Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV

Piñeyro, Pablo; Kenney, Scott P.; Giménez-Lirola, Luis; Heffron, C. Lynn; Matzinger, Shannon R.; Opriessnig, Tanja; Meng, Xiang‐Jin

doi:10.1016/j.virusres.2015.07.027

Cited by 16 publications

(21 citation statements)

References 63 publications

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“…Recently, significant progress has been made regarding the development of new vaccination strategies against PRRSV2021. Recombinant viral genome vaccines against PRRSV may represent an alternative for the generation of live, attenuated viral vaccines22. The swinepox virus (SPV) is a promising vaccine vector due to its ability to stimulate the immune response, and its capacity for heterogeneous insertions2324.…”

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confidence: 99%

Construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus

Lin

Hou

et al. 2017

Sci Rep

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To characterize neutralizing mimotopes, phages were selected from a 12-mer phage display library using three anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibodies: (1) A1; (2) A2; and (3) A7. Of these, A2 and A7 recognize the mimotope, P2, which contains the SRHDHIH motif, which has conserved consensus sequences from amino acid positions 156 to 161 in the N-terminal ectodomain of GP3. The artificial multi-epitope gene, mp2, was designed by combining three repeats of the mimotope P2. The resulting sequence was inserted into the swinepox virus (SPV) genome to construct a recombinant swinepox virus (rSPV-mp2). The rSPV-mp2 was able to stably express the multi-epitope peptide, mP2, in vitro. The rSPV-mp2 immunized pigs exhibited a significantly shorter fever duration compared with the wtSPV treated group (P < 0.05). There was an enhanced humoral and cellular immune response, decreased number of PRRSV genomic copies, and a significant reduction in the gross lung pathology (P < 0.05) was observed following PRRSV infection in rSPV-mp2-immunized animals. The results suggest that the recombinant rSPV-mp2 provided pigs with significant protection against PRRSV infection.

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confidence: 99%

Construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus

Lin

Hou

et al. 2017

Sci Rep

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“…Owing to the poor immunogenicity of CTL epitopes derived from tumor antigens, altering the amino-acid residues that interact with either HLA molecules or the T-cell receptor is one way to enhance immunogenicity. [24][25][26] Such a strategy has been demonstrated to significantly increase protective and therapeutic immunity against tumors in clinical trials. 31,32 Here, we engineered epitope-enhanced analogs of the native P40-48 peptide by replacing amino acids at positions 1, 3, 6 and 7, which are known to contain key residues.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have indicated that residues at positions 1, 2, 3, 6, 7 and 9 may be replaced. [24][25][26] Residues at positions 2 and 9 are primary anchor motifs, and those at positions 1, 3, 6 and 7 are secondary anchor motifs. The native P40-48 peptide already contains preferred residues (leucine (L), valine (V)) at positions 2 and 9; we therefore used in silico analysis to determine further potential improvement in the ability of the peptide to bind to HLA-A0201 molecules.…”

Section: Screening Of Potential Hpebp4-derived Peptides That Bind To mentioning

confidence: 99%

A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses

Sun

Shi

et al. 2018

Cell Mol Immunol

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We previously identified human phosphatidylethanolamine-binding protein 4 (hPEBP4) as an antiapoptotic protein with increased expression levels in breast, ovarian and prostate cancer cells, but low expression levels in normal tissues, which makes hPEBP4 an attractive target for immunotherapy. Here, we developed hPEBP4-derived immunogenic peptides for inducing antigen-specific cytotoxic T lymphocytes (CTLs) targeting breast cancer. A panel of hPEBP4-derived peptides predicted by peptide-MHC-binding algorithms was evaluated to characterize their HLA-A2.1 affinity and immunogenicity. We identified a novel immunogenic peptide, P40-48 (TLFCQGLEV), that was capable of eliciting specific CTL responses in HLA-A2.1/K transgenic mice, as well as in peripheral blood lymphocytes from breast cancer patients. Furthermore, amino-acid substitutions in the P40-48 sequence improved its immunogenicity against hPEBP4, a self-antigen, thus circumventing tolerance. We designed peptide analogs by preferred auxiliary HLA-A*0201 anchor residue replacement, which induced CTLs that were crossreactive to the native peptide. Several analogs were able to stably bind to HLA-A*0201 and elicit specific CTL responses better than the native sequence. Importantly, adoptive transfer of CTLs induced by vaccination with two analogs more effectively inhibited tumor growth than the native peptide. These data indicate that peptide analogs with high immunogenicity represent promising candidates for peptide-mediated therapeutic cancer vaccines.

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“…However, recently both diseases have caused great economic losses to the porcine industry (Piñeyro et al, 2015). PCV2 mainly affects animals between 5 and 15 weeks of age, inducing among several signs growth retardation and respiratory diseases (Segalés et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Study of porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) frequencies and coinfection in mexican farrow to finish pig farms

Vargas¹,

Escatell²,

Lara³

et al. 2018

J. Vet. Med. Anim. Health

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The porcine circovirus type 2 (PCV2) is commonly present along with the porcine reproductive and respiratory syndrome virus (PRRSV). These are the most important pathogens for pig industry worldwide. In this study, we determined frequencies for both viruses, as well as coinfection, in farrow to finish farms from México. For this, pigs from 28 farms in different states were sampled for tonsils, lungs and lymphatic nodes and assayed for PCV2 and PRRSV by polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR). Herds detected positive were: 16 PCV2 (57%), 10 PRRSV (36%); and 5 had both viruses (18%). Samples detected positives were: 49% PCV2, and 39% PRRSV. Coinfection frequency observed in this work contrasted those observed in other countries. We think that these data will contribute to a better understanding of both diseases in order to take better measures for preventing and controlling them.

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Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV

Cited by 16 publications

References 63 publications

Construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus

Construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus

A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses

Study of porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) frequencies and coinfection in mexican farrow to finish pig farms

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