen cytosolic glutamine synthetase genes identified in the Brassica napus L. genome are differentially regulated depending on nitrogen regimes and leaf senescence

Boron deficiency increases expressions of asparagine synthetase, glutamate dehydrogenase and glutamine synthetase genes in tobacco roots irrespective of the nitrogen source

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