Expression of aquaporin 5 increases proliferation and metastasis potential of lung cancer

Zhang, Ziqiang; Chen, Zhihong; Song, Yuanlin; Zhang, Pinghai; Hu, Jie; Bai, Chunxue

doi:10.1002/path.2702

Cited by 132 publications

(132 citation statements)

References 53 publications

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“…As possible mechanisms, the strength of the cytoskeleton or the membrane, or the expression of the Cl -channels may differ among these ESCC cell lines. Further, overexpression of several types of aquaporins (AQP), transmembrane water channel proteins which play an important role in transcellular water movement, has been reported in different types of human cancer (24)(25)(26)(27). cAMP has been reported to up-regulate AQP5 expression in lung epithelial cells, hence increasing water permeability (28).…”

Section: Discussionmentioning

confidence: 99%

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga

Shiozaki

Ichikawa³

et al. 2011

Oncol Rep

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Abstract. This study aimed to investigate cytocidal effects of hypotonic shock on esophageal squamous cell carcinoma (ESCC) cell lines, and to apply pleural lavage with distilled water to surgery for ESCC. Three human ESCC cell lines, TE5, TE9 and KYSE170 were exposed to distilled water, and morphological changes in ESCC cells were closely observed under a differential interference contrast microscope connected to a high-speed digital video camera. Further, serial cell volume changes after hypotonic shock were measured using a high-resolution flow cytometer. To investigate the cytocidal effects of hypotonic shock on ESCC cells, re-incubation of ESCC cells was performed after hypotonic shock. Additionally, the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), a Cl -channel blocker, during hypotonic shock were analyzed. Video recordings by high-speed digital camera demonstrated that hypotonic shock with distilled water induced cell swelling followed by cell rupture. Measurements of cell volume changes using a high-resolution flow cytometer indicated that severe hypotonicity with distilled water increased broken fragments of ESCC cells within 5 min. Re-incubation experiments demonstrated cytocidal effects of hypotonic shock on ESCC cells. Treatment of cells with NPPB increased cell volumes by the inhibition of regulatory volume decrease, which is observed during hypotonic shock, and enhanced cytocidal effects. These findings demonstrated the cytocidal effects of hypotonic shock on ESCC cells, and clearly support the efficacy of pleural lavage with distilled water during surgery for ESCC.

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Section: Discussionmentioning

confidence: 99%

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga

Shiozaki

Ichikawa³

et al. 2011

Oncol Rep

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show abstract

“…Notably, the present study suggested that AQPs, including AQP5, are involved in tumorigenesis. AQP5, a 21-24 kDa protein, was initially described as the main structural protein in caveolae and was believed to be a key molecule involved in oncogenic transformation and malignant progression (14,15). AQP5 plays an important regulatory role in several signaling pathways leading to cellular transformation, including those mediated by the Src family of tyrosine kinases, epidermal growth factor receptor, Wnt, and Erk1/2 (10,16,17).…”

Section: Discussionmentioning

confidence: 99%

AQP5: A novel biomarker that predicts poor clinical outcome in colorectal cancer

Shan

Cui

et al. 2014

Oncology Reports

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Abstract. The aquaporins (AQPs) are water channel proteins that exhibit several properties related to tumor development. However, the expression and clinical significance of AQP5 in colorectal cancer, particularly the correlation with circulating tumor cells (CTCs), has not been elucidated. The aim of the present study was to determine whether or not the expression of AQP5 is a strong prognostic biomarker for colorectal cancer. The results showed that of 45 tumor specimens, 14 (31.1%) had high levels of expression of AQP5, 29 (64.4%) exhibited a moderate (intermediate) level of staining, and 2 (4.4%) had an absence of AQP5 staining. AQP5 was only occasionally detected in para-neoplastic [3/45 (6.67%)] and normal tissues [3/45 (6.67%)]. AQP5 protein overexpression frequently accompanied gene amplification detection with fluorescence in situ hybridization (FISH). Moreover, AQP5 expression in colorectal cancer cells was upregulated compared to normal colon cells. AQP5 overexpression was also associated with TNM stage (P=0.002), lymph node metastasis (P=0.016), and distant metastasis (P=0.000). The relationships between age, gender, histologic grade and tumor size with expression of AQP5 were not significant (P>0.05). A positive correlation between the number of CTCs and AQP5 expression (P<0.05) was demonstrated. In addition, patients who did not express AQP5 had a superior cumulative survival rate compared to patients with AQP5 positivity. AQP5 may be used as a novel biomarker for colorectal cancer aggressiveness and metastasis, but it does not reflect drug resistance.

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“…We previously reported that AQP-5 may be involved in differentiation of human gastric carcinoma cells, and overexpression of several aquaporins has been reported in different types of human carcinoma (8)(9)(10)(11)(12)(13)(14)(15)(16). However, the role of AQPs in biliary tract carcinogenesis has not yet been clearly defined.…”

Section: Introductionmentioning

confidence: 99%

Prognostic significance of aquaporins in human biliary tract carcinoma

et al. 2012

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Abstract. Aquaporins (AQPs) are important in controlling bile formation, however, the exact role of AQPs in human biliary tract carcinogenesis has not been clearly defined. In this study, we analyzed AQP-1, -4, -5 and -8 expression immunohistochemically using tissue microarrays (TMAs) in 81 samples. (45 gallbladder carcinomas and 36 bile duct carcinomas). The survival of patients with high AQP-5 expression was longer compared to that of patients with low AQP-5 expression (P=0.017). Cox's proportional hazard model revealed that AQP-5 expression was an independent prognostic factor (RR, 0.38; P=0.025). Chi-square analysis revealed that high AQP-5 expression correlated to small tumor size in biliary tract carcinoma patients (P=0.006). With regard to the expression of other AQPs, depth of tumor invasion, histological type and serum carbohydrate antigen 19-9 (CA19-9) were associated with high AQP-1 expression (P=0.039, 0.011 and 0.032). However, AQP-4 and AQP-8 expression had no association with clinicopathological factors. Among the 10 patients who underwent gemcitabine (GEM) plus S-1 postoperative chemotherapy, the group of patients (n=5) with high AQP-5 expression were associated with higher rates of both overall and disease-free survival (log-rank P=0.033, 0.002). In conclusion, the results of this study suggest that AQP-5 expression may be associated with prognosis and drug sensitivity in biliary tract carcinoma.

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Expression of aquaporin 5 increases proliferation and metastasis potential of lung cancer

Cited by 132 publications

References 53 publications

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

AQP5: A novel biomarker that predicts poor clinical outcome in colorectal cancer

Prognostic significance of aquaporins in human biliary tract carcinoma

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