Expression of angiotensin II type 1 (AT1) and angiotensin II type 2 (AT2) receptors in human granulosa-lutein (GL) cells: correlation with infertility diagnoses

Peña, O.; Palumbo, Angela; González-Fernández, Rebeca; Hernández, Jairo; Naftolin, Frederick; Ávila, Julio

doi:10.1016/j.fertnstert.2009.03.092

Cited by 18 publications

(19 citation statements)

References 34 publications

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“…While convincing of differences in mural hGL cells, these findings do not reveal sirtuin family expression in the cumulus cells that are directly in apposition to the oocyte/embryo. We have shown that there are important biochemical differences between mural and cumulus cells [64,65]. Additionally, there have been reports of miRNA differences in poor responders [66] and other genes' expression in cumulus cells from obese women [67,68]; the possibility of mural vs. cumulus differences is on the list for further study.…”

Section: These Findings Have Implications For Ovarian Agingmentioning

confidence: 81%

Granulosa-Lutein Cell Sirtuin Gene Expression Profiles Differ between Normal Donors and Infertile Women

González-Fernández

Martín-Ramírez

Rotoli

et al. 2019

IJMS

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Sirtuins are a family of deacetylases that modify structural proteins, metabolic enzymes, and histones to change cellular protein localization and function. In mammals, there are seven sirtuins involved in processes like oxidative stress or metabolic homeostasis associated with aging, degeneration or cancer. We studied gene expression of sirtuins by qRT-PCR in human mural granulosa-lutein cells (hGL) from IVF patients in different infertility diagnostic groups and in oocyte donors (OD; control group). Study 1: sirtuins genes’ expression levels and correlations with age and IVF parameters in women with no ovarian factor. We found significantly higher expression levels of SIRT1, SIRT2 and SIRT5 in patients ≥40 years old than in OD and in women between 27 and 39 years old with tubal or male factor, and no ovarian factor (NOF). Only SIRT2, SIRT5 and SIRT7 expression correlated with age. Study 2: sirtuin genes’ expression in women poor responders (PR), endometriosis (EM) and polycystic ovarian syndrome. Compared to NOF controls, we found higher SIRT2 gene expression in all diagnostic groups while SIRT3, SIRT5, SIRT6 and SIRT7 expression were higher only in PR. Related to clinical parameters SIRT1, SIRT6 and SIRT7 correlate positively with FSH and LH doses administered in EM patients. The number of mature oocytes retrieved in PR is positively correlated with the expression levels of SIRT3, SIRT4 and SIRT5. These data suggest that cellular physiopathology in PR’s follicle may be associated with cumulative DNA damage, indicating that further studies are necessary.

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Section: These Findings Have Implications For Ovarian Agingmentioning

confidence: 81%

Granulosa-Lutein Cell Sirtuin Gene Expression Profiles Differ between Normal Donors and Infertile Women

González-Fernández

Martín-Ramírez

Rotoli

et al. 2019

IJMS

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“…These findings indicate that FSH and hCG may mediate ovarian RAS activation [ 48 ]. Moreover, negative correlation between expression of AT1 receptor in FF granulosa cells and the number of mature oocytes was observed in women with poor ovarian reserve [ 63 ].…”

Section: The Relationship Between Gonadotropins Sex Hormones Andmentioning

confidence: 99%

Involvement of ACE2/Ang-(1-7)/MAS1 Axis in the Regulation of Ovarian Function in Mammals

