Expression of aromatase and oestrogen receptors in reproductive tissues of the stallion and a single cryptorchid visualised by means of immunohistochemistry

Hejmej, Anna; Gorazd, Monika; Kosiniak-Kamysz, K.; Wiszniewska, Barbara; Sadowska, J; Bilińska, Barbara

doi:10.1016/j.domaniend.2005.03.002

Cited by 53 publications

(45 citation statements)

References 48 publications

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“…These findings reinforce that estrogens are involved in epididymal function (Hess et al, 1997) and corroborate the findings of Parlevliet et al (2006) with respect to ER␤. Hejmej et al (2005) also evaluated the intensity of the staining; although our findings were similar for ER␤, the intensity of the staining was stronger in the present than previous study. Findings in the present study differ from those of Parlevliet et al (2006) and Hejmej et al (2005) with respect to ER␣, because no immunostaining was found in the corpus and cauda of epididymides.…”

Section: Discussioncontrasting

confidence: 54%

Immunohistochemical localization of estrogen α and β receptors in adult stallion epididymis

Martin¹,

Melo²,

Rodrigues³

et al. 2010

Animal Reproduction Science

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Section: Discussioncontrasting

confidence: 54%

Immunohistochemical localization of estrogen α and β receptors in adult stallion epididymis

Martin¹,

Melo²,

Rodrigues³

et al. 2010

Animal Reproduction Science

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“…However, different research results are not in accordance when concerning the localization of ERα and ERβ, particularly in the testis [3]. It was shown in many studies that after birth, in the neonatal and peripubertal period of life, the expression of ERα is limited to the Leydig cells in rodents and stallions [4] but the expression of ERβ in rats is present in germ and somatic testicular cells, including Leydig or Sertoli cells [5,6]. In 4-day-old rats fetal germ cells (gonocytes) reveal ERβ mRNA in their cytoplasm and ERβ protein in their nucleus.…”

Section: Introductionmentioning

confidence: 94%

Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.

Filipiak

Walczak-Jędrzejowska²,

Oszukowska³

et al. 2010

Folia Histochem Cytobiol.

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Abstract:The aim of this study was to assess the impact of xenoestrogens: diethylstilbestrol (DES) and zearalenone (ZEA) on rat's pubertal testis and to compare it with the effect of natural estrogen -17β-estradiol (E). Male Wistar rats were daily, subcutaneously injected at 5 th -15 th postnatal days (p.d.) with E (1.25 or 12.5 μg) or DES (1.25 or 12.5 μg) or ZEA (4 or 40 μg) or vehicle. At 16 th p.d. testes were dissected, weighted, and paraffin embedded. Following parameters were assessed: diameter and length of seminiferous tubule, numbers of spermatogonia A+intermediate+B (A/In/B), preleptotene spermatocytes (PL), leptotene+zygotene+pachytene spermatocytes (L/Z/PA) and Sertoli cells per testis. Testes weight, seminiferous tubule diameter and length were decreased by both doses of E, DES and ZEA. DES effect was the strongest, but its influence on testis weight and seminiferous tubule length, on the contrary to E and ZEA, was not dose-dependent. Similarly, DES in both doses had the most severe negative impact on the number of germ and Sertoli cells. The negative influence of E on germ cells was less pronounced. The negative effect of ZEA was seen only after administration of the higher dose on spermatogonia number, while DES and E decreased A/In/B number more evidently. Sertoli cell number were decreased after both doses of E. ZEA40 decreased Sertoli cell number while ZEA4 had no effect. Conclusion: exposure of prepubertal male rat to DES has the strongest detrimental effect on the developing testis in comparison to E and ZEA. Both, E and DES, decreased number of germ and Sertoli cells, diminished seminiferous tubule diameter, length and testis weight. ZEA had much more weaker effect than the potent estrogens.

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“…ERa and ERb localization changes during the lifespan of rodents and stallions [14,15]. According to Hejmej et al [16] the presence of ERa in stallions is limited to Leydig cells during the neonatal and peripubertal periods of life. In the bank vole (independently of the animal's age) ERa presence was observed in Leydig cells while ERb was present in Sertoli cells, and also in the nuclei of spermatocytes and elongated spermatids of animals exposed to long light cycles [17].…”

Section: Introductionmentioning

confidence: 99%

Estrogen receptor alpha localization in the testes of men with normal spermatogenesis

Filipiak¹,

Suliborska²,

Walczak-Jędrzejowska³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:It is known that estrogens act on the male reproductive tract by binding to estrogen receptors (ER) a and b. However, studies on ER localization in the human testis are discordant. The aim of this study was to investigate the localization of ERa in the testes of adult men with normal spermatogenesis. Semen analysis of ten adult men revealed azoospermia. FSH, LH and testosterone serum concentrations were within normal values, and the volume of the testes was normal, hence obstructive azoospermia was suspected. The tissues from testicular surgical biopsies were fixed in Bouin's fluid and embedded in paraffin. Assessments of the seminiferous epithelium (scoring 10 to -1), the number of Leydig cells (scoring 1 to 5), the areal fraction of intertubular space (IS), measurements of seminiferous tubule diameter, and the thickness of the tubular wall, were performed on microscopic sections. Immunohistochemical staining was applied with monoclonal antibodies against ERa. The mean spermatogenesis score was 10 points; IS -30.6 ± 8.1%; seminiferous tubule diameter -193.9 ± 19.4 μm; thickness of tubular wall -7.44 ± 1.1 μm; number of Leydig cells -1.6 ± 1.1 points. Immunohistochemical staining showed the localization of ERa to be in the Sertoli and Leydig cell cytoplasm, while ERa was absent in germ cells. The results of testicular tissue analysis confirmed its normal structure and normal, full spermatogenesis. The presence of ERa in Sertoli and Leydig cells in normal human testis demonstrated in this study suggests that estrogens may affect testicular function.

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Expression of aromatase and oestrogen receptors in reproductive tissues of the stallion and a single cryptorchid visualised by means of immunohistochemistry

Cited by 53 publications

References 48 publications

Immunohistochemical localization of estrogen α and β receptors in adult stallion epididymis

Immunohistochemical localization of estrogen α and β receptors in adult stallion epididymis

Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.

Estrogen receptor alpha localization in the testes of men with normal spermatogenesis

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