Expression of artemisinin biosynthesis genes in eight Artemisia species at three developmental stages

Ranjbar, Mahdy; Naghavi, Mohammad Reza; Alizadeh, H; Soltanloo, Hassan

doi:10.1016/j.indcrop.2015.07.077

Cited by 29 publications

(20 citation statements)

References 56 publications

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“…absinthium had a higher expression level of both Aa - ALDH1 and Aa - CYP71AV1 genes when compared to A . annua during the developmental stages 5 . Salehi et al .…”

Section: Introductionmentioning

confidence: 99%

Expression of key genes affecting artemisinin content in five Artemisia species

Salehi

Karimzadeh

Naghavi

et al. 2018

Sci Rep

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Artemisinin, an effective anti-malarial drug is synthesized in the specialized 10-celled biseriate glandular trichomes of some Artemisia species. In order to have an insight into artemisinin biosynthesis in species other than A. annua, five species with different artemisinin contents were investigated for the expression of key genes that influence artemisinin content. The least relative expression of the examined terpene synthase genes accompanied with very low glandular trichome density (4 No. mm−2) and absence of artemisinin content in A. khorassanica (S2) underscored the vast metabolic capacity of glandular trichomes. A. deserti (S4) with artemisinin content of 5.13 mg g−1 DW had a very high expression of Aa-ALDH1 and Aa-CYP71AV1 and low expression of Aa-DBR2. It is possible to develop plants with high artemisinin synthesis ability by downregulating Aa-ORA in S4, which may result in the reduction of Aa-ALDH1 and Aa-CYP71AV1 genes expression and effectively change the metabolic flux to favor more of artemisinin production than artemisinic acid. Based on the results, the Aa-ABCG6 transporter may be involved in trichome development. S4 had high transcript levels and larger glandular trichomes (3.46 fold) than A. annua found in Iran (S1), which may be due to the presence of more 2C-DNA (3.48 fold) in S4 than S1.

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“…absinthium had a higher expression level of both Aa - ALDH1 and Aa - CYP71AV1 genes when compared to A . annua during the developmental stages 5 . Salehi et al .…”

Section: Introductionmentioning

confidence: 99%

Expression of key genes affecting artemisinin content in five Artemisia species

Salehi

Karimzadeh

Naghavi

et al. 2018

Sci Rep

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“…Regarding the importance of the ADS and DBR2 genes in the production of artemisinin, it might be concluded that the main reason for not producing artemisinin in A. khorassanica is related to the extremely low expression level of the key genes of ADS and DBR2 compared to that in A. annua. Similarly, all previous studies reported that other Artemisia species produced less artemisinin content than A. annua L. (Arab et al, 2006;Hsu, 2006;Zia et al, 2007;Mannan et al, 2010;Ranjbar et al, 2015;Salehi et al, 2018). …”

Section: Discussionmentioning

confidence: 54%

“…Moreover, the expression of Aa-DBR2 was reported in A. absinthium (Muangphrom et al, 2014). The artemisinin biosynthetic pathway (Aa-ADS, Aa-CYP71AV1, Aa-ALDH1, Aa-DBR2, and Aa-RED1) was studied in eight Artemisia species, where A. absinthium showed a higher expression level of both genes Aa-ALDH1 and Aa-CYP71AV1 compared to A. annua at all developmental stages (Ranjbar et al, 2015). Five Artemisia species with different artemisinin contents were studied and it was reported that the artemisinin content of A. deserti (S4, 5.30 mg g -1 DW) was significantly the same as that of A. annua of Iran (S1, 6.27 mg g -1 DW) and less than that of A. annua cv ' Anamed' (14.50 mg g -1 DW) despite having the highest glandular trichome area per leaf and high relative expression of Aa-ADS, Aa-ALDH1, Aa-CYP71AV1, Aa-TTG1, and Aa-TFAR1 (Salehi et al, 2018).…”

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confidence: 98%

Assessment of Iranian endemic Artemisia khorassanica : karyological, genome size, and gene expressions involved in artemisinin production

Hamidi¹,

Karimzadeh²,

Monfared³

et al. 2018

Turk J Biol

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IntroductionArtemisia L. is the largest genus of small herbs and shrubs belonging to the family Asteraceae. It comprises over 500 species, which are mainly found in Asia, Europe, and North America (Bora and Sharma, 2011). Asia has the greatest concentration of species and there are 35 species of this genus in Iran (Abad et al., 2012), some of which are endemic to Iran, e.g., A. khorassanica. Data about karyotypes can be helpful in species recognition and analysis of hybrid populations in genetic and breeding studies (Anjali and Srivastava, 2012). The genus Artemisia has two basic chromosome numbers of x = 9, present in all the subgenera, and the less frequent x = 8, present in some subgenera (Oliva and Vallès, 1994;McArthur and Sanderson, 1999;Dolatyari et al., 2013). The reason for this descending dysploidy, reducing the basic chromosome number from x = 9 to 8, may have been a centric Robertsonian chromosome fusion, as is widely accepted (Vallès and Siljak-Yakovlev, 1997;Pellicer et al., 2011). Moreover, a basic number of x = 7 was previously reported for Artemisia pattersonii by Wiens and Richter (1966). Flow cytometry is known as the most reliable and quick method for estimating the genome size (2C DNA) for a wide range of plant communities (

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“…In Humulus lupulus L, the expression of HlSTS1 encoding for the sesquiterpene synthases which catalyse the formation of caryophyllene and humulene was abundant in those tissues with high contents of humulene and caryophyllene [33]. The higher amounts of artemisinin, which is produced by Artemisia annua , was mainly a result of higher expression of amorpha-4,11-diene synthase (ADS) and artemisinic aldehyde, was a result of higher expression of the amorpha-4,11-diene synthase (ADS) and artemisinic aldehyde Δ11-13 reductase (DBR2) genes [34].…”

Section: Discussionmentioning

confidence: 99%

Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba

et al. 2016

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Abstract:Mountain pepper (Litsea cubeba (Lour.) Pers.) (Lauraceae) is an important industrial crop as an ingredient in cosmetics, pesticides, food additives and potential biofuels. These properties are attributed to monoterpenes and sesquiterpenes. However, there is still no integrated model describing differentially expressed genes (DEGs) involved in terpenoid biosynthesis during the fruit development of L. cubeba. Here, we performed digital gene expression (DGE) using the Illumina NGS platform to evaluated changes in gene expression during fruit development in L. cubeba. DGE generated expression data for approximately 19354 genes. Fruit at 60 days after flowering (DAF) served as the control, and a total of 415, 1255, 449 and 811 up-regulated genes and 505, 1351, 1823 and 1850 down-regulated genes were identified at 75, 90, 105 and 135 DAF, respectively. Pathway analysis revealed 26 genes involved in terpenoid biosynthesis pathways. Three DEGs had continued increasing or declining trends during the fruit development. The quantitative real-time PCR (qRT-PCR) results of five differentially expressed genes were consistent with those obtained from Illumina sequencing. These results provide a comprehensive molecular biology background for research on fruit development, and information that should aid in metabolic engineering to increase the yields of L. cubeba essential oil.

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Expression of artemisinin biosynthesis genes in eight Artemisia species at three developmental stages

Cited by 29 publications

References 56 publications

Expression of key genes affecting artemisinin content in five Artemisia species

Expression of key genes affecting artemisinin content in five Artemisia species

Assessment of Iranian endemic Artemisia khorassanica : karyological, genome size, and gene expressions involved in artemisinin production

Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba

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