Domińska

2020

IJMS

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In addition to the classic, endocrine renin-angiotensin system, local renin-angiotensin system (RAS) has been documented in many tissues and organs, including the ovaries. The localization and functional activity of the two opposing axes of the system, viz. ACE1/Ang II/AT1 and ACE2/Ang-(1-7)/MAS1, differs between animal species and varied according to the stage of follicle development. It appears that the angiotensin peptides and their receptors participate in reproductive processes such as folliculogenesis, steroidogenesis, oocyte maturation, and ovulation. In addition, changes in the constituent compounds of local RAS may contribute to pathological conditions, such as polycystic ovary syndrome, ovarian hyperstimulation syndrome, and ovarian cancer. This review article examines the expression, localization, metabolism, and activity of individual elements of the ACE2/Ang-(1-7)/MAS1 axis in the ovaries of various animal species. The manuscript also presents the relationship between the secretion of gonadotropins and sex hormones and expression of Ang-(1-7) and MAS1 receptors. It also summarizes current knowledge regarding the positive and negative impact of ACE2/Ang-(1-7)/MAS1 axis on ovarian function.

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“…Real-time quantitative PCR were performed with cDNA using a 7500 Real-Time PCR System (Applied Biosystems). The primer sequence of the AT1 receptor was AGC CAG CGT CAG TTT CAA CC for the forward sequence and ACA AGC ATT GTG CGT CGA AG for the reverse sequence [20] . The primer sequence of the AT2 receptor was AGA ACA GGA TAA CCC GTG ACC for the forward sequence and AGG ATG GCAAAA GGA AGT GC for the reverse sequence [21] .…”

Section: Template Preparation and Qrt-pcrmentioning

confidence: 99%

“…We validate the function of angiotensin II using the AT1/AT2 ratio, according to earlier literature [20] . No significant differences were observed in the eutopic endometria at any phase in either controls or patients with endometriosis.…”

Section: The At1/at2 Ratio In Endometriotic Lesions and Eutopic Endommentioning

confidence: 99%

Expression of Angiotensin II Types 1 and 2 Receptors in Endometriotic Lesions

Nakao

Chishima²,

Sugitani³

et al. 2016

Gynecol Obstet Invest

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Background/Aim: The aim of this study was to evaluate the gene and protein expression of angiotensin type (AT) 1, AT2 receptors in endometriotic lesions and its relation to prostaglandin (PG) synthases. Materials and Methods: Endometriosis samples were obtained from 32 patients with endometriotic cysts. Endometrial tissues were obtained during operations for benign gynecological conditions. The expression of the AT1 and AT2 receptor mRNA and that of PG-endoperoxide synthase 2 and microsomal PGE2 synthase-1 (mPGES-1) was examined by quantitative RT-PCR. Immunohistochemical staining was performed for these receptors. Results: AT1 and AT2 receptor proteins were mostly located in endometrial glandular epithelium and some stromal cells. Immunoreactivity of the receptor proteins was observed in both the eutopic endometrium and endometriotic lesions. The AT1/AT2 ratio in endometriotic cysts (median 7.29, range 1.88-187.60) was significantly increased compared with that in the eutopic endometrium in the proliferative-phase in controls (median 1.01, range 0.37-2.09, p < 0.001). There was a relationship between the AT1 mRNA expression and that of mPGES-1 mRNA in the endometriotic cysts (r = 0.394089, p < 0.05). There was a significant relationship between the mRNA expression of the AT2 receptor and that of mPGES-1 in eutopic endometrium of non-endometriotic control (r = 0.610714, p < 0.05). Conclusion: Renin-angiotensin system may play an important role in the pathophysiology of endometriosis.

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Expression of angiotensin II type 1 (AT1) and angiotensin II type 2 (AT2) receptors in human granulosa-lutein (GL) cells: correlation with infertility diagnoses

Cited by 18 publications

References 34 publications

Granulosa-Lutein Cell Sirtuin Gene Expression Profiles Differ between Normal Donors and Infertile Women

Granulosa-Lutein Cell Sirtuin Gene Expression Profiles Differ between Normal Donors and Infertile Women

Involvement of ACE2/Ang-(1-7)/MAS1 Axis in the Regulation of Ovarian Function in Mammals

Expression of Angiotensin II Types 1 and 2 Receptors in Endometriotic Lesions

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