Expression of AoNHX1 increases salt tolerance of rice and Arabidopsis, and bHLH transcription factors regulate AtNHX1 and AtNHX6 in Arabidopsis

Krishnamurthy, Pannaga; Vishal, Bhushan; Khoo, Kaijie; Rajappa, Sivamathini; Loh, Chiang‐Shiong; Kumar, Prakash P.

doi:10.1007/s00299-019-02450-w

Cited by 52 publications

(27 citation statements)

References 67 publications

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“…Regulates the salt tolerance gene AtNHX6 and AtNHX6 in roots. Promotes ABA and JA responsiveness [45][46][47][48][49].…”

Section: Resultsmentioning

confidence: 99%

“…For example, members in subfamilies S1, S4, and S12 affect iron uptake [44,52,57]. Studies have also found that AtbHLH17/AIB (S2), AtbHLH92 (S7), AtbHLH112 (S15), and bHLH122 (S27) respond to salt stress [45,56,60,76], while AtbHLH68 (S15) and ZmPTF1 (S26) respond to drought stress [61,69]. Meanwhile, many MYC2 homologous genes in the S2 subfamily (e.g., Catharanthus roseus CrMYC2 [46] and Salvia miltiorrhiza SmMYC2 [47]) are known to be involved in cellular responses to ABA and JA [20][21][22].…”

Section: Phylogenetic Tree and Subfamily Divisionmentioning

confidence: 99%

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Genome-wide survey of the bHLH super gene family in Brassica napus

Zhou

et al. 2020

BMC Plant Biol

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Background: The basic helix-loop-helix (bHLH) gene family is one of the largest transcription factor families in plants and is functionally characterized in diverse species. However, less is known about its functions in the economically important allopolyploid oil crop, Brassica napus. Results: We identified 602 potential bHLHs in the B. napus genome (BnabHLHs) and categorized them into 35 subfamilies, including seven newly separated subfamilies, based on phylogeny, protein structure, and exon-intron organization analysis. The intron insertion patterns of this gene family were analyzed and a total of eight types were identified in the bHLH regions of BnabHLHs. Chromosome distribution and synteny analyses revealed that hybridization between Brassica rapa and Brassica oleracea was the main expansion mechanism for BnabHLHs. Expression analyses showed that BnabHLHs were widely in different plant tissues and formed seven main patterns, suggesting they may participate in various aspects of B. napus development. Furthermore, when roots were treated with five different hormones (IAA, auxin; GA 3 , gibberellin; 6-BA, cytokinin; ABA, abscisic acid and ACC, ethylene), the expression profiles of BnabHLHs changed significantly, with many showing increased expression. The induction of five candidate BnabHLHs was confirmed following the five hormone treatments via qRT-PCR. Up to 246 BnabHLHs from nine subfamilies were predicted to have potential roles relating to root development through the joint analysis of their expression profiles and homolog function. Conclusion: The 602 BnabHLHs identified from B. napus were classified into 35 subfamilies, and those members from the same subfamily generally had similar sequence motifs. Overall, we found that BnabHLHs may be widely involved in root development in B. napus. Moreover, this study provides important insights into the potential functions of the BnabHLHs super gene family and thus will be useful in future gene function research.

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“…Regulates the salt tolerance gene AtNHX6 and AtNHX6 in roots. Promotes ABA and JA responsiveness [45][46][47][48][49].…”

Section: Resultsmentioning

confidence: 99%

Section: Phylogenetic Tree and Subfamily Divisionmentioning

confidence: 99%

Genome-wide survey of the bHLH super gene family in Brassica napus

Zhou

et al. 2020

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…For example, members in subfamilies S1, S4, and S12 affect iron uptake [58][59][60]. Studies have also found that AtbHLH17/AIB (S2), AtbHLH92 (S7), AtbHLH112 (S15), and bHLH122 (S27) respond to salt stress [55,[61][62][63], while AtbHLH68 (S15) and ZmPTF1 (S26) respond to drought stress [47,64]. Meanwhile, many MYC2 homologous genes in the S2 subfamily (e.g., Catharanthus roseus CrMYC2 [65] and Salvia miltiorrhiza SmMYC2 [66]) are known to be involved in cellular responses to ABA and JA [20][21][22].…”

Section: The Potential Roles Of Bhlhs In B Napus Rootsmentioning

confidence: 99%

Genome-wide Survey of the bHLH Super Gene Family in Brassica napus

Zhou

et al. 2020

Preprint

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Background: The basic helix-loop-helix (bHLH) gene family is one of the largest transcription factor families in plants and is functionally characterized in diverse species. However, less is known about its functions in the economically important allopolyploid oil crop, Brassica napus. Results: We identified 602 potential bHLHs in the B. napus genome (BnabHLHs) and categorized them into 35 subfamilies, including seven newly separated subfamilies, based on phylogeny, protein structure, and exon-intron organization analysis. The intron insertion patterns of this gene family were analyzed and a total of eight types were identified in the bHLH regions of BnabHLHs. Chromosome distribution and synteny analyses revealed that hybridization between Brassica rapa and Brassica oleracea was the main expansion mechanism for BnabHLHs. Expression analyses showed that BnabHLHs were widely in different plant tissues and formed seven main patterns, suggesting they may participate in various aspects of B. napus development. Furthermore, when roots were treated with five different hormones (IAA, auxin; GA3, gibberellin; 6-BA, cytokinin; ABA, abscisic acid and ACC, ethylene), the expression profiles of BnabHLHs changed significantly, with many showing increased expression. The induction of five candidate BnabHLHs was confirmed following the five hormone treatments via qRT-PCR. Up to 246 BnabHLHs from nine subfamilies were predicted to have potential roles relating to root development through the joint analysis of their expression profiles and homolog function. Conclusion: The 602 BnabHLHs identified from B. napus were classified into 35 subfamilies, and those members from the same subfamily generally had similar sequence motifs. Overall, we found that BnabHLHs may be widely involved in root development in B. napus. Moreover, this study provides important insights into the potential functions of the BnabHLHs super gene family and thus will be useful in future gene function research.

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