Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development

Xiong, Yun; Tan, Ya‐Jing; Xiong, Yi-Meng; Huang, Yi‐Ting; Hu, Xiaoling; Lu, Yongchao; Yao, Yuqi; Wang, Tingting; Zhang, Dan; Jin, Fan; Huang, He‐Feng; Sheng, Jian‐Zhong

doi:10.1159/000350084

Cited by 41 publications

(37 citation statements)

References 27 publications

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“…It has been shown that increased expression of AQP5 occurs at the time of the embryo implantation in the rat uterus (Lindsay and Murphy 2007). Further, multiple aquaporins are expressed in the early stage human embryos and AQP3 and AQP7 may play a role in pre-implantation mouse embryo development (Xiong et al 2013). Implantation of the developing embryo in the uterus is regulated by multiple effectors, such as steroid hormones, growth factors, cytokines and cyclooxygenasederived prostaglandins , Geisert et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Expression of Aquaporin 1 and 5 and Their Regulation by Ovarian Hormones, Arachidonic Acid, Forskolin and cAMP During Implantation in Pigs

Skowrońska

Młotkowska²,

Majewski³

et al. 2016

Physiol Res

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Aquaporin proteins (AQPs) are a family of channels expressed in numerous mammalian tissues, where they play a fundamental role in regulating water transport across cell membranes. Based on reports that AQPs are present in the reproductive system and participate in reproductive processes, our aim was to investigate the effect of progesterone (P4), estradiol (E2), oxytocin (OT), arachidonic acid (AA), forskolin (FSK) and cyclic adenosine monophosphate (cAMP) on AQP1 and AQP5 expression at mRNA and protein levels in porcine uterine explants from Days 14-16 of gestation in order to determine if they play a role in implantation period in pigs. Quantitative real time PCR and Western-blot analysis revealed that the uterine explants treated with FSK and cAMP produce delayed, but long-term effects on AQP1 abundance (24 h) while AQP5 had a rapid and sustained response to FSK and cAMP in protein content (3 and 24 h). AA increases gene and protein content of AQP1 after longer exposition whereas AQP5 increases after 3 h only at the protein level. Both AQPs potentially remains under control of steroid hormones. OT has been shown to increase AQP1, and decrease AQP5 mRNA, without visible changes in protein content. P4, E2, AA, FSK and cAMP caused the appearance of AQP5 expression in the basolateral plasma membrane of the epithelial cells. The staining represents most likely AQP5 functioning mechanism for both absorption and reabsorption across the glandular epithelium.

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Section: Introductionmentioning

confidence: 99%

Expression of Aquaporin 1 and 5 and Their Regulation by Ovarian Hormones, Arachidonic Acid, Forskolin and cAMP During Implantation in Pigs

Skowrońska

Młotkowska²,

Majewski³

et al. 2016

Physiol Res

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“…Lastly, AQP3 or ATP1B1 play an important role in blastocoel formation by regulating the accumulation of fluid. In mouse embryos, RNAi mediated knockdown of Aqp3 and Atp1b1 blocks blastocyst formation [37,38]. Altogether, these results demonstrate that maternal YAP in pig embryos promotes TE development via the regulation of key genes involved in TJ assembly and blastocoel formation.…”

Section: Discussionmentioning

confidence: 60%

Maternal Yes-Associated Protein Participates in Porcine Blastocyst Development via Modulation of Trophectoderm Epithelium Barrier Function

Cao

et al. 2019

Cells

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The establishment of a functional trophectoderm (TE) epithelium is an essential prerequisite for blastocyst formation and placentation. Transcription coactivator yes-associated protein (YAP), a downstream effector of the hippo signaling pathway, is required for specification of both the TE and epiblast lineages in mice. However, the biological role of YAP in porcine blastocyst development is not known. Here, we report that maternally derived YAP protein is localized to both the cytoplasm and nuclei prior to the morula stage and is then predominantly localized to the TE nuclei in blastocysts. Functionally, maternal YAP knockdown severely impeded blastocyst formation and perturbed the allocation of the first two lineages. The treatment of embryos with verteporfin, a pharmacological inhibitor of YAP, faithfully recapitulated the phenotype observed in YAP deleted embryos. Mechanistically, we found that maternal YAP regulates multiple genes which are important for lineage commitment, tight junction assembly, and fluid accumulation. Consistent with the effects on tight junction gene expression, a permeability assay revealed that paracellular sealing was defective in the trophectoderm epithelium. Lastly, YAP knockdown in a single blastomere at the 2-cell stage revealed that the cellular progeny of the YAP + blastomere were sufficient to sustain blastocyst formation via direct complementation of the defective trophectoderm epithelium. In summary, these findings demonstrate that maternal YAP facilitates porcine blastocyst development through transcriptional regulation of key genes that are essential for lineage commitment, tight junction assembly, and fluid accumulation.

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“…The role of aquaporins (AQPs), water channel proteins involved in regulation of cellular water homeostasis, was studied in sea urchin, mouse, and human embryos, demonstrating their expression and implication in mammalian preimplantation embryo development (Edashige et al, 2000;Amaroli et al, 2013;Nong et al, 2013;Xiong et al, 2013). Members of the AQPs family take part in the processes of blastocyst formation as physiological mediators of fluid movement across the trophectoderm during cavitation and implantation (Cho et al, 2003;Richard et al, 2003;Watson et al, 2004).…”

Section: Mammalsmentioning

confidence: 99%

Ion currents in embryo development

Tosti

Boni

Gallo

2016

Birth Defects Research Pt C

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Ion channels are proteins expressed in the plasma membrane of electrogenic cells. In the zygote and blastomeres of the developing embryo, electrical modifications result from ion currents that flow through these channels. This phenomenon implies that ion current activity exerts a specific developmental function, and plays a crucial role in signal transduction and the control of embryogenesis, from the early cleavage stages and during growth and development of the embryo. This review describes the involvement of ion currents in early embryo development, from marine invertebrates to human, focusing on the occurrence, modulation, and dynamic role of ion fluxes taking place on the zygote and blastomere plasma membrane, and at the intercellular communication between embryo cell stages.Birth Defects Research (Part C) 108:6-18, 2016.V C 2016 Wiley Periodicals, Inc.

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Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development

Cited by 41 publications

References 27 publications

Expression of Aquaporin 1 and 5 and Their Regulation by Ovarian Hormones, Arachidonic Acid, Forskolin and cAMP During Implantation in Pigs

Expression of Aquaporin 1 and 5 and Their Regulation by Ovarian Hormones, Arachidonic Acid, Forskolin and cAMP During Implantation in Pigs

Maternal Yes-Associated Protein Participates in Porcine Blastocyst Development via Modulation of Trophectoderm Epithelium Barrier Function

Ion currents in embryo development

